Screening of Endosymbion Fungus Potential on The Stem of Avicennia sp. from Shore of Bandar Lampung City as an Antibacterial
DOI:
10.29303/jbt.v24i2.7141Published:
2024-06-22Downloads
Abstract
Mangroves scattered in the shore of Lampung have considerable potential to be used as medicines and antibiotics, because the distribution of various mangroves on almost every shore of Lampung is overgrown with various mangrove plants. Mangroves have a symbiotic relationship with microbes, so that mangrove endosymbion microbes generally have the same bioactive compounds as their hosts. Mangrove Avicennia sp. is one of the mangrove species that has the ability to be antibacterial. Mangrove endosymbion microbes can also have the ability to inhibit antibacterial pathogens that cause disease. Diseases whose cases are found in the community, one of which is caused by Staphylococcus aureus bacteria and Escherichia coli. The purpose of this study is to screen the potential isolate of the endosymbion fungus Avicennia sp. as an antibacterial against S.aureus and E.coli bacteria. Isolation results of Avicennia sp. 12 isolates of endosymbion fungi were obtained that have inhibitory activity against S. aureus and E. coli. and 5 isolates (WBJ-R01, WBJ-R04, WBJ-R05, WBJ-R09, and WBJ-R12) that had the best inhibitory zone activity. Extracts from the five endosymbion fungi isolates have inhibitory zones in both pathogenic bacteria. It is proven that the five extracts at the highest concentration are in a strong inhibition zone. WBJ-R01, WBJ-R04, WBJ-R05, WBJ-R09, and WBJ-R12 isolates had inhibition zones of 11.4 mm, 11 mm, 11.73 mm, 12.3 mm, and 13.23 mm, against S.aureus bacteria respectively. and against E.coli bacteria 14.03 mm, 10.3 mm, 13.13 mm, 9.73 mm, and 6.5 mm.
Keywords:
Antibacteria, E. coli, mangrove Avicennia sp., S. aureus.References
Ayunda R. (2015). Isolasi, Seleksi, dan Uji Aktivitas Antibakteri dari Kapang En-dofit Daun Parijoto (Medinilla speciosa Blume) terhadap Staphylococcus aureus, Bacillus subtilis, Escherichia coli, dan Shigella dysenteriae (Skripsi). UIN Syarif Hidayatullah. Jakarta. 108 hlm.
Cowan, M. M. (1999). Plant products as antimicrobial agents. Clinical micro- biology reviews. 12(4):564-582. DOI: 10.1128/cmr.12.4.564
Darminto, Ali, A., & Dini, I. (2009). Indentifikasi senyawa metabolit sekunder potensial menghambat pertumbuhan bakteri aeromonas hydrophyla dari kulit batang tumbuhan Aveccennia sp. Chemica: Jurnal Ilmiah Kimia dan Pendidikan Kimia. 10(2):92-98.
Denatri AH, Maisaroh DS, Kartika AGD, Susanti O, & Munir M. (2023). Antibacterial activities of the extracts of sponge Agelas cervicornis agains bacteria Staphylococcus aureus. Journal of Marine Resources & Coastal Management. 4(2):01-06. DOI: 10.29080/mrcm.v4i2.1592
Esati, N.K., Jawa La, E.O., & Lestari, G.A.D. (2022). Uji aktivitas antioksidan ekstrak etanol daun rosemary (Rosemarinus officinalis L.) dengan metode DPPH dan FRAP serta pengaplikasiannya sebagai zat aktif dalam losion. Jurnal Sains dan Kesehatan. 4(4):363-369. DOI: 10.25026/jsk.v4i4.1129
Ganjar, G.I., & Rohman, A. (2007). Kimia Farmasi Analisis. Pustaka Pelajar. Yogyakarta. 545 hlm.
Maier, R. M., & Pepper, I. L. (2015). Bacterial Growth. In Environmental Microbiology: Third Edition (pp. 37-56). Elsevier Inc.. https://doi.org/10.1016/B978-0-12-394626-3.00003-X
Muharni, M., Fitrya, F., & Farida, S. (2017). Uji aktivitas antibakteri ekstrak etanol tanaman obat Suku Musi di Kabupaten Musi Banyuasin, Sumatera Selatan. Jurnal Kefarmasian Indonesia. 7(2):27-135. DOI: 10.22435/jki.v7i2.6070.127-135
Mulyadi, M., Wuryanti., & Ria, P. S. (2013). Konsentrasi hambat minimum (KHM) kadar sampel alang-alang (Imperata cylindrica) dalam etanol me-lalui metode difusi cakram. Chem Info. 1(1): 35-42. DOI: 10.14710/jksa.20.3.130-135
Nawea, Y., Mangindaan, R., & Bara, R. (2017). Uji antibakteri jamur endofit dari tumbuhan mangrove Sonneratia alba yang tumbuh di Perairan Pantai Tanawangko. Jurnal Pesisir dan Laut Tropis, 5(1): 24-45. DOI: 10.35800/jplt.5.1.2017.14993
Nurama DF, Maesaroh DS, Munir M, Kartika AGD, Susanti O, & Joesidawati MI. (2023). Antibacterial potential marine sponge extract and bacteria symbionts Callyspongia vaginalis from Kendit Waters Against the bacteria Vibrio harveyi. IOP Conf. Series: Earth and Environmental Science. 1251: 012028. DOI:10.1088/1755-1315/1251/1/012028
Nurasiah, E. S. (2010). Pengoptimuman Ekstraksi Andrografolida dari Sambiloto dengan Rancangan Fraksional Faktorial (Skripsi). Institut Pertanian Bogor. Bogor.
Posangi, J., & Bara, R. A. (2014). Analisis aktivitas dari jamur endofit yang ter- dapat dalam tumbuhan bakau Avicennia marina di Tasik Ria Minahasa. Jurnal Pesisir dan Laut Tropis. 2(1):30-38. DOI: 10.35800/jplt.2.1.2014.7345
Prihatiningtias W. (2005). Senyawa bioaktif jamur endofit akar kuning (Fibraurea Hloroleucac Miers) sebagai senyawa antimikroba. (Thesis). Pascasarjana Universitas Gajah Mada. Yogyakarta: 235 hlm.
Ravimannan, N., Arulanantham, R., Pathmanathan, S., & Niranjan, K. (2014). Alternative culture media for fungal growth using different formulation of protein sources. Annals of Biological Research. 5(1):36-39.
Septiani, S., Dewi, E. N., & Wijayanti, I. (2017). Aktivitas antibakteri ekstrak lamun (Cymodocea rotundata) terhadap bakteri Staphylococcus aureus dan Escherichia coli. Saintek Perikanan: Indonesian Journal of Fisheries Science and Technology. 13(1):1-6. DOI: https://doi.org/10.14710/ijfst.13.1.1-6
Rossiana, N., Miranti, M., Rahmawati, R., Setyobudi, R. H., Nuringtyas, T. R., & Adinurani, P. G. (2016). Antibacterial Activities of Endophytic Fungi from Mangrove Plants Rhizophora apiculata L. and Bruguiera gymnorrhiza (L.) Lamk. on Salmonella typhi. AIP Conference Proceedings, 1744(1), 1-6. DOI: https://doi.org/10.1063/1.4953514.
Susanti O, Putra PM, Putri A. (2023). The Antibacterial Study from Endosymbiont Fungals of Mangroves (Avicennia sp.) in Lampung Waters against Staphylococcus aureus and Escherichia coli. Jurnal Marshela (Marine and Fisheries Tropical Applied Journal). 1(2):47-54. DOI: 10.25181/marshela.v1i2.3177
Strobel GA. (2003). Endophytes as sources of bioactive products microbes. Microbes and infection. 5(6):535-544. DOI: 10.1016/s1286-4579(03)00073-x
Trianto A, Nirwani, Susanti O, Maesaroh DS, Radjasa OK. (2019). The bioactivity of bacterium and fungi living associate with the sponge Reniera sp. Against multidrug-resistant Staphylococcus aureus and Escherichia coli. Biodiversitas. 20(8):2302-2307. DOI: 10.13057/biodiv/d200827
Wahyuni WT, Herdiyanto, Rafi M. (2017). Metode Ekstraksi dan Pemisahan Optimum Untuk Isolasi Xantorizol dari Temulawak (Curcuma xanthorrhiza). Jurnal Jamu Indonesia. 2(2):43-50. DOI: https://doi.org/10.29244/jji.v2i2.31
Wijaya, H., Novitasari., & Jubaidah, S. (2018). Perbandingan metode ekstraksi terhadap rendemen ekstrak daun rambai laut (Sonneratia caseolaris L. Engl). Jurnal Ilmiah Manuntung. 4(1):79-83. DOI: https://doi.org/10.51352/jim.v4i1.148
License
Copyright (c) 2024 Oktora Susanti, Esti Harpeni, Eko Efendi, Nisa Karima, Aslam Muamar
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.
