Isolation of Cellulolytic Bacteria from Oyster Mushroom Baglog Compost

Sandra Cahya Kurnia; Lolita Endang Susilowati; Dori Kusuma Jaya

doi:10.29303/jbt.v25i2.7770

Isolation of Cellulolytic Bacteria from Oyster Mushroom Baglog Compost

Authors

Sandra Cahya Kurnia , Lolita Endang Susilowati , Dori Kusuma Jaya

DOI:

10.29303/jbt.v25i2.7770

Published:

2025-04-09

Issue:

Vol. 25 No. 2 (2025): April-Juni

Keywords:

Oyster mushroom baglog compost, cellulolytic bacteria, isolation, cellulose decomposer, Carboxymethyl Cellulose, Congo Red test.

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Kurnia, S. C., Lolita Endang Susilowati, & Jaya, D. K. (2025). Isolation of Cellulolytic Bacteria from Oyster Mushroom Baglog Compost. Jurnal Biologi Tropis, 25(2), 1126–1132. https://doi.org/10.29303/jbt.v25i2.7770

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Abstract

Oyster mushroom baglog compost is an organic waste rich in cellulose, making it a potential source for the isolation of cellulolytic bacteria. This research aims to isolate cellulolytic bacteria that function as decomposers in Baglog compost. Oyster mushroom baglog compost was used as a substrate, and bacteria were isolated using Nutrient Agar (NA) media and Carboxymethyl Cellulose (CMC) media. Qualitative testing method was done with congo red test. The results of the study successfully characterized 3 (three) potential cellulolytic bacterial isolates characterized by a cellulolytic index of more than 1 (1-1.75) and have the following characteristics: one isolate is milky white with a large round shape, one isolate is white with a large choppy shape, and one isolate is yellow with a medium round shape. These findings indicate that the three isolates have potential as cellulose decomposers and can be utilized in organic waste treatment applications or compost production.

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Isolation of Cellulolytic Bacteria from Oyster Mushroom Baglog Compost

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