Literature Review: The Effect of Butterfly Pea Flower Kombucha Fermentation as Antibacterial Agent
Authors
Zerati Hijriadina , Buchary Rahman Ash Shiddiqi , Raina Julia Amanda , Welsi Wandila , Diouf Maulana Dwi Putranto , Rismiyati Rismiyati , Anggit ListyacahyaniDOI:
10.29303/jbt.v25i1.8480Published:
2025-02-13Issue:
Vol. 25 No. 1 (2025): Januari - MaretKeywords:
Antibacterial, butterfly pea, flower, kombucha.Articles
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Abstract
Biotechnology involves the use of microorganisms supported by technology to produce goods and services, a practice that has been around for thousands of years. The application of biotechnology includes processing fermented beverage products. Kombucha is a traditional fermented tea drink that is carbonated and has a slightly sweet and sour taste. Butterfly pea flower contains anthocyanins, which act as antioxidants and antibacterial agents. Sugar serves as a nutrient for Scoby, helping with the fermentation process. This article review uses a scientific literature study, with data collected from Google Scholar, referencing 15 journals from the last 10 years. Scoby nutrients can include granulated sugar, palm sugar, Baduy forest honey, stevia sugar, and Tropicana Slim sugar. Each nutrient is given at concentrations of 20%, 30%, and 40%. The test results show that providing nutrients at a concentration of 40% produced a larger inhibition zone.
References
Abdilah, N. A., Rezaldi, F., Kusumiyati, K., Sasmita, H., & Somantri, U. W. (2022). Aktivitas Antibakteri Kombucha Bunga Telang (Clitoria ternatea L.) yang Difermentasi dengan Gula Aren pada Konsentrasi Berbeda. Tirtayasa Medical Journal, 1(2), 29-39. https://doi.org/10.52742/tmj.v1i2.15139
Antolak, H., Piechota, D., & Kucharska, A. (2021). Kombucha Tea—A Double Power of Bioactive Compounds from Tea and Symbiotic Culture of Bacteria and Yeasts (SCOBY). Antioxidants, 10. https://doi.org/10.3390/antiox10101541.
Bhattacharya, D., Ghosh, D.,l Bhattacharya, S., Sarkar, S., Karmakar, P., Koleyb, H., & Gachhui, R. (2018). Comparisonl between differentl D-Dimer cutoff values to assess the individual risk of recurrent venous thromboembolism: Analysis of results obtainedl in the DULCIS study. Letters in Applied Microbiology, 66(2), 145–152. https://doi.org/10.1111/ijlh.12426
Chen, C., Chen, L., Mao, C., Jin, L., Wu, S., Zheng, Y., Cui, Z., Li, Z., Zhang, Y., Zhu, S., Jiang, H., & Liu, X. (2023). Natural Extracts for Antibacterial Applications.. Small, e2306553. https://doi.org/10.1002/smll.202306553.
Deng, H., Meng, X., Xue, B., & Li, L. (2024). Unveiling the antibacterial potential of anthocyanins - a comprehensive review on this natural plant extract.. Critical reviews in food science and nutrition, 1-14 https://doi.org/10.1080/10408398.2024.2411411.
Fathurrohim, M. F., Rezaldi, F., Abdilah, N. A., Fadillah, M. F., & Setyaji, D. Y. (2022). Pengaruh Metode Bioteknologi Fermentasi Kombucha Bunga Telang (Clitoria ternateal L.) Sebagai Antibakteri Propinobacterium acne. Simbiosa, 11(1), 16–25. http://dx.doi.org/10.33373/sim-bio.v11i1.4244
Fathurrohim, M. F., Rezaldi, F., Safitri, E., Setyaji, D. Y., Rahmi Fadhillah, F., Fadillah, M. F., Hidayanto, F., & Kolo, Y. (2022). Analisis Potensi Fermentasi Kombucha Bunga Telang (Clitoria ternatea L.) dengan Konsentrasi Gula Stevia sebagai Inhibitor Pertumbuhan Bakteri Patogen. Jurnal Jeumpa, 9(2), 729–738. https://doi.org/10.33059/jj.v9i2.6357
Kaashyap, M., Cohen, M., & Mantri, N. (2021). Microbial Diversity and Characteristics of Kombucha as Revealed by Metagenomic and Physicochemical Analysis. Nutrients, 13. https://doi.org/10.3390/nu13124446.
Kolo, Y., Rezaldi, F., Fadillah, M. F., Trisnawati, D., Pamungkas, B. T., Ma’ruf, A., & Pertiwi, F. D. (2022). Antikolesterol Pada Ayam Boiler (Gallus domesticus) dari Bunga Telang (Clitoria ternateal L) Melalui Metode Bioteknologi Fermentasi Kombucha. Jurnal Teknologi Pangan Dan Ilmu Pertanian (Jipang), 4(2), 30–36. https://doi.org/10.36526/jipang.v4i2.2682
Kusumiyati, K., Setyaji, D. Y., Fadillah, M. F., & Rezaldi, F. (2022). Uji Daya Hambat Madu Hutan Baduy Sebagai Substrat Padal Bunga Telang (Clitoria ternatea L)l Melalui Metode Bioteknologi Fermentasi Kombucha dalam Menghambat Pertumbuhan Bakteri Patogen. MEDFARM: Jurnal Farmasi Dan Kesehatan, 11(2), 142–160. https://doi.org/10.48191/medfarm.v11i2.109
Ma’rufa, A., Endang Safitri, Retna Yulrosly Ningtias, Fernanda Desmak Pertiwid, & Firman Rezaldi. (2022). Antibakteri Gram Positif dan Negatif dari Sediaan Sabun Cuci Piring Fermentasi Kombucha Bunga Telang (Clitoria ternatea L) Sebagai Produk Bioteknologi Farmasi. Jurnal Kesehatan Dan Kedokteran, 1(2), 16–25. https://doi.org/10.56127/jukeke.v1i2.115
Mizzi, L., Maniscalco, D., Gaspari, S., Chatzitzika, C., Gatt, R., & Valdramidis, V. P. (2020). Assessing the individual microbial inhibitory capacity of different sugars against pathogens commonly found in food systems. Letters in Applied Microbiology, 71(3), 251–258. https://doi.org/10.1111/lam.13306
Mu’jijah, M., Abdilah, N. A., Rezaldi, F., Kusumiyati, K., Setyaji, D. Y., & Fadillah, M. F. (2023). Fermentasi Bunga Telang (Clitoria ternatea L) Dengan Penambahan Madu Baduy Produk SR12 Sebagai Inovasi Bioteknologi Kombucha. Jurnal Ilmiah Biosaintropis (Bioscience-Tropic), 8(2), 1– 17. https://doi.org/10.33474/e-jbst.v8i2.496
Nyiew, K., Kwong, P., & Yow, Y. (2022). An overview of antimicrobial properties of kombucha.. Comprehensive reviews in food science and food safety. https://doi.org/10.1111/1541-4337.12892.
Puspitasari, M., Rezaldi, F., Handayani,l E. E., & Jubaedah, D. (2022). Kemampuan Bunga Telang (Clitorial ternateal L) Senagai Antimokroba (Listeria monocytogenes, Staphylococcus hominis, Trycophyton mentagrophytes, dan Trycophyton rubrum) Melalui Metode Bioteknologi Fermentasi Kombucha. Jurnal Medical Laboratory, 1(2), 1–10. https://doi.org/10.57213/medlab.v1i2.36
Rezaldi, F., Hidayanto, F., Setyaji, D. Y., Fathurrohim, M. F., & Kusumiyati. (2022). Bioteknologi Kombucha Bunga Telang (Clitoria ternatea L) Sebagai Antibakteri Streptococcus mutan dan Klebsiella pneumoniae Berdasarkan Konsentrasi Gula yang Berbeda Beda. Jurnal Farmagazine, IX(2), 21–27. http://dx.doi.org/10.47653/farm.v9i2.608
Rezaldi, F., Junaedi, C., Ningtias, R. Y., Pertiwi, F. D., Sasmita, H., Somantri, U. W., & Fathurrohim, M. F. (2022). Antibakteri Staphylococcus aureus dari Sediaan Sabun Mandi Probiotik Kombucha Bunga Telang (Clitoria Ternatea L) Sebagai Produk Bioteknologi. Jurnal Biotek, 10(1), 36–51. https://doi.org/10.24252/jb.v10i1.2702
Rezaldi, F., Ningtyas,l R. Y., Anggraeni, S. D., Ma’ruf, A., Fatonah, N. S., Pertiwi, F. D., Fitriyani, F., A, L. D., US, S., Fadillah, M. F., & Subekhi, A. I. (2021). Pengaruh Metode Bioteknologi Fermentasi Kombucha Bunga Telang (Clitoria ternatea L) Sebagai Antibakteri Gram Positif dan Negatif. Jurnal Biotek, 9(2),169 https://doi.org/10.24252/jb.v9i2.25467
Rezaldi, F., Rachmat, O., Fadillah, M. F., Setyaji, D. Y., & Saddam, A. (2022). Bioteknologi Kombucha Bunga Telang (Clitorial ternatea L) Sebagai Antibakteri Salmonella thypi dan Vibrio parahaemolyticus Berdasarkan Konsentrasi Gula Aren. Jurnal Gizi Kerja Dan Produktivitas, 3(1), 13–22. https://doi.org/10.52742/jgkp.v3i.14724
Rezaldi, F., Sasmita, H., Somantri, U. W., Kolo, Y., & Meliyawati. (2022). Pengaruh Metode Bioteknologi Fermentasi Kombucha Bunga Telang (Clitorial ternateaa L.) Sebagai Antibakteri Gram Positif-Negatif Berdasarkan Konsentrasi Gula Tropicanaslim Yang Berbeda-Beda. Jurnal Ilmiah Kefarmasian, 4(1), 1–13. https://doi.org/10.36760/jp.v4i.373
Saddam, A., Rezaldi, F., Ma’ruf, A., Pertiwi, F. D., Suyamto, S., Hidayanto, F., & Kusumiyati, K. (2022). Uji Daya Hambat Bakteri Staphylococcus capitis Bacillus cereus dan Pantoea dispersa Melalui Metode Bioteknologi Fermentasi Kombucha Bunga Telang (Clitorial ternatea L). Jurnal Gizi Kerja Dan Produktivitas, 3(2), 65. https://doi.org/10.52742/jgkp.v3i2.1748
Singh, R. (2017). Principles and Applications of Environmental Biotechnology for a Sustainable Future. Principles and Applications of Environmental Biotechnology for a Sustainable Future. https://doi.org/10.1007/978-981-10-1866-4
Somantri, U. W., Fadillah, M. F., Rezaldi, F., Pruschia, D. I., Margarisa, D., & Maharani, M. (2023). In Vitro Pharmacological Activity Test of Telang Flower Kombucha as Antibacterial Vibrio cholerae and Shigella dysenteriae Through Fermentation Biotechnology Method. BIOTIK: Jurnal Ilmiah Biologi Teknologi Dan Kependidikan, 11(2), 130–146. https://doi.org/10.22373/biotik.v11i2.17427
Suhartatik, N., Mustofa, A., & Mursito, P. (2019). Phenolic Content and Antioxidant Activity of Black Glutinous Rice Anthocyanin during Fermentation by Pediococcus pentosaceus N11.16. agriTECH. https://doi.org/10.22146/agritech.36347
Vdovenko, V. (2024). Innovative Biotechnology: New Research In The Industry. Collection Of Scientific Papers. https://doi.org/10.23947/interagro.2024.420-423
Villarreal-Soto, S., Beaufort, S., Bouajila, J., Souchard, J., & Taillandier, P. (2018). Understanding Kombucha Tea Fermentation: A Review.. Journal of food science, 83 3, 580-588 . https://doi.org/10.1111/1750-3841.14068
Zailani, N., & Adnan, A. (2022). Substrates And Metabolic Pathways In Symbiotic Culture Of Bacteria And Yeast (Scoby) Fermentation: A Mini Review. Jurnal Teknologi. https://doi.org/10.11113/jurnalteknologi.v84.18534.
Zhang, W., Wei, L., Xu, R., Lin, G., Xin, H., Lv, Z., Qian, H., & Shi, H. (2020). Evaluation of the Antibacterial Material Production in the Fermentation of Bacillus amyloliquefaciens-9 from Whitespotted Bamboo Shark (Chiloscyllium plagiosum). Marine Drugs, 18. https://doi.org/10.3390/md18020119.
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Copyright (c) 2025 Zerati Hijriadina, Buchary Rahman Ash Shiddiqi, Raina Julia Amanda, Welsi Wandila, Diouf Maulana Dwi Putranto, Rismiyati Rismiyati, Anggit Listyacahyani

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