White Blood Cell Differentiation Based on Toll-Like Receptor 4 (TLR4) Gene Polymorphism in Local Indonesian Chickens
Authors
Maskur Maskur , Rahma Jan , Tapaul Rozi , L. Kasip , Muhammad MuhsininDOI:
10.29303/jbt.v24i1.6518Published:
2024-02-12Issue:
Vol. 24 No. 1 (2024): Januari - MaretKeywords:
blood profile, local chickens, marker-assisted-selection, TLR4 gene.Articles
Downloads
How to Cite
Downloads
Metrics
Abstract
Toll-like Receptors (TLRs) genes are a group of genes that transcribe TLRs proteins which act as receptors on the surface of phagocytic cells to recognize molecular patterns of pathogenic microbial components. This research aims to identify the variability of the TLR4 gene and its relationship with blood profiles in local Indonesian chickens. The samples used to identify TLR4 gene diversity in local Indonesian chickens were 135 samples. Research methods include DNA extraction, PCR amplification, RFLP analysis, and genotype determination. The genotyping results in the research were associated with a complete blood profile so that a genotype was obtained that could be used as a marker for resistance traits based on the blood profile. The results of this study showed that the TLR4 gene was polymorphic with three genotype types and an allele frequency of more than 0.01 in each KUB, Sensi, and Broiler CP 707 chicken. The frequency of the GG genotype is the highest compared to the AG and AA genotypes. White blood cell concentration values showed variations in the three genotypes of TLR4 gene and were still within normal standards, although statistically the differences were not significant.
References
Abasht B., M.G. Kaiser, J. van der Poel, & S.J. Lamont (2009). Genetic lines differ in Toll-like receptor gene expression in spleens of chicks inoculated with Salmonella enterica serovar Enteritidis. Poultry Science 88 :744–749. doi: 10.3382/ps.2008-00419
Akashi, S., Nagai, Y., Ogata, H., Oikawa, M., Fukase, K., Kusumoto, S., et al. (2001). Human MD-2 confers on mouse toll-like receptor 4 species-specific lipopolysaccharide recognition. Int. Immunol. 13, 1595–1599. doi: 10.1093/ intimm/13.12.1595.
Akira S, & Takeda K. (2004). Toll-like receptor signalling. Nat Rev Immunol. 4(7):499–511
Allendorf FW, Luikar G, & Aitken SN. (2013). Conservation and the genetics of population. 2nd ed. Oxford (UK): Wiley-Blackwell.
Beaumont, C., Protais, J., Pitel, F., Leveque, G., Malo, D., & Lantier, F. (2003). Effect of two candidate genes on the salmonella carrier state in fowl. Poult. Sci. 82, 721–726. doi: 10.1093/ps/82.5.721
Bishop SC, & Woolliams JA. (2014). Genomics and disease resistance studies in livestock. Livest Sci.166(1): 190-198. DOI: 10.1016/j.livsci.2014.04.034
Campbell, T.W., & C.K. Ellis (2013). Avian and Exotic Animal Hematology and Cytology. 3th Edition. John Wiley & Sons,
Coles Brian H. (2006). Essentials of Avian Medicine and Surgery, Third edition. Blackwell Publishing: www.blackwellpublishing.com
Davis, A.K., D.L. Maney, & J.C. Maerz. (2008). The use of leukocyte profiles to measure stress in vertebrates: a review for ecologists. Funct. Ecol., 22 (2008), pp. 760-772. https://doi.org/10.1111/j.1365-2435.2008.01467.x
Emertcan A, Ozturk F, & Gunduz K. (2011). Toll-like receptors and skin. Journal of the European Academy of Dermatology and Venereology 11:1-7. doi: 10.1016/j.yadr.2008.09.004
Guyton ac., & Hall JE. (2006). textbook of medical physiology / John Hall. – 12th ed. Saunders Elsevier. http://avaxho.me/blogs/ChrisRedfield.
Jie, H., Lian, L., Qu, L., Zheng, J., Hou, Z., Xu, G., et al. (2013). Differential expression of toll-like receptor genes in lymphoid tissues between Marek's disease virus-infected and noninfected chickens. Poult. Sci. 92, 645–654. doi: 10.3382/ps.2012-02747
Kabelitz D. (2007). Expression and function of Tolllike receptors in T lymphocytes. Curr Opin Immunol. 19: 39-45. DOI: 10.1016/j.coi.2006.11.007
Kim Hyo Jin, Hyemin Kim, Jeong Hyung Lee & Cheol Hwangbo (2023). Toll-like receptor 4 (TLR4): new insight immune and aging. BMC.,20:67. Doi.org/10.1186/s12979-023-00383-3
Kogut, M. H., Iqbal, M., He, H., Philbin, V., Kaiser, P., & Smith, A. (2005). Expression and function of toll-like receptors in chicken heterophils. Dev. Comp. Immunol. 29, 791–807. doi: 10.1016/j.dci.2005.02.002
Kramer J, Malek M, & Lamont SJ. (2003). Association of twelve candidate gene polymorphisms and response to challenge with Salmonella enteritidis in poultry. Anim Genet. 34(5): 339-348.
Kumar K.P., Alyce J. Nicholls & Connie H. Y. Wong. (2018). Partners in crime: neutrophils and monocytes/macrophages in inflammation and disease. Cell and Tissue Research (2018) 371:551–565. doi: 10.1007/s00441-017-2753-2
Lamont SJ, Kaiser MG, & Liu W. (2002). Candidate genes for resistance to Salmonella enteritidis colonization in chickens as detected in a novel genetic cross. Vet Immunol Immunopathol. 87(3-4): 423-428. DOI: 10.1016/s0165-2427(02)00064-8
Lokapirnasari WP, Yulianto AB, Legowo D, & Agustono (2016). The effect of spirulina as feed additive to myocardial necrosis and leukocyte of chicken with Avian Influenza (H5N1) virus infection. Proced Chem. 18(1): 213-217. doi: 10.1016/j.proche.2016.01.033
Maeda Y. (2005). Science and technology for indigenous poultry development in South East Asia. Di dalam: Kumpulan Makalah Utama Seminar Nasional Tentang Unggas Lokal III. 2005 Agustus 25; Semarang, Indonesia. Semarang (ID): Universitas Diponegoro. hlm 1-22.
Mortaz E, Alipoor SD, Adcock IM, Mumby S & Koenderman L. (2018). Update on Neutrophil Function in Severe Inflammation. Front. Immunol. 9:2171. doi: 10.3389/2018.02171
Nei M, Kumar S. (2000). Molecular Evolution and Phylogenetics. New York (US): Oxford University Press.
Palsson-McDermott, E. & O’Neill, L. (2004) Signal transduction by the lipopolysaccharide receptor, Toll-like receptor- 4. Immunology, 113, 153-162.DOI: 10.1111/j.1365-2567.2004.01976.x
Pollack, C., J.W. Carpenter, & N. Antinoff (2005). In: Birds, 3rd Ed. J Carpenter ed. Exotic Animal Formulary. Elsevier Saunders, St. Louis, MO
Rehman MS, Rehman Su, Yousaf W, Hassan F, Ahmad W, Liu Q & Pan H. (2021). The Potential of Toll-Like Receptors to Modulate Avian Immune System: Exploring the Effects of Genetic Variants and Phytonutrients. Front. Genet. 12:671235. doi: 10.3389/2021.671235
Ruhs EC, Martin LB, & Downs CJ. (2020). The impacts of body mass on immune cell concentrations in birds. Proc. R. Soc. B 287: 20200655. DOI: 10.1098/rspb.2020.0655
Sartika T., Sri Sulandari, & M.S.A. Zein (2011). Selection of Mx gene genotype as genetic marker for Avian Influenza resistance in Indonesian native chicken. BMC Proceedings 2011, 5(Suppl 4): S37. http://www.biomedcentral.com/1753-6561/5/S4/S37
Sastradipradja D, Sri Hartini SS, Reviany W, Tonny U, Achmad M, Hamdani N, Regina S, & Razak H. (1989). Penuntun Praktikum Fisiologi Veteriner. Bogor (ID): Pusat Antar Universitas Ilmu Hayat Percetakan IPB. Hlm. 13-17.
Sohail M.U., Hume M.E., Byrd J.A., Nisbet D.J., Ijaz A., Sohail A., Shabbir M.Z., & Rehman H. (2012). Effect of supplementation of prebiotic mannan-oligosaccharides and probiotic mixture on growth performance of broilers subjected to chronic heat stress. Poult. Sci. 91:2235–2240. doi: 10.3382/ps.2012-02182
Sohail M.U., Ijaz A., Yousaf M.S., Ashraf K., Zaneb H., Aleem M., & Rehman H. (2010). Alleviation of cyclic heat stress in broilers by dietary supplementation of mannan-oligosaccharide and lactobacillus-based probiotic: Dynamics of cortisol, thyroid hormones, cholesterol, C-reactive protein, and humoral immunity. Poult. Sci.; 89:1934–1938. doi: 10.3382/ps.2010-00751.
Stabler, J. G., T. W. McCormick, K. C. Powell, & M. H. Kogut, (1994). Avian heterophils and monocytes: phagocytic and bactericidal activities against Salmonella enteritidis. Vet. Microbiol. 38:293–305. DOI: 10.1016/0378-1135(94)90148-1
Sulandari S, Zein MSA, Astuti D, & Sartika (2009). T: Genetic polymorphisms of the chicken antiviral Mx gene in a variety of Indonesian indigenous chicken breeds. J Veteriner 2009, 10(2):50-56.
Tamiru N. Alkie, Alexander Yitbarek, Douglas C. Hodgins, Raveendra R. Kulkarni, Khaled Taha-Abdelaziz & Sharif S. (2019). Development of innate immunity in chicken embryos and newly hatched chicks: a disease control perspective, Avian Pathology, 48:4, 288-310, DOI: 10.1080/03079457.2019.1607966
Tamzil MH, Noor RR, Hardjosworo PS, Manalu W, & Sumantri C. (2013). Acute heat stress exposure on three lines of chickens with different heat shock protein (HSP)-70 genotypes. Int J Poult Sci. 12(5): 264-272. ISSN 1682-8356
Tohidi, R., I. B. Idris, J. M. Panandam, & M. H. Bejo. (2013). The effects of polymorphisms in 7 candidate genes on resistance to Salmonella Enteritidis in native chickens. Poult Sci. 92(4): 900-909. DOI: 10.3382/ps.2012-02797
Virden W.S. & M. T. Kidd. (2009). Physiological stress in broilers: Ramifications on nutrient digestibility and responses. J. Appl. Poult. Res. 18 :338–347 doi: 10.3382/japr.2007-00093
Yalcinkaya, L., T. M. Gonggor, Basalan & E. Erdem. (2008). Mannan oligosaccharides (MOS) from Saccharomyces cerevisiae in broilers: Effects on performance and blood biochemistry. Turk. J. Vet. Anim. Sci., 32(1): 43-48. https://doi.org/10.1016/j.carbpol.2021.118467
Zuoyong Z., Z Wang, L Cao, S Hu, Z Zhang, Bo Qin, Z Guo, & K Nie. (2013). Upregulation of chicken TLR4, TLR15 and MyD88 in heterophils and monocyte-derived macrophages stimulated with Eimeria tenella in vitro. Exp. Parasitol. 133(4):427-33. DOI: 10.1016/j.exppara.2013.01.002
License
Copyright (c) 2024 Maskur Maskur, Rahma Jan, Tapaul Rozi, L. Kasip, Muhammad Muhsinin
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.