An Amperometric Biosensor For Hg(II) Based On Urease/Alginate–Chitosan Membrane Modified Screen Printed Carbon Electrode


Rochmad Kris Sanjaya , Nurul Ismillayli , Dhony Hermanto






Vol. 14 No. 3 (2019): September


Amperometric biosensor, Urease/alginate–chitosan membrane, Screen- printed carbon electrode



How to Cite

Sanjaya, R. K., Ismillayli, N., & Hermanto, D. (2019). An Amperometric Biosensor For Hg(II) Based On Urease/Alginate–Chitosan Membrane Modified Screen Printed Carbon Electrode. Jurnal Pijar Mipa, 14(3), 184–188.


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A screen-printed three-electrode system is fabricated to prepare a novel screen-printed biosensor for rapid determination of Hg(II) in aqueous solution. The amperometric biosensor is prepared by entrapping urease in alginateââ¬âchitosan membrane to modify the screen-printed carbon electrode. The urease/alginateââ¬âchitosan membrane for Hg(II) had optimum measurement conditions at work potential of -0.15 V, pH of 7, urea concentration of 75 mM, response time of 8 seconds, inhibition time of 7 minutes and temperature of 25 ðC. The resulted biosensor characteristic were found to have the range concentration of Hg(II) ion between 40-90 ppb with the detection limit I10% was 66.45 ppb, the coefficient of variance (Cv) was 0.8%, and reactivation was 5 times reuse.


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Author Biographies

Rochmad Kris Sanjaya, Program Studi Farmasi, Fakultas Ilmu Kesehatan, Universitas Kadiri, Kediri–Jawa Timur, Indonesia

Nurul Ismillayli, Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mataram.

Dhony Hermanto, Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mataram.


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