Difference Results Bacteria Numbers with Variations Temperature and Check Time in The Elderly Urine Suspect Urinary Tract Infections (UTI)
Authors
Risma Dyah Septiani , Yusianti SilvianiDOI:
10.29303/jbt.v24i2.6723Published:
2024-05-30Issue:
Vol. 24 No. 2 (2024): April - JuniKeywords:
Elderly, germ count, mayo examination, urinary tract infection.Articles
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Abstract
Delays in examination have the potential to result in differences in the results of the number of bacteria in the examined specimens plus storage of specimens at non-optimal temperatures. The difference in the number of bacteria can be known by examining the Total Plate Count (TPC) in urine. The purpose of this study was to determine the differences in the results of germ numbers in terms of variations in examination delays and urine storage temperatures of elderly suspects with urinary tract infections (UTI). The type of research conducted was experimental analytic with the subject of 4 elderly urine in Kratonan Village, Surakarta with criteria sampling technique. Research data were obtained through questionnaires and examination of total plate numbers with mayo technique examination, where the results were multiplied by a conversion factor of 102 CFU/ml. The results were analyzed by SPSS statistical test using Two Way ANOVA which obtained a significant value of 0.915 (p>0.05). Then continued with SPSS data analysis using Tukey Pos Hoc aims to see the real difference of each variation. There is no difference in the germ count results with temperature variations and time delays in the urine of elderly suspected urinary tract infection (UTI) patients.
References
Afraghassani, S., Sejahtera, & Ambar Wulan, S. (2019). Glutic. Rancang Bangun Alat Pendeteksi Glukosa Urin Berbasis Teknologi Sensor Serat Optik Untuk Diagnosis Dini Diabetes. Glutic. Rancang Bangun Alat Pendeteksiglukosa Urinberbasis Teknologi Sensorserat Optik Untuk Diagnosis Dini Diabetes, 6(1), 1–12. http://journal.unismuh.ac.id/
Akbar, F., Darmiati, D., Arfan, F., & Putri, A. A. Z. (2021). Pelatihan dan Pendampingan Kader Posyandu Lansia di Kecamatan Wonomulyo. Jurnal Abdidas, 2(2), 392–397. https://doi.org/10.31004/abdidas.v2i2.282
Anisah, & Rahayu, T. (2015). Media Alternatif untuk Pertumbuhan Bakteri Menggunakan Sumber Karbohidrat yang Berbeda Alternative Media For Bacterial Growth Using Different Source of Carbohidrats. Seminar Nasional XII Pendidikan Biologi FKIP UNS, 10(1), 855–860.
Aziza, V., & Rini, C. S. (2019). The Effect of Temperature and Storage Time of Urine in Patients with Urinary Tract Infections ( UTI ) on The Number of Bacteria Pengaruh Suhu dan Lama Penyimpanan Urine Pada Pasien Infeksi Saluran Kemih ( ISK ) Terhadap Jumlah Bakteri. Artike Ilmiah, 1–8.
Biaggini, K., Barbey, C., Borrel, V., Feuilloley, M., Déchelotte, P., & Connil, N. (2015). The pathogenic potential of Pseudomonas fluorescens MFN1032 on enterocytes can be modulated by serotonin, substance P and epinephrine. Archives of Microbiology, 197(8), 983–990. https://doi.org/10.1007/s00203-015-1135-y
Brooks, G.F., Stephen A.M., Karen C.C., Timothy A.M., and Janet S.B. 2013. Jawetz, Melnick, and Adelberg’s Medical Microbiology Twenty-Sixth Edition. New York: The McGraw-Hill Companies, Inc.
Fitri, I. (2019). Pengaruh Variasi Lama Penundaan Pemeriksaan Terhadap Enumerasi Bakteri Pada Urin Penderita Infeksi Saluran Kemih (Isk). Jurnal Biologi Dan Pembelajarannya (JB&P), 6(2), 12–14. https://doi.org/10.29407/jbp.v6i2.14793
Fitri, I., Rizki Aziz, Z. M., & Widyawati, D. I. (2021). Effect of Check Delay Time Difference on Enumerating Bacteria in Patients with Urinary Tract Infection. Jurnal Biologi Tropis, 21(3), 720–725. https://doi.org/10.29303/jbt.v21i3.2860
Huang, C. (2020). Extensively drug-resistant Alcaligenes faecalis infection. BMC Infectious Diseases, 20(1), 1–11. https://doi.org/10.1186/s12879-020-05557-8
Kadarsih, A. (2017). Hitung Jumlah Bakteri Urin Tersangka Infeksi Saluran Kemih Pada Penyimpanan Suhu Ruang dan Lemari Es. Analisis Biologi, 01(233), 19–24. https://jurnal.yayasanbaktiasih-bdg.co.id/index.php/jab/article/download/132/112
Nafisah, S., & Mubarak, Z. (2023). Gambaran Kejadian Infeksi Saluran Kemih (ISK) pada Ibu Hamil Di Desa Singorojo Kendal. Jurnal Nursing Update, 14(2), 477–482. https://stikes-nhm.e-journal.id/NU/index
Nantika, R. catur, Noviar, G., Marliana, N., & Nurhayati, B. (2017). Pengaruh Suhu dan Waktu Penyimpanan Urine Terhadap Titer Status Sekretor. Riset Kesehatan Poltekes Bandung, 11(2), 204–210.
Paerunan, A., Sakung, J., & Hamidah. (2018). Analisis Kandungan Bakteri Pada Daging Sapi dan Ayam yang Dijual di Pasar Sentral Daya Kota Makassar. Jurnal Kolaboratif Sains, 1(1), 1–11.
Paluseri, A., Rosyid, S. Z., Asriani, A., Muslimin, L., & Guntur, M. (2022). Evaluasi Penggunaan Antibiotik Pada Pasien Rawat Jalan Infeksi Saluran Kemih Di RS Pendidikan Universitas Hasanuddin. Wal’afiat Hospital Journal, 3(2), 104–114. https://doi.org/10.33096/whj.v3i2.90
Pardede, S. O. (2018). Infeksi pada Ginjal dan Saluran Kemih Anak: Manifestasi Klinis dan Tata Laksana. Sari Pediatri, 19(6), 364. https://doi.org/10.14238/sp19.6.2018.364-74
Rame Hau, E. E., & Rohyati, E. (2018). Pengaruh Kondisi Dan Lama Penyimpanan Pada Suhu Ruang Dan Refrigerator Terhadap Angka Total Plate Count (Tpc) Sampel Sei Babi Dari 4 Toko Di Kota Kupang. Partner, 23(2), 860. https://doi.org/10.35726/jp.v23i2.328
Respati, N. Y., Yulianti, E., & Rahmawati, A. (2017). Optimasi Suhu Dan Ph Media Pertumbuhan Bakteri Pelarut Fosfat Dari Isolat Bakteri Termofilik. Kingdom (The Journal of Biological Studies), 6(7), 423–430. https://doi.org/10.21831/kingdom.v6i7.7864
Rolfe, M. D., Rice, C. J., Lucchini, S., Pin, C., Thompson, A., Cameron, A. D. S., Alston, M., Stringer, M. F., Betts, R. P., Baranyi, J., Peck, M. W., & Hinton, J. C. D. (2012). Lag phase is a distinct growth phase that prepares bacteria for exponential growth and involves transient metal accumulation. Journal of Bacteriology, 194(3), 686–701. https://doi.org/10.1128/JB.06112-11
Setiawati, M.R; Suryatmana, P; Herdiyantiri, D; Ilmiyati, Z. (2014). Karakteristik Pertumbuhan Dan Waktu Generasi Isolat Azotobacter Sp. Dan Bakteri Endofitik Asal Ekosistem Lahan Sawah. Jurnal Agroekotek, 6(1), 12–20.
Setyati, W. A., Martani, E., Triyanto, T., & Zainuddin, M. (2015). Growth kinetics and Protease Activity 36K Isolates Derived from Mangrove Ecosystem Sediment, Karimunjawa, Jepara (Kinetika Pertumbuhan dan Aktivitas Protease Isolat 36k Berasal dari Sedimen Ekosistem Mangrove, Karimunjawa, Jepara). ILMU KELAUTAN: Indonesian Journal of Marine Sciences, 20(3), 163. https://doi.org/10.14710/ik.ijms.20.3.163-169
Sulaimah, R., Renshaleksamana, E., Zaetun, S., & Jiwintarum, Y. (2022). Viabilitas Bakteri Pada Spesimen Klinis Penderita Infeksi Saluran Kemih Menggunakan Bio-porter Sebagai Wadah Transport. Journal of Indonesia Laboratory Technology of Student (JILTS), 1(1), 22–30.
Suriani, S., Soemarno, & Suharjo. (2013). Pengaruh Suhu dan pH terhadap Laju pertumbuhan Lima Isolat Bakteri Anggota Genus Pseudomonas yang diisolasi dari Ekosistem Sungai Tercemar Deterjen di sekitar Kampus Universitas Brawijaya. Indonesian Journal of Environment and Suistainable Development, 3(2), 58–62.
Vebriyanti, E., Arief, I. I., Salundik, S., & Dewi, P. (2022). Karakteristik Mikroorganisme, pH dan Unsur Hara Urin Sapi Perah di Daerah Bogor, Jawa Barat. Jurnal Agripet, 22(2), 133–140. https://doi.org/10.17969/agripet.v22i2.19844
Widiyastuti, S. F., & Soleha, T. U. (2023). Faktor Faktor Yang Mempengaruhi Terjadinya Infeksi Saluran Kemih. Fakultas Kedokteran Universitas Lampung, 13, 1069–1073. https://journalofmedula.com/index.php/medula/article/view/825/670
Yashir, M. and Apriani. (2019). Variasi Bakteri Pada Penderita Infeksi Saluran Kemih (ISK). Jurnal Media Kesehatan. 12(2): 102–109. https://doi.org/10.33088/jmk.v12i2.441.
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