The Effect of Feeding Non-Antioxidant Feed on The Degree of Dysplasia in Formaldehyde-Induced Wistar Rats

Ni Putu Ayu Nindya Dewi Cahya Putri; Anak Agung Ayu Niti  Wedayani; Ima Arum  Lestarini; Abdul Qadar  Punagi; Nova Audrey Luetta  Pieter; Muhammad Husni  Cangara; Novia Andansari Putri  Restuningdyah

doi:10.29303/jbt.v23i1.6091

The Effect of Feeding Non-Antioxidant Feed on The Degree of Dysplasia in Formaldehyde-Induced Wistar Rats

Authors

Ni Putu Ayu Nindya Dewi Cahya Putri , Anak Agung Ayu Niti Wedayani , Ima Arum Lestarini , Abdul Qadar Punagi , Nova Audrey Luetta Pieter , Muhammad Husni Cangara , Novia Andansari Putri Restuningdyah

DOI:

10.29303/jbt.v23i1.6091

Published:

2023-12-06

Issue:

Vol. 23 No. 1 (2023): Special Issue

Keywords:

Antioxidant, dysplasia, feed, formaldehyde, nasopharynx.

Articles

Downloads

pdf

How to Cite

Putri, N. P. A. N. D. C., Wedayani, A. A. A. N. ., Lestarini, I. A. ., Punagi, A. Q. ., Pieter, N. A. L. ., Cangara, M. H. ., & Restuningdyah, N. A. P. . (2023). The Effect of Feeding Non-Antioxidant Feed on The Degree of Dysplasia in Formaldehyde-Induced Wistar Rats. Jurnal Biologi Tropis, 23(1), 380–389. https://doi.org/10.29303/jbt.v23i1.6091

Download Citation

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Abstract

Nasopharyngeal dysplasia is a condition of abnormal cellular growth that occurs due to the failure of nasopharyngeal epithelial cells in carrying out cellular adaptation. This condition can occur due to the continuous inhalation of toxic compounds, such as formaldehyde, which has genotoxic and cytotoxic effects that can induce cellular damage. One of the compound activity associated with reducing risk of dysplasia is antioxidant activity. Therefore, it is important to know whether the presence of antioxidant activity affects the occurrence of dysplasia. This study is an experimental study with a post-test only control group design which aims to determine the effect of Non-Antioxidant Feed on the occurrence of nasopharyngeal dysplasia. Testing of the antioxidant activity in feed was carried out using the DPPH assay. There were two groups of experimental animals including the treatment group (fed with Non-Antioxidant Feed) and the control group (fed with SB 11). All animals in each group were induced with 40 ppm formaldehyde by inhalation for 8 weeks and then euthanized using chloroform inhalation for nasopharyngeal tissue extraction. The results of the histopathological examination of the two groups were then statistically tested using the Mann-Whitney U test and obtained a value of P<0.05 (P=0.006), which means that there are effect of feeding Non-Antioxidants Feed on the degree of dysplasia in formaldehyde-induced wistar rats. The results can be associated with the ingredients contained in the feed given, differences in genetic polymorphisms of each subjects, and effect of body weight variations of the rats caused by randomization.

References

Andersen, C. J. (2015). Bioactive egg components and inflammation. Nutrients, 7(9), 7889–7913. DOI: https://doi.org/10.3390/nu7095372

Arista, M. (2013). Aktivitas Antioksidan Ekstrak Etanol 80% Dan 96% Daun Katuk (Sauropus androgynus (L.) Merr. Jurnal Ilmiah Mahasiswa Universitas Surabaya, 2(2).

Bbosa, G. S., Kitya, D., Lubega, A., Ogwal-Okeng, J., Anokbonggo, W. W., & Kyegombe, D. B. (2013). Review of the Biological and Health Effects of Aflatoxins on Body Organs and Body Systems. In Aflatoxins - Recent Advances and Future Prospects (Vol. 12). InTech. DOI: https://doi.org/http://dx.doi.org/10.5772/51201

Birnbaum, L. S., Anisimov, V. N., United Nations Environment Programme., International Labour Organisation., World Health Organization., & International Program on Chemical Safety. (1993). Principles for evaluating chemical effects on the aged population. World Health Organization.

Boysen, M., Zadig, E., Digernes, V., Abeler, V., & Reith, A. (1990). Nasal mucosa in workers exposed to formaldehyde: a pilot study. In British Journal of Industrial Medicine (Vol. 47).

Doi, K., & Uetsuka, K. (2011). Mechanism of Mycotoxin-Induced Neurotoxicity through Oxidative Stress-Associated Pathways. International Journal of Molecular Sciences, 12. DOI: https://doi.org/10.3390/ijms12085213

Duthie, S. J., Duthie, G. G., Russell, W. R., Kyle, J. A. M., Macdiarmid, J. I., Rungapamestry, V., Stephen, S., Megias-Baeza, C., Kaniewska, J. J., Shaw, L., Milne, L., Bremner, D., Ross, K., Morrice, P., Pirie, L. P., Horgan, G., & Bestwick, C. S. (2017). Effect of increasing fruit and vegetable intake by dietary intervention on nutritional biomarkers and attitudes to dietary change: a randomised trial. European Journal of Nutrition, 57(5), 1855–1872. DOI: ttps://doi.org/10.1007/s00394-017-1469-0

Fischer, A. H., Jacobson, K. A., Rose, J., & Zeller, R. (2008). Hematoxylin and eosin staining of tissue and cell sections. CSH Protocol.

Ghasemi, A., Jeddi, S., & Kashfi, K. (2021). The Laboratory Rat: Age And Body Weight Matter. In Excli Journal (Vol. 20, pp. 1431–1445). Leibniz Research Centre for Working Environment and Human Factors. DOI: https://doi.org/10.17179/excli2021-4072

Golalipour, M. J., Azarhoush, R., Ghafari, S., Gharravi, A. M., Fazeli, S. A., & Davarian, A. (2007). Formaldehyde exposure induces histopathological and morphometric changes in the rat testis. Folia Morphol, 66(3), 167–171. URL: www.fm.viamedica.pl

Johnson, D. E., Burtness, B., Leemans, C. R., Lui, V. W. Y., Bauman, J. E., & Grandis, J. R. (2020). Head and Neck Squamous Cell Carcinoma. Nature Reviews, 6(92). DOI: https://doi.org/10.1038/s41572-020-00224-3

Kamal, N., Ilowefah, M. A., Hilles, A. R., Anua, N. A., Awin, T., Alshwyeh, H. A., Aldosary, S. K., Jambocus, N. G. S., Alosaimi, A. A., Rahman, A., Mahmood, S., & Mediani, A. (2022). Genesis and Mechanism of Some Cancer Types and an Overview on the Role of Diet and Nutrition in Cancer Prevention. Molecules, 1–27.

Lee, J. H., & Paik, H. D. (2019). Anticancer and immunomodulatory activity of egg proteins and peptides: a review. Poultry Science, 98(12), 6505–6516. DOI: https://doi.org/10.3382/ps/pez381

Leso, V., Macrini, M. C., Russo, F., & Iavicoli, R. (2020). Formaldehyde Exposure and Epigenetic Effects: A Systematic Review. Multidisciplinary Digital Publishing Institute (MDPI), 10.

Marjoni, M., & Zulfisa, A. (2017). Antioxidant Activity of Methanol Extract/Fractions of Senggani Leaves (Melastoma candidum D. Don). Pharmaceutica Analytica Acta, 08(08). DOI: https://doi.org/10.4172/2153-2435.1000557

Maronpot, R. R., Miller, R. A., Clarke, W. J., Westerberg, R. B., Decker, J. R., & Moss, O. R. (1986). Toxicity Of Formaldehyde Vapor In B6c3f1 Mice Exposed For 13 Weeks. In Toxicology (Vol. 41).

Mulia, K., Hasan, A. E. Z., & Suryani. (2016). Total Phenolic, Anticancer and Antioxidant Activity of Ethanol Extract of Piper retrofractum Vahl from Pamekasan and Karang Asem. Current Biochemistry, 3(2), 80–90. URL: http://biokimia.ipb.ac.id

Munteanu, I. G., & Apetrei, C. (2021). Analytical methods used in determining antioxidant activity: A review. International Journal of Molecular Sciences, 22(7). DOI: https://doi.org/10.3390/ijms22073380

Nielsen, G. D., Larsen, S. T., & Wolkoff, P. (2013). Recent trend in risk assessment of formaldehyde exposures from indoor air. In Archives of Toxicology (Vol. 87, Issue 1, pp. 73–98). DOI: https://doi.org/10.1007/s00204-012-0975-3

Peraica, M., Radic, B., Lucic, A., & Pavlovic, & M. (1999). Toxic effects of mycotoxins in humans. Bulletin of the World Health Organization, 77.

Poljsak, B., Šuput, D., & Milisav, I. (2013). Achieving the balance between ROS and antioxidants: When to use the synthetic antioxidants. Oxidative Medicine and Cellular Longevity. DOI: https://doi.org/10.1155/2013/956792

PT. Charoen Pokphand Indonesia Tbk. (n.d.). SB 11. PT. Charoen Pokphand Indonesia Tbk.

Putri, N. P. A. N. D. C., Wedayani, A. A. A. N., Punagi, A. Q., Pieter, N. A. L., Cangara, M. H., Kadriyan, H., Susilawati, N. K., Hidayat, L. H., Fauzan, M., Makbul, I. H., Wiguna, I. M. D., Abdiman, I. M. T., Nugraha, G. R. H., Yassa, I. W. R. W., Kusuma, I. A. J., Malaka, R., & Pratiwi, S. (2023). Pakan Hewan Laboratorium Tanpa Aktivitas Antioksidan sebagai Pakan Hewan Untuk Pembuatan Hewan Model pada Studi Kesehatan (Patent No. 000476100). URL: www.tcpdf.org

Rytsyk, O., Soroka, Y., Shepet, I., Vivchar, Z., Andriichuk, I., Lykhatskyi, P., Fira, L., Nebesna, Z., Kramar, S., & Lisnychuk, N. (2020). Experimental Evaluation of the Effectiveness of Resveratrol as an Antioxidant in Colon Cancer Prevention. SAGE Journal, 15(6), 1–10.

Sayin, V. I., Ibrahim, M. X., Larsson, E., Nilsson, J. A., Lindahl, P., & Bergo, M. O. (2014). Antioxidants Accelerate Lung Cancer Progression in Mice. Science Translational Medicine, 6(221). URL: www.ScienceTranslationalMedicine.org

Setiawan, V., Phangestu, S., Grace Soetikno, A., Arianti, A., & Kohar, I. (2021). Rapid screening analysis of antioxidant activities in green tea products using DPPH and FRAP. Pharmaceutical Journal of Indonesia, 7(1), 9–14. DOI: https://doi.org/10.21776/ub.pji.2021.007.01.2

Shabeer, S., Asad, S., Jamal, A., & Ali, A. (2022). Aflatoxin Contamination, Its Impact and Management Strategies: An Updated Review. In Toxins (Vol. 14, Issue 5). MDPI. DOI: https://doi.org/10.3390/toxins14050307

Sung, H., Ferlay, J., Siegel, R. L., Laversanne, M., Soerjomataram, I., Jemal, A., & Bray, F. (2021). Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA: A Cancer Journal for Clinicians, 71(3), 209–249. DOI: https://doi.org/10.3322/caac.21660

Susilawati, N. K., Yasa, I. W. P. S., Suardana, W., Maliawan, S., Jawi, I. M., Romdhoni, A. C., Kadriyan, H., Putra, I. W. G. A. E., & Arijana, I. G. K. N. (2022). Purple Sweet Potato Reduces Malondialdehyde and TNF-a, Increases p53, and Protects Histopathological Appearance in Formaldehyde-induced Nasopharyngeal Carcinoma Rats. Indonesian Biomedical Journal, 14(2), 211–217. DOI: https://doi.org/10.18585/inabj.v14i2.1906

Swenberg, J. A., Moeller, B. C., Lu, K., Rager, J. E., Fry, R. C., & Starr, T. B. (2013). Formaldehyde Carcinogenicity Research: 30 Years and Counting for Mode of Action, Epidemiology, and Cancer Risk Assessment. Toxicologic Pathology, 41(2), 181–189. DOI: https://doi.org/10.1177/0192623312466459

Tesfaye, S., Hamba, N., Gerbi, A., & Negeri, Z. (2020). Endocrinology & Metabolic Syndrome Oxidative Stress and Carcinogenic Effect of Formaldehyde Exposure: Systematic Review & Analysis. Endocrinology & Metabolic Syndrome, 9(6). DOI: https://doi.org/10.35248/2161-1017.20.9.319

Vaishali Aggarwal, Tuli, H. S., Varol, A., Thakral, F., Yerer, M. B., Sak, K., Varol, M., Jain, A., Khan, Md. A., & Sethi, G. (2019). Role of Reactive Oxygen Species in Cancer Progression: Molecular Mechanisms and Recent Advancements. Biomolecules, 4(1), 88–100.

Wangikar, P. B., Dwivedi, P., Sinha, N., Sharma, A. K., & Telang, A. G. (2005). Teratogenic effects in rabbits of simultaneous exposure to ochratoxin A and aflatoxin B1 with special reference to microscopic effects. Toxicology, 215(1–2), 37–47. DOI: https://doi.org/10.1016/j.tox.2005.06.022

Wedayani, A. A. A. N., Punagi, A. Q., Pieter, N. A., Cangara, M. H., Kadriyan, H., Susilawati, N. K., Nurhidayati, Putri, N. A., Hidayat, L. H., Makbul, I. H., Fauzan, M., Abdiman, I. M. T., Wiguna, I. M. D., & Kusuma, I. A. J. (2023). Kandang Tikus Ekonomis Sebagai Media Induksi Displasia UntukStudi Kesehatan (Patent No. 000473934). URL: www.tcpdf.org

White, M. C., Holman, D. M., Boehm, J. E., Peipins, L. A., Grossman, M., & Henley, S. J. (2015). Age and Cancer Risk. American Journal of Preventive Medicine, 46.

World Health Organization. (2000). Hazardous Chemicals in Human and Environmental Health.

World Health Organization. (2022, February 3). Cancer. World Health Organization. URL: https://www.who.int/news-room/fact-sheets/detail/cancer

Young, B., O’Dowd, G., & Woodford, P. (2017). Wheater’s Functional Histology A Text and Colour Atlas. In Neuroprosthetics: Theory and Practice: Second Edition. DOI: https://doi.org/10.1142/9789813207158_0004

Zhang, V. X., Sze, K. M. F., Chan, L. K., Ho, D. W. H., Tsui, Y. M., Chiu, Y. T., Lee, E., Husain, A., Huang, H., Tian, L., Wong, C. C. L., & Ng, I. O. L. (2021). Antioxidant supplements promote tumor formation and growth and confer drug resistance in hepatocellular carcinoma by reducing intracellular ROS and induction of TMBIM1. Cell and Bioscience, 11(1). DOI: https://doi.org/10.1186/s13578-021-00731-0

License

Copyright (c) 2023 Ni Putu Ayu Nindya Dewi Cahya Putri, Anak Agung Ayu Niti Wedayani, Ima Arum Lestarini, Abdul Qadar Punagi, Nova Audrey Luetta Pieter, Muhammad Husni Cangara, Novia Andansari Putri Restuningdyah

This work is licensed under a Creative Commons Attribution 4.0 International License.

Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.

The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.

Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.

The Effect of Feeding Non-Antioxidant Feed on The Degree of Dysplasia in Formaldehyde-Induced Wistar Rats

Authors

DOI:

Published:

Issue:

Keywords:

Articles

Downloads

How to Cite

Downloads

Metrics

Abstract

References

License

Most read articles by the same author(s)

Similar Articles

Menu

Manuscript Template

visitor

Keywords

Mitra Kerja sama

Open Access Journals

Contact Us

Information

Share & Follow