Qualitative Analysis of Partial 16S rRNA Amplicon of Mitochondrial Gene of Stingless Bees in Pesawaran
DOI:
10.29303/jbt.v23i1.6470Published:
2023-12-26Downloads
Abstract
Pesawaran is one of the areas that has the potential to cultivate stingless bees as producers of honey and propolis. This study was aimed at qualitatively analysing partial amplicons of the 16S rRNA gene from individuals found at the research site. The research was performed from April to September 2023 in an explorative approach. Based on the exploration that has been done in Harapan Jaya Village, Way Ratai District, Pesawaran Regency, individual stingless bees were obtained from 5 different colonies. Individual samples of stingless bees were further extracted and amplified by commercial kits. Each individual from 5 different colonies has successfully obtained its DNA amplicons. Based on qualitative analysis using 1% agarose gel, the size of the partial gene ranged from 400bp to 500bp. This result is in accordance with the data contained in the genbank, where the 16S rRNA gene size is more than 500bp. Therefore, it has been concluded that the size of the partial amplicons of the 16S rRNA gene of individual stingless bees from the 5 colonies that were obtained is actually in range of the size of the 16S rRNA gene of stingless bees that have been recorded in the genbank.
Keywords:
16S rRNA, partial amplicon, stingless bee.References
Arias, M.C., Brito, R.M., Francisco, F.O., Moretto, G., de Oliveira, F.F., Silvestre, D., Sheppard, W.S. (2006). Molecular markers as a tool for population and evolutionary studies of stingless bees. Apidologie 37(2) 259 – 274. DOI: https://doi.org/10.1051/apido:2006021
Augusteyn, R.C., Vrensen, G., Willekens, B. (2008). The effect of paraformaldehyde fixation and PBS storage on the water content of the human lens. Molecular Vision: Biology and Genetics in Vision Research, 14: 90 – 94.
Balai Penelitian dan Pengembangan Teknologi Hasil Hutan Bukan Kayu. (2018). Panduan Singkat Budidaya dan Breeding Lebah Trigona spp. Lombok: Litbang Kementerian Lingkungan Hidup dan Kehutanan.
Fatoni, A. 2008. Pengaruh Propolis Trigona spp. Asal Bukittinggi terhadap Beberapa Bakteri Usus Halus Sapi dan Penelusuran Komponen Aktifnya. [Disertasi]. Institut Pertanian Bogor, Bogor.
Galtier, N., Nabholz B., Glémin S., and Hurst G. D. (2009). Mitochondrial DNA as a marker of molecular diversity: a reappraisal. Mol. Ecol. 18: 4541 – 4550. DOI: 10.1111/j.1365-294X.2009.04380.x
Gissi, C., Iannelli F., and Pesole G. (2008). Evolution of the mitochondrial genome of Metazoa as exemplified by comparison of congeneric species. Heredity (Edinb). 101: 301 – 320. DOI: 10.1038/hdy.2008.62
Hage, G.M., Selan, Y.N., Amalo, F. (2014). Studi Anatomi Lampung Kelelawar Buah (Pteropus vampyrus) dengan Pewarnaan Histokimia Periodic Acid Shiff (PAS). Jurnal Kajian Veteriner, 2(2): 193 – 201. DOI: https://doi.org/10.35508/jkv.v2i2.1004
Harahap, A.S. (2017). Ujia Kualitas dan Kuantitas DNA Beberapa Populasi Pohon Kapur Seumatera. Journal of Animal Science and Agronomi Panca Budi 2(2): 1 – 6.
Heinsohn, N.K., Niedl, R.R., Anielski, A., Lisdat, F., Beta, C. (2022). Elektrophoretic µPAD for Purification and Analysis of DNA Samples. Biosensors, 12(2): 62. DOI: https://doi.org/10.3390/bios12020062
Herwina, H., Salmah, S., Jasmi, Yaherwandi, Mairawita, Janra, M.N., Rusdimansyah, Christy, B.Y., Sari, D.A., Putri, G. (2021). West Sumatran Stingless Bees (Hymenoptera: Apidae: Meliponini): What can be told from its Local Distribution. IOP Conf. Series: Earth and Environmental Science 757 (2021) 01208. DOI: 10.1088/1755-1315/757/1/012084.
Herwina, H., Janra, M.N., Salmah, S., Mairawita, Jasmi. (2022). Analisis Cepat terhadap Budidaya Galo-Galo (Apidae: Meliponini) di Desa Suntur, Kecamatan Barangin, Kota Sawahlunto. Aksiologiya: Jurnal Pengabdian kepada Masyarakat, 6(3): 388 – 399. DOI: https://doi.org/10.30651/aks.v6i3.5168.
Juli. (2021). Budi Daya Madu Trigona di Lampung Timur: Primadona dalam Masa Pandemi COVID-19.
Kelly, N., Farisya, M.S.N., Kumara, T.K., Marcela, P. (2014). Species diversity and external nest characteristics of stingless bees in meliponiculture. Per. J. Trop. Agric. Sc. 37(3): 293 – 298.
Lamerkabel, J.S.A., Siahaya, V.G., Saepuloh, W., Lsatriyanto, A., Junus, M., Erwan, Batoro, J., Jaya, F., Masyithoh, D. (2021). Karakteristik Morfologi dan Morfometruk Lebah Madu Tak Bersengat (Apidae; Melliponinae) pada Koloni di Daerah Pesisir Pulau Ambon. Jurnal Budidaya Pertanian, 17(1): 28 – 35. DOI: 10.30598/jbdp.2021.17.1.28
Lee, P.Y., Costumbrado, J., Hsu, C., Kim, Y.H. (2012). Agarose Gel Electrophoresis for the Separation of DNA Fragments. Journal of Visualized Experiment. DOI: 10.3791/3923
Permatasari, N., Priyambodo, Sari, R.P.K., Fauziah, L.K. (2023). Pelatihan Penambahan Tanaman Sumber Nektar pada Masyarakat Pembudidaya Lebah Tanpa Sengat di Desa Harapan Jaya, Kecamatan Way Ratai, Kabupaten Pesawaran. Jurnal Pengabdian Dharma Wacana, 3(4): 379 – 385. DOI: https://doi.org/10.37295/jpdw.v3i4.379
Perry, C., Chung, J.Y., Ylaya, K., Choi, C.H., Simpson, A., Matsumoto, K.T., Smith, W.A., Hewitt, S.M. (2016). A Buffered Alcohol-Based Fixative for Histomorphologic and Molecular Applications. Journal of Histochemistry, 64(7): 425 – 440. DOI: 10.1369/0022155416649579
Pesaresi, M., Buscemi, L., Alessandrini, F., Cecati, M., Tagliabracci, A. (2003). Qualitative and quantitative analysis of DNA recovered from fingerprints. International Congress Series 1239: 947 – 951.
Prasetyo dan Aryono. (2020). Berkunjung ke Suhita Bee Farm di Bandar Lampung.
Pratama, M.N., Agus, A., Umami, N., Agussalim, Purwanto, H. (2023). Morphometric and molecular identification, domestication, and potentials of stingless bees (Apidae: Meliponini) in Mount Halimun Salak National Park, West Java, Indonesia. Biodiversitas 24(11): 6107 – 6118. DOI: 10.13057/biodiv/d241132
Priyambodo, Putri, C.R.P., Rustiati, E.L., Kurniawati, Y., Zulkarnain, D., Pratiwi, D.N., Arsan, Z., Giyono, G., Mustikawati, G., Pertiwi, B.R., Sukartmoko, S., Srihanto, E.A., Saswiyanti, E. (2023). Amplification of The GAPDH Gene from The Urine eDNA of Sumatran Rhino in Sumatran Rhino Sanctuary, Way Kambas National Park. Jurnal Biologi Tropis, 23(3): 110 – 114. DOI: 10.29303/jbt.v23i3.4989
Purwanto, H., dan Trianto, M. Species Description, Morphometric Measurement, and Molecular Identification of Stingless Bees (Hymenoptera: Apidae: Meliponini) in Meliponiculture Industry in West Java Province, Indonesia. Serangga 2021, 26(1): 13-33.
Rachmawati, R.D., Agus, A., Umami, N., Agussalim, Purwanto, H. (2022). Diversity, distribution, and nest characteristics of stingless bees (Hymenoptera: Meliponini) in Baluran National Park, East Java, Indonesia. Biodiversitas 23 (8): 3890 – 3901. DOI: https://doi.org/10.13057/biodiv/d210633
Rustiati, E.L., Priyambodo, Novianasari, T., Pratiwi, D.N., Srihanto, E.A., Angeliya, L. (2019). Comparative Study on DNA Extraction’s Qualitative Anaysis of Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park. Journal of Physics: Conference Series 1338 012029. DOI: 10.1088/1742-6596/1338/1/012029
Sambrook, J., dan Russel, D.W. (2001). Molecular Cloning-A Laboratory Manual. New. York: Cold Spring Harbor Laboratory Press.
Sari, P.J. (2015). Studi Awal: Histoteknik Perfusi PBS-Formalin dan Gambaran Histologi Organ Hepar, Pankreas, dan Ginjal Tikus Strain Sprague Dawley. Skripsi. Jakarta: UIN Syarif Hidayatullah.
Sarma, B., Rout, J., Bhatnagar, K. (2014). Nitella mirabilis from Assam: Morphology and Molecular Characterization using RAPD markers. VEGETOS 27(1): 97 – 102.
Sidik, M., Lestari, I.A., Sa’uddah, L.D., Yani, A.A., Priyambodo (a). 2022. Karakterisasi Beebread dari Tujuh Spesies Lebah Tanpa Sengat (Stingless Bee) Berdasarkan Keanekaragaman Pollen yang Dikumpulkannya. Bio Sains: Jurnal Ilmiah Biologi, 2 (1): 30 – 38. DOI: https://doi.org/10.6084/m9.figshare.23564679
Sidik, M., Lestari, I.A., Sa’uddah, L.D., Yani, A.A., Priyambodo (b). 2022. Pelatihan Budidaya Lebah Tanpa Sengat di Desa Harapan Jaya Kecamatan Way Ratai Kabupaten Pesawaran. MITRAWARGA: Jurnal Pengabdian Kepada Masyarakat, 1(1): 11 – 16. DOI: https://doi.org/10.23960/jmw.v1i1.9
Slaa, E.J., Chaves, L.A.S., Malagodi-Braga, K.S., Hofstede, F.E. (2006). Stingless bees in applied pollination: practice and perspectives. Apidologie 37(2): 293 – 315. DOI: https://doi.org/10.1051/apido:2006022.
Stricland, M., Johnson, M.E., Broandbent, J.R. (2001). Qualitative and quantitative analysis of proteins and peptides in milk products by capillary electrophoresis. Electrophoresis 22(8). DOI: 10.1002/1522-2683(200105)22:8<1510::AID-ELPS1510>3.0.CO;2-4.
Suhandy, D., Yulia, M., Kusumiyati. 2022. Rapid authentication of stingless bees (Heterotrigona itama) honey by UV spectroscopy and hierarchical cluster analysis. IOP Conf. Series: Earth and Environmental Science 1024 (2022) 012064. DOI: 10.1088/1755-1315/1024/1/01206.
Thummajitsakul, S., Silprasit, K., Klinbunga, S., Sittipraneed, S. (2013). The partial mitochondrial sequence of the Old World stingless bee, Tetragonula pagdeni. J. Genet. 92, 299–303.
Trianto, M., dan Purwanto, H. (2020a). Morphological characteristics and morphometrics of Stingless Bess (Hymenoptera: Meliponini) in Yogyakarta, Indonesia. Biodiversitas, 21(6): 2619 – 2628. DOI: 10.13057/biodiv/d210633.
Trianto, M., dan Purwanto, H. (2020b). Molecular Phylogeny of Stingless Bees in the Special Region of Yogyakarta Revealed Using Partial 16S rRNA Mitochondrial Gene. Buletin Peternakan 44(4): 186 – 193. DOI: 10.21059/buletinpeternak.v44i4.55539.
Trianto, M., Arisuryanti, T., Purwanto, H., Ubaidillah, R. (2023). Updated Species Check-list of the Indonesian Stingless Bees (Hymenoptera, Apidae, Apinae, Meliponini). Journal of Tropical Biodiversity and Biotechnology, 8(2): 1 – 24. DOI: 10.22146/jtbb.77160
Wang, C., Zhao, M., Wang, S., Xu, H., Yang, Y., Liu, L., Feng, Y. (2021). The Complete Mitochondrial Genome of Lepidotrigona flavibasis (Hymenoptera: Meliponini) and High Gene Rearrangement in Lepidotrigona Mitogenomes. Journal of Insect Science 21(3): 10 DOI: 10.1093/jisesa/ieab038
Wijayanti, G.E., Setyawan, P., Kurniawati, D.I. (2017). A simple paraffin embedded protocol for fish egg, embryo, and larvae. Scripta Biologica, 4(2): 85 – 89. DOI: https://doi.org/10.20884/1.SB.2017.4.2.420.
License
Copyright (c) 2024 Priyambodo Priyambodo, Elly Lestari Rustiati, Dian Neli Pratiwi, Alvin Wiwiet Susanto, Aulia Imtitsal, Aditya Fahrezi, Muhammad Febriansyah, Andriyani Wijaya Kusuma, Eko Agus Srihanto, Enny Saswiyanti, Mahfud Sidik, Lousanja D. Sa'uddah, Indah Ayu Lestari, Ani Andri Yani, Viki Ramadhan
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.
