Identification of Single Nucleotide Polymorphism (SNP) in Exon 5 of the Prolactin Gene in Native Chickens
DOI:
10.29303/jbt.v25i1.8342Published:
2025-01-06Downloads
Abstract
The prolactin gene (PRL) in chickens is one of the genes that regulates broodiness and egg production in laying hens. Single Nucleotide Polymorphism (SNP) is a form of single nucleotide variation in the DNA base sequence at specific locations in the genome that can influence differences in quantitative traits in livestock. This study aimed to identify SNPs in the prolactin gene of native chickens. The research methods included DNA extraction, PCR amplification, and DNA sequence analysis for genotype determination of individuals. The results revealed four mutation points at positions g.8052 bp T>C, g.7886 T>A, g.7823 A>G, and g.8069 T>C. All mutation points in exon 5 were substitution mutations. Statistical analysis showed that all loci were polymorphic and the population was in Hardy–Weinberg equilibrium.
Keywords:
PRL gene, egg production, native chickens, Marker-Assisted SelectionReferences
Alipanah M, Shojaian K, & Bandani HK. (2011). The polymorphism of prolactin gene in native chicken zabol region. J Anim Vet Adv. 10:619-621.
Bhattacharya TK, Chatterjee RN, Sharma RP, Niranjan M, Rajkumar U, & Reddy BLN. (2011). Polymorphism in the prolactin promoter and its association with growth traits in chickens. Biochem Genet. 49:385-94.
Chen CF, Shiue YL, Yen CJ, Tang PC, Chang HC, & Lee YP. (2007). Laying traits and underlying transcripts, expressed in the hypothalamus and pituitary gland, that were associated with egg production variability in chickens. Theriogenol. 68:1305-1315.
Cui JX, Du HL, Liang Y, Deny XM, Li N, & Zhany XQ. (2006). Association of polymorphism in the promoter region of chicken prolactin with age production. Poult Sci. 85: 26–31.
Erehehuara (2003). Studies on the SNP of the chicken’s prolactingene. Wuhan: Huazhong Agricultural University.
Fadillah A. (2016). Performa produktivitas induk hasil seleksi pada persilangan ayam pelung ras pedaging sentul kampung [skripsi]. Bogor: Institut Pertanian Bogor.
Food and Agriculture Organization of The United Nations (2008). Native chicken genetic resources and production systems in Indonesia. Prepared by Muladno. GCP/RAS/228/GER Working Paper No. 6. Rome (IT): FAO.
Halley C, & Visscher P. (2000). DNA marker and genetic testing in farm animal improvement: Current applications and future prospects. In: Depth Rev Read Inst cell, Anim Popul Biol Univ Edinburgh. Edinburgh (Scotland): University of Edinburgh.
Iskandar S, & Susanti T. (2007). Karakter dan Manfaat Ayam Pelung di Indonesia. Wartazoa. 17 (3): 128-136.
Jiang RS, Xu GY, Zhang XQ, & Yang N. (2005). Association of polymorphisms for prolactin and prolactin receptor genes with broody traits in chickens. Poult Sci. 84:839-845.
Lewin B. (1997). Genes are mutable units. In: Genes VI. New York (US): Oxford University Press. p. 51-70.
Li HF, Shu JT, Du YF, Shan YJ, Chen KW, Zhang XY, Han W, & Xu WJ. (2013). Analysis of the genetic effects of prolactin gene polymorphisms on chicken egg production. Mol Biol Rep. 40:289–294.
Montaldo, H. H. & C. A. M. Herrera. (1998). Use of molecular markers and major genes in genetic improvement of livestock. J. Biotech. 1 : 2.
Muladno (2002). Seputar Teknologi Rekayasa Genetika. Pustaka Wirausaha Muda dan USESE Foundation, Bogor.
Nataamijaya AG. (2000). The native of chiken of Indonesia. Bull Plasma Nutfah. 6(1): 1-6.
Nataamijaya AG. (2010). Pengembangan potensi ayam lokal untuk menunjang peningkatan kesejahteraan petani. J Litbang Pertanian. 29(4): 131-138.
Sartika T, Duryadi D, Mansjoer SS, Saefuddin A, & Martojo H. (2004). Gen promotor prolaktin sebagai penanda pembantu seleksi untuk mengontrol sifat mengeram pada ayam kampung. JITV. 9:239-245.
Sartika T, & Iskandar S. (2007). Mengenal plasma nutfah ayam Indonesia dan pemanfaatannya. Sukabumi (ID): Kepraks.
Sharp PJ, Scanes CG, Williams JB, Harvey S, & Chadwick A. (1979). Variations in concentrations of prolactin, luteinizing hormone, growth hormone and progesterone in the plasma of broody bantams (Gallus domesticus). J. Endocrinol. 80: 51–57.
Sumantri, C., A. Anggraeni, A. Farajallah & D. Perwitasari (2007). Keragaman mikrosatelit DNA sapi perah FH di Balai Pembibitan Ternak Unggul; Baturaden. J. ITV 12: 124-133.
Susanti T, Noor RR, Hardjosworo PS, & Prasetyo LH. (2012). Relationship between prolactin hormone level, molting and duck egg production. J Indonesian Trop Anim Agric. 37:161-167.
Watahiki M, Tanaka M, Masuda N, Sugisaki K, Yamamoto M, Yamakawa M, Nagai J, & Nakashima K. (1989). Primary structure of chicken pituitary prolactin deduced from the cDNA sequence. Conserved and specific amino acid residues in the domains of the prolactins. J Biol Chem. 264:5535-5539.
Williams, J. L. (2005). The use of marker-assisted selection in animal breeding and biotechnology. Rev. Sci. Tech. of. Int. Epiz., 24:379-391.
License
Copyright (c) 2025 Tapaul Rozi, Maskur Maskur, Rahma Jan, Lalu Muhammad Kasip, Muhammad Muhsinin
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.
