The Role of Manuka Honey in Protecting Against Cochlear Hair Cell Damage Caused by Diabetes Mellitus in Rats, Assessed Through Otoacoustic Emission
DOI:
10.29303/jbt.v25i3.9599Published:
2025-07-11Downloads
Abstract
Diabetes mellitus is an escalating public health concern worldwide, with Indonesia being one of the countries heavily affected and facing a projected increase in cases. This condition is known to cause a range of complications, including hearing loss, which is frequently observed in diabetic individuals. This study aims to investigate the potential protective effect of honey against cochlear damage induced by diabetes mellitus. This ex-vivo experimental study applied a pretest-posttest control group design. Conducted over five months at the FMIPA USU Animal Laboratory, the study involved 25 healthy adult male Rattus norvegicus Wistar rats (150–250 grams, aged 2–3 months). The rats were divided into three treatment groups and assessed before and after honey administration on days 0, 3, 6, 9, and 12. SNR value differences were analyzed using Repeated Measures ANOVA or the Friedman test. Administration of honey at a dose of 1 g/kg body weight was more effective in preventing the decline of SNR values in diabetic rat models compared to the 2 g/kg dose. Honey demonstrates a protective effect against cochlear hair cell damage in diabetic Rattus norvegicus, as evidenced by SNR values from OAE assessments.
Keywords:
Antioxidant therapy, auditory function, honey, oxidative stress, OAE.References
Agarwal, A. C., Pujary, K., Ganapathy, K., Balakrishnan, R., Nayak, D. R., & Hasan, F. (2013). Pure tone audiometry and otoacoustic emissions for the assessment of hearing loss in diabetic patients. Indian Journal of Otology, 19(1), 13-17. 10.4103/0971-7749.108154.
Aprianty S, Kunaedi A, Falya Y. (2023). The effect of forest honey on blood glucose levels in male white mice (Mus musculus) induced by alloxan. J Pharmacopolium. 6(2):1–7. 10.36465/jop.v6i2.1208.
Babinska, Z., & Ruda-Kucerova, J. (2017). Differential characteristics of ketamine self-administration in the olfactory bulbectomy model of depression in male rats. Experimental and clinical psychopharmacology, 25(2), 84. 10.1037/pha0000106.
Ceylan, S. M., Uysal, E., Altinay, S., Sezgin, E., Bilal, N., Petekkaya, E., ... & Gulbagci, M. E. (2019). Protective and therapeutic effects of milrinone on acoustic trauma in rat cochlea. European Archives of Oto-rhino-laryngology, 276, 1921-1931. 10.1007/s00405-019-05417-5.
Chong, S. J. F., Low, I. C. C., & Pervaiz, S. (2014). Mitochondrial ROS and involvement of Bcl-2 as a mitochondrial ROS regulator. Mitochondrion, 19, 39-48. 10.1016/j.mito.2014.06.002.
El-Shafey, A., Seliem, M., El-Zawahry, S., Shahen, E., & Mahmoud, D. (2015). Effect of honey bee on some antioxidant enzymes and lipid profile in hypercholesterolemic male albino rats. Zagazig University Medical Journal, 21(1), 1-11. 10.21608/zumj.2015.4460.
Erejuwa, O. O., Nwobodo, N. N., Akpan, J. L., Okorie, U. A., Ezeonu, C. T., Ezeokpo, B. C., ... & Sulaiman, S. A. (2016). Nigerian honey ameliorates hyperglycemia and dyslipidemia in alloxan-induced diabetic rats. Nutrients, 8(3), 95. 10.3390/nu8030095.
Eteraf-Oskouei, T., & Najafi, M. (2013). Traditional and modern uses of natural honey in human diseases: a review. Iranian journal of basic medical sciences, 16(6), 731. 10.22038/ijbms.2013.988.
Gioacchini, F. M., Pisani, D., Viola, P., Astorina, A., Scarpa, A., Libonati, F. A., ... & Chiarella, G. (2023). Diabetes mellitus and hearing loss: a complex relationship. Medicina, 59(2), 269. 10.3390/medicina59020269.
Hasan FE, & Yunus R. (2023). Antioxidant function in inhibiting lipid peroxidation and increasing erythrocyte membrane resistance in diabetes mellitus patients. Health Inf J Penelit. 15(2):e901. 10.36990/hijp.v15i2.901.
Lubis, M. F. N., & Haryuna, T. S. H. (2022). The Correlation of Signal to Noise Ratio Value on DPOAE with Malondialdehyde Levels in Rattus Norvegicus Diabetes Model. Iranian Journal of Otorhinolaryngology, 34(123), 185. 10.22038/IJORL.2022.62639.3151
Luka, C. D., Olatunde, A., Tijjani, H., & Olisa-Enewe, I. A. (2013). Effect of aqueous extract of Phaseolus vulgaris L.(red kidney beans) on alloxan-induced diabetic Wistar rats. Int. J. of Science Invention Today, 2(4), 292-301.
Malucelli, D. A., Malucelli, F. J., Fonseca, V. R., Zeigeboim, B., Ribas, A., de Trotta, F., & da Silva, T. P. (2012). Hearing loss prevalence in patients with diabetes mellitus type 1. Brazilian journal of otorhinolaryngology, 78(3), 105-115. 10.1590/S1808-86942012000300018.
Martinotti, S., & Ranzato, E. (2018). Honey, wound repair and regenerative medicine. Journal of functional biomaterials, 9(2), 34. 10.3390/jfb9020034.
Mishra, U. S., Pradhan, S. R., & Palai, S. (2020). Evaluation of left ventricular function in type-2 diabetes mellitus with microalbuminuria. J. Evid. Based Med. Healthc, 7(8), 385-389. 10.18410/jebmh/2020/83.
Paluru, R., Indla, Y. R., Ramasamy, C., & Santhakumari, R. (1966). www. ijrap. net. Adv Otorhinolaryngol, 13. 10.7897/2277-4343.072140.
PERKENI. Consensus on Diabetes Mellitus Management and Prevention in Indonesia. Jakarta: Indonesian Society of Endocrinology 2015;:4–69.
Pieme, C. A., Tatangmo, J. A., Simo, G., Biapa Nya, P. C., Ama Moor, V. J., Moukette Moukette, B., ... & Sobngwi, E. (2017). Relationship between hyperglycemia, antioxidant capacity and some enzymatic and non-enzymatic antioxidants in African patients with type 2 diabetes. BMC Research Notes, 10, 1-7. 10.1186/s13104-017-2463-6.
Priyono, H., Restuti, R. D., Kusuma, I., Harahap, A., Ascobat, P., Setiabudy, R., ... & Hidayati, A. P. N. (2024). In vitro activity of Manuka and Trigona honey on fibroblast and keratinocyte cultures. Oto Rhino Laryngologica Indonesiana, 54(1), 1-10. 10.32637/orli.v54i1.669.
Sachdeva, K., & Azim, S. (2018). Sensorineural hearing loss and type II diabetes mellitus. Int J Otorhinolaryngol Head Neck Surg, 4(2), 499-507. 10.18203/issn.2454-5929.ijohns20180714.
Tanny, S. B., Sumbayak, E. M., Dewajanti, A. M., & Rumiati, F. (2024). The Effectiveness of Pure Honey as Antioxidant and Gastroprotective in Sprague Dawley Rats (Rattus norvegicus). Jurnal MedScientiae, 3(3), 276-286. 10.36452/JMedScientiae.v3i3.3481.
Xipeng, L., Ruiyu, L., Meng, L., Yanzhuo, Z., Kaosan, G., & Liping, W. (2013). Effects of diabetes on hearing and cochlear structures. Journal of Otology, 8(2), 82-87. 10.1016/S1672-2930(13)50017-1
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