The Relationship Between The Total Count of Leukocyte Types and Formahldehyde Induction in Wistar Rats

Anak Agung Bagus Tito Indra Prawira Negara; Anak Agung Ayu Niti  Wedayani; Bayu Tirta Dirja

doi:10.29303/jbt.v23i1.6089

The Relationship Between The Total Count of Leukocyte Types and Formahldehyde Induction in Wistar Rats

Authors

Anak Agung Bagus Tito Indra Prawira Negara , Anak Agung Ayu Niti Wedayani , Bayu Tirta Dirja

DOI:

10.29303/jbt.v23i1.6089

Published:

2023-12-04

Issue:

Vol. 23 No. 1 (2023): Special Issue

Keywords:

Formaldehyde, leukocytes, total count of leukocyte types, toxicity, wistar rats.

Articles

Downloads

How to Cite

Negara, A. A. B. T. I. P., Wedayani, A. A. A. N. ., & Dirja, B. T. (2023). The Relationship Between The Total Count of Leukocyte Types and Formahldehyde Induction in Wistar Rats. Jurnal Biologi Tropis, 23(1), 359–363. https://doi.org/10.29303/jbt.v23i1.6089

Download Citation

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Abstract

Formaldehyde is a chemical compound with a high level of toxicity. In the industrial sector, formaldehyde is commonly found in cosmetic products, resins, adhesives, plastics, and wood products. Therefore, the objective of this research is to determine the effect of formaldehyde induction with concentrations of 20 ppm, 30 ppm, and 40 ppm on the total count of leukocyte types. This study is a qualitative research with a true experimental design employing a post-test only control group design. The study used male Wistar rats (Rattus norvegicus) totaling 24 individuals, which were divided into 4 groups: a control group and 3 treatment groups. Each group consisted of 6 rats induced with formaldehyde at concentrations of 20 ppm, 30 ppm, and 40 ppm. The Wistar rats will be terminated, and retro-orbital blood sampling will be conducted to count the various types of leukocytes in order to determine whether there are differences in leukocyte type counts among rats induced with formaldehyde. Data analysis will employ the Kruskal-Wallis test. The results of this study showed that there was a statistically significant difference (p <0.05) between formaldehyde and the total count of leukocyte types. In this study, it was found that there is a decrease in the total count of leukocyte types in the formaldehyde-exposed groups at 40 ppm, 30 ppm, and 20 ppm when compared to the control group.

References

Al-Sarraj, A., & Al-Habity, A. (2013). Effect of formaldehyde vapor on the blood constituents of male rabbits. In Iraqi Journal of Veterinary Sciences (Vol. 27, Issue 1). http://www.vetmedmosul.org/

Asare-Donkor, N. K., Kusi Appiah, J., Torve, V., Voegborlo, R. B., & Adimado, A. A. (2020). Formaldehyde Exposure and Its Potential Health Risk in Some Beauty Salons in Kumasi Metropolis. Journal of Toxicology, 2020. https://doi.org/10.1155/2020/8875167

Gerberich, H. R., & Seaman, G. C. (2013). Kirk-Othmer Encyclopedia of Chemical Technology. In Kirk-Othmer Encyclopedia of Chemical Technology.

Hall, J. E., & Guyton, A. C. (2014). Guyton and Hall Textbook of Medical Physiology (12th Edition). Elsevier.

Jia, X., Jia, Q., Zhang, Z., Gao, W., Zhang, X., Niu, Y., Meng, T., Feng, B., Duan, H., Ye, M., Dai, Y., Jia, Z., & Zheng, Y. (2014). Effects of formaldehyde on lymphocyte subsets and cytokines in the peripheral blood of exposed workers. PLoS ONE, 9(8). https://doi.org/10.1371/journal.pone.0104069

Kang, D. S., Kim, H. S., Jung, J. H., Lee, C. M., Ahn, Y. S., & Seo, Y. R. (2021). Formaldehyde exposure and leukemia risk: a comprehensive review and network-based toxicogenomic approach. In Genes and Environment (Vol. 43, Issue 1). BioMed Central Ltd. https://doi.org/10.1186/s41021-021-00183-5

Kuo, H. W., Jian, G. J., Chen, C. L., Liu, C. S., & Lai, J. S. (1997). White blood cell count as an indicator of formaldehyde exposure. Bulletin of Environmental Contamination and Toxicology, 59(2), 261–267. https://doi.org/10.1007/s001289900473

Lyapina, M., Zhelezova, G., Petrova, E., & Boev, M. (2004). Flow cytometric determination of neutrophil respiratory burst activity in workers exposed to formaldehyde. International Archives of Occupational and Environmental Health, 77(5), 335–340. https://doi.org/10.1007/s00420-004-0516-3

Martínez-Castro, J., Reyes-Cadena, S., & Felipe-Riverón, E. (2014). Leukocytes Detection, Classification and Counting in Smears of Peripheral Blood (Vol. 35, Issue 1).

Nielsen, G. D., Larsen, S. T., & Wolkoff, P. (2013). Recent trend in risk assessment of formaldehyde exposures from indoor air. In Archives of Toxicology (Vol. 87, Issue 1, pp. 73–98). https://doi.org/10.1007/s00204-012-0975-3

Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci, V., Squadrito, F., Altavilla, D., & Bitto, A. (2017). Oxidative Stress: Harms and Benefits for Human Health. Oxidative Medicine and Cellular Longevity, 2017, 1–13. https://doi.org/10.1155/2017/8416763

Ramos, C. de O., Nardeli, C. R., Campos, K. K. D., Pena, K. B., Machado, D. F., Bandeira, A. C. B., Costa, G. de P., Talvani, A., & Bezerra, F. S. (2017). The exposure to formaldehyde causes renal dysfunction, inflammation and redox imbalance in rats. Experimental and Toxicologic Pathology, 69(6), 367–372. https://doi.org/10.1016/j.etp.2017.02.008

van der Laan, L., Cardenas, A., Vermeulen, R., Fadadu, R. P., Hubbard, A. E., Phillips, R. V., Zhang, L., Breeze, C., Hu, W., Wen, C., Huang, Y., Tang, X., Smith, M. T., Rothman, N., & Lan, Q. (2022). Epigenetic aging biomarkers and occupational exposure to benzene, trichloroethylene and formaldehyde. Environment International, 158. https://doi.org/10.1016/j.envint.2021.106871

Ye, X., Yan, W., Xie, H., Zhao, M., & Ying, C. (2005). Cytogenetic analysis of nasal mucosa cells and lymphocytes from high-level long-term formaldehyde exposed workers and low-level short-term exposed waiters. Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 588(1), 22–27. https://doi.org/10.1016/j.mrgentox.2005.08.005

Zhang, L., Tang, X., Rothman, N., Vermeulen, R., Ji, Z., Shen, M., Qiu, C., Guo, W., Liu, S., Reiss, B., Freeman, L. B., Ge, Y., Hubbard, A. E., Hua, M., Blair, A., Galvan, N., Ruan, X., Alter, B. P., Xin, K. X., … Lan, Q. (2010). Occupational Exposure to Formaldehyde, Hematotoxicity, and Leukemia-Specific Chromosome Changes in Cultured Myeloid Progenitor Cells. Cancer Epidemiology, Biomarkers & Prevention, 19(1), 80–88. https://doi.org/10.1158/1055-9965.EPI-09-0762

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.

The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.

Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.

Most read articles by the same author(s)

Ni Kadek Lioni, Anak Agung Ayu Niti Wedayani, Dyah Purnaning, Mukaddam Alaydrus, The Knowledge Level of Elderly in Karang Taliwang Village and the Incidence of Low Back Pain in 2025 , Jurnal Biologi Tropis: Vol. 25 No. 1 (2025): Januari - Maret
Gabriella Chrisanta Dwi Ananda Putri, Anak Agung Ayu Niti Wedayani, Eka Arie Yuliyani, Sekar Partiwi, EGF Levels in Rattus norvegicus Induced with 40 ppm Formaldehyde for 1 Month in Relation to Nasopharyngeal Cancer , Jurnal Biologi Tropis: Vol. 25 No. 2 (2025): April-Juni
Muhammad Raksa Nagara Dijaya, Anak Agung Ayu Niti Wedayani, Pujiarohman Pujiarohman, Azizatul Adni, Incidence of Insomnia in Geriatric Patients at the Taliwang Health Center in 2025 , Jurnal Biologi Tropis: Vol. 25 No. 2 (2025): April-Juni
Keysha Angelica Sinaga, Maz Isa Ansyori Arsatt, Basuki Rahmat, Anak Agung Ayu Niti Wedayani, The Knowledge Level of Hypertension Stage 2 Patients at Harapan Keluarga Hospital Regarding Complications in the Form of Aortic Aneurysm , Jurnal Biologi Tropis: Vol. 25 No. 2 (2025): April-Juni
Fania Octivani, Maz Isa Ansyori Arsatt, Akhada Maulana, Anak Agung Ayu Niti Wedayani, Relationship Between Patients with Hypertension Stage 2 and Chronic Kidney Disease (CKD) at Harapan Keluarga Hospital , Jurnal Biologi Tropis: Vol. 25 No. 2 (2025): April-Juni
Aulia Putri Cahyani, Akhada Maulana, Maz Isa Ansyori Arsatt, Anak Agung Ayu Niti Wedayani, The Knowledge Level of Elderly in Puskesmas Taliwang and the Incidence of Chronic Kidney Disease in 2024 , Jurnal Biologi Tropis: Vol. 25 No. 2 (2025): April-Juni
Yulia Dian Theresia, Maz Isa Ansyori, Akhada Maulana, Anak Agung Ayu Niti Wedayani , Incidence of Cefixime Resistance in Patients with Chronic Kidney Disease at Harapan Keluarga Hospital , Jurnal Biologi Tropis: Vol. 25 No. 2 (2025): April-Juni
M. Arya Rifqi Ilham, Ilsa Hunaifi, Bayu Tirta Dirja, Effect of MLC901 on Red Cell Distribution Width (RDW) in Acute Ischemic Stroke: Literature Review , Jurnal Biologi Tropis: Vol. 24 No. 2 (2024): April - Juni
Natalia Tantowi, Anak Agung Ayu Niti Wedayani, Pujiarohman Pujiarohman, Azizatul Adni, I Gusti Ketut Artha Jaya Kusuma, The Incidence of Depression in Elderly Patients with Hypertension Stage 2 in Karang Taliwang , Jurnal Biologi Tropis: Vol. 25 No. 2 (2025): April-Juni
Ni Putu Ayu Nindya Dewi Cahya Putri, Anak Agung Ayu Niti Wedayani, Ima Arum Lestarini, Abdul Qadar Punagi, Nova Audrey Luetta Pieter, Muhammad Husni Cangara, Novia Andansari Putri Restuningdyah, The Effect of Feeding Non-Antioxidant Feed on The Degree of Dysplasia in Formaldehyde-Induced Wistar Rats , Jurnal Biologi Tropis: Vol. 23 No. 1 (2023): Special Issue

1 2 > >>

The Relationship Between The Total Count of Leukocyte Types and Formahldehyde Induction in Wistar Rats

Authors

DOI:

Published:

Issue:

Keywords:

Articles

Downloads

How to Cite

Downloads

Metrics

Abstract

References

License

Most read articles by the same author(s)

Similar Articles

Menu

Manuscript Template

visitor

Keywords

Mitra Kerja sama

Open Access Journals

Contact Us

Information

Share & Follow