Isolation of Cellulolytic Bacteria from Oyster Mushroom Baglog Compost

Sandra Cahya Kurnia; Lolita Endang Susilowati; Dori Kusuma Jaya

doi:10.29303/jbt.v24i4.7770

Isolation of Cellulolytic Bacteria from Oyster Mushroom Baglog Compost

Authors

Sandra Cahya Kurnia , Lolita Endang Susilowati , Dori Kusuma Jaya

DOI:

10.29303/jbt.v24i4.7770

Published:

2024-12-09

Issue:

Vol. 24 No. 4 (2024): Oktober - Desember

Keywords:

Oyster mushroom baglog compost, cellulolytic bacteria, isolation, cellulose decomposer, Carboxymethyl Cellulose, Congo Red test.

Articles

Downloads

pdf

How to Cite

Kurnia, S. C., Lolita Endang Susilowati, & Jaya, D. K. (2024). Isolation of Cellulolytic Bacteria from Oyster Mushroom Baglog Compost. Jurnal Biologi Tropis, 24(4), 1134–1140. https://doi.org/10.29303/jbt.v24i4.7770

Download Citation

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Abstract

Oyster mushroom baglog compost is an organic waste rich in cellulose, making it a potential source for the isolation of cellulolytic bacteria. This research aims to isolate cellulolytic bacteria that function as decomposers in Baglog compost. Oyster mushroom baglog compost was used as a substrate, and bacteria were isolated using Nutrient Agar (NA) media and Carboxymethyl Cellulose (CMC) media. Qualitative testing method was done with congo red test. The results of the study successfully characterized 3 (three) potential cellulolytic bacterial isolates characterized by a cellulolytic index of more than 1 (1-1.75) and have the following characteristics: one isolate is milky white with a large round shape, one isolate is white with a large choppy shape, and one isolate is yellow with a medium round shape. These findings indicate that the three isolates have potential as cellulose decomposers and can be utilized in organic waste treatment applications or compost production.

References

Ali, U., & Wajdi, M. F. (2017). Pemanfaatan Bakteri Selulolitik Sekum Kelinci Dengan Aras Konsentrasi Koloni Dan Waktu Inkubasi Untuk Fermentasi Limbah Agroindustri Lokal Dalam Pakan Kelinci. Sains Peternakan, 13(2), 94.Https://Doi.Org/10.20961/Sainspet.V12i2.4780

Andriany, A., Fahruddin, F., & Abdullah, A. (2018). Pengaruh Jenis Bioaktivator Terhadap Laju Dekomposisi Seresah Daun Jati Tectona Grandis L.F., di Wilayah Kampus Unhas Tamalanrea. Bioma : Jurnal Biologi Makassar, 3(2), 31–42. Https://Doi.Org/10.20956/Bioma.V3i2.5820

Apun, K., Jong, B. C., & Salleh, M. A. (2000). Screening And Isolation Of A Cellulolytic And Amylolytic Bacillus From Sago Pith Waste. Journal Of General And Applied Microbiology, 46(5), 263–267. Https://Doi.Org/10.2323/Jgam.46.263

Arif, A, & Hasyim A, H. (2023). Isolasi Dan Karakteristik Bakteri Pendegradasi Selulosa Dari Serasah Daun Tebu (Saccarum Officinarum). Jurnal Bioshell, 12(1), 41–48. Https://Doi.Org/10.56013/Bio.V12i1.2052

Berlanga, M. (2014). Brock Biology Of. May, 12–14.

Dewi Chusniasih, Erma Suryanti, & Erina Safitri. (2023). Isolasi Dan Uji Aktivitas Selulolitik Bakteri Asal Limbah Bagas. Jurnal Ilmu Pertanian Indonesia, 28(3), 386–395. Https://Doi.Org/10.18343/Jipi.28.3.386

Gupta, P., Samant, K., & Sahu, A. (2012). Isolation Of Cellulose-Degrading Bacteria And Determination Of Their Cellulolytic Potential. International Journal Of Microbiology, 2012. Https://Doi.Org/10.1155/2012/578925

Kamelia, M., Anggoro, B. S., & Novitasari, D. (2018). Retracted: Isolasi Dan Seleksi Enzimatis Bakteri Selulolitik Dari Limbah Media Tanam Jamur Tiram Putih (Pleurotus Ostreatus) Berbahan Serbuk Gergaji Kayu Karet (Hevea Brasiliensismuell. Arg). Biosfer: Jurnal Tadris Biologi, 9(2), 225–237. Https://Doi.Org/10.24042/Biosfer.V9i2.2382

Kurniawan, A., Sari, S., Asriani, E., Sambah, A., Kurniawan, A., & Prihanto, A. (2019). Culturable Cellulolytic Bacteria From Mangrove With Anadara Granosa Cultivation In Sukal, West Bangka. January. Https://Doi.Org/10.2991/Icoma-18.2019.28

Kurniawan, C. A., & Gusmawartati, G. (2022). Uji Isolat Bakteri Selulolitik Sebagai Dekomposer Pada Dekomposisi Tandan Kosong Kelapa Sawit. Agrotek: Jurnal Ilmiah Ilmu Pertanian, 5(1), 55–62. Https://Doi.Org/10.33096/Agrotek.V5i1.159

Lee, N., & Schmidt, T. M. (2016). Microbial_Communities_Functional_Versus.Pdf.

Lynd, L. R., Weimer, P. J., Zyl, W. H. Van, & Isak, S. (2002). Microbial Cellulose Utilization : Fundamentals And Biotechnology Microbial Cellulose Utilization : Fundamentals And Biotechnology Downloaded From Http://Mmbr.Asm.Org/ On February 6 , 2013 By Indian Institute Of Technology Madras. Microbiology And Molecular Biology Reviews, 66(3), 506–577. Https://Doi.Org/10.1128/Mmbr.66.3.506

Meryandini, A., Widosari, W., Maranatha, B., Sunarti, T. C., Rachmania, N., & Satria, H. (2010). Isolasi Bakteri Selulolitik Dan Karakterisasi Enzimnya. Makara Journal Of Science, 13(1). Https://Doi.Org/10.7454/Mss.V13i1.369

Murtiyaningsih, H., & Hazmi, M. (2017). Isolasi Dan Uji Aktivitas Enzim Selulase Pada Bakteri Selulolitik Asal Tanah Sampah. Agritrop, 15(2), 293–308. Http://Jurnal.Unmuhjember.Ac.Id/

Prasetyo, M. T., Kusnarta, I. G. M., Susilowati, L. E., & Mahrup. (2023). The Quality Of Compost Made From A Mixture Of Oyster Mushroom Baglog Waste And Cow Manure With The Addition Of Dekomposer Of Promi, Ma-11, And Bpf. Jurnal Biologi Tropis, 23(2), 464–471. Https://Doi.Org/10.29303/Jbt.V23i2.4874

Rudiansyah, D., Rahmawati, & Rafdinal. (2017). Eksplorasi Bakteri Selulolitik Dari Tanah Hutan Mangrove. Protobiont, 6(3), 255–262.

Sazci, A., Erenler, K., & Radford, A. (1986). Detection Of Cellulolytic Fungi By Using Congo Red As An Indicator: A Comparative Study With The

Dinitrosalicyclic Acid Reagent Method. Journal Of Applied Bacteriology, 61(6), 559–562. Https://Doi.Org/10.1111/J.1365-2672.1986.Tb01729.X

Sukmawati, D., P., & Warisaura, D., A. (2022). Pengaruh Perbandingan Komposisi Antara Limbah Baglog Dengan Kotoran Sapi Menggunakan Em-4. Serambi Engineering, 7(3), 3609–3616.

Supriyatna, A., Rohimah, I., Suryani, Y., & Sa’adah, S. (2012). Isolation And Identification Of Cellulolytic Bacteria From Waste Organic Vegetables And Fruits For Role In Making Material Biogas. Jurnal Istek, 6(2), 10–20.

Susilowati, L. E., Arifin, Z., Silawibawa, I. P., R. Sutriono, & Mahrup. (2022). Edukasi Pengolahan Limbah Baglog Jamur Tiram Menjadi Pupuk Organik Diperkaya Bakteri Pelarut Fosfat Pada Petani Muda Milenial Di Desa Narmada Kabupaten Lombok Barat. Jurnal Pengabdian Magister Pendidikan Ipa, 5(4), 46–53. Https://Doi.Org/10.29303/Jpmpi.V5i4.2370

Susilowati, L. E., Mahrup, M., Arifin, Z., & Sutriono, R. (2023). Effectiveness Of Bio Activator Of Phosphate Solubilizing Bacteria Consortium On Composting Bag-Log Waste Incorporated With Cow Dung. Jurnal Penelitian Pendidikan Ipa, 9(2), 788–795. Https://Doi.Org/10.29303/Jppipa.V9i2.2755

Weimer, P. J. (2022). Degradation Of Cellulose And Hemicellulose By Ruminal Microorganisms. Microorganisms, 10(12). Https://Doi.Org/10.3390/Microorganisms10122345

Yogyaswari, S. A., Rukmi, M. G. I., & Raharjo, B. (2016). Ekplorasi Bakteri Selulolitik Dari Cairan Rumen Sapi Peranakan Fries Holland (Pfh) Dan Limousine Peranakan Ongole (Limpo). Jurnal Biologi, 5(4), 70–80.

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.

The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.

Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.

Isolation of Cellulolytic Bacteria from Oyster Mushroom Baglog Compost

Authors

DOI:

Published:

Issue:

Keywords:

Articles

Downloads

How to Cite

Downloads

Metrics

Abstract

References

License

Most read articles by the same author(s)

Similar Articles

Menu

Manuscript Template

visitor

Keywords

Mitra Kerja sama

Open Access Journals

Contact Us

Information

Share & Follow