A Simple and Sensitive Optode Sensor Glucose Based on Immobilization Benedict Into Nata Cellulose Membranes


Dhony Hermanto , Rochmad Kris Sanjaya , Nurul Ismillayli






Vol. 15 No. 4 (2020): September


Optical Chemical Sensor, Benedict Reagent, Nata Cellulose, Entrapment, Glucose



How to Cite

Hermanto, D., Sanjaya, R. K., & Ismillayli, N. (2020). A Simple and Sensitive Optode Sensor Glucose Based on Immobilization Benedict Into Nata Cellulose Membranes. Jurnal Pijar Mipa, 15(4), 404–407. https://doi.org/10.29303/jpm.v15i4.1352


Download data is not yet available.


Metrics Loading ...


Determination of glucose concentration in urine and blood that anyone can use at any time is Benedict reagent based chemical sensor. This optical sensor was developed by immobilizing Benedict reagent into nata cellulose as supporting material via entrapment. The nata cellulose/Benedict membrane for glucose determination has optimum condition at maximum wavelenghtà àof 541.57 nm, Benedict concentration of 0.4470 M, and ratio of nata cellulse mass to Benedict volume was 1:3. Characterization of optical sensor for glucose was in working range of 0-5000 ppm, limit of detection was 911,11 ppm, sensitivity was 0.0009 and reproducibility was 0.2295%.


Garris, D. R. & Garris, B. L. (2003). Diabetes-Induced Progressive Endometerial Involution Characterization of Periluminal Epithelial Lipoanathrophy, Experimantal Biology and Medicine, 52(1). 51-58.

Chang, A. S., Dale, A. N. & Moley, K. H. (2005). Maternal Diabetes Adversely Afects Preovulatory Oocyte Maturation, Development and Granulosa Cell Apoptosis, Endocrinology, 146(5). 2445-2453.

Koolman, J. & Rohm, K. H. (2001). Atlas Berwarna dan Teks Biokimia. Terjemahan Dr. Rer. physol dan dr. Septelia Inawati Wanandi. Jakarta: Penerbit Hipokrates.

Koch, Bernd. (2004). The Role of Urine Glucose Testing in the Management of Diabetes Mellitus. Canadian Journal of Diabetes. 28 (1). 238-245

Kuswandi, B., Andreas, R. & Ramaier, N. (2001). Optical Fibre Biosensor Based on Immobilized Enzyme. The Analyst, 126.1469-1491.

Jandura, P., Rield, B. & Kokta, B. V. (2000). Thermal Degraation Behavior of Cellulose Fibers Partially Esterified with Some Long Chain Organic Acids, Polymer Degraation and Stability, 70. 387-394.

Valente, A. J. M., Burrows, H. D., Polishchuk, A. Y., Dominingues, C. P., Borges, O. M. F., Eusebio, M. E. S., Maria, T. M. R., Lobo, V. M. M. & Monkman, A. P. (2005). Permeation of Sodium Dodecyl Sulfate throught Polyaniline modified Cellulose Acetate Membranes, Polymer, 46. 5918-5928.

Tanabe T., Tauma, K., Hamasaki, K. & Ueno, A. (2001). Immobilized fluorescent Cyclodextrin on a Cellulose Membrane as a Chemosensor for Molecule Detection. Journal of Analytical Chemistry, 73. 3126-3130.

Kristinowicz (2005). Molecular Basis of Cellulose Biosynthesis Disappearance in Submerged Culture of Acetobacter xylinum. Jurnal Acta Biochimica Polonica, 52(3). 691-698.

Piluharto, B. 2003. Kajian Sifat Fisik Film Tipis Nata de Coco Sebagai Membran Ultrafiltrasi. Jurnal Ilmu Dasar, 4(1). 52-57.

Rezaee (2005). Role Plasmid in Production of Acetobacter xylinum Biofilms. American Journal of Biochemistry and Biotechnology, 3. 121-125.

Wulandari, R., Hamdiani, S., & Ismillayli, N. (2019). Synthesis Of Mesoporiic Silica From Rice Husk Ash For Pinostrobin Based Drug Delivery. Acta Chimica Asiana, 2(1), 75-82.

Ismillayli, N., Kamali, S. R., Hadi, S., & Hermanto, D. (2019). Synthesis of Biodegradable Superabsorbent Polymers from Carboxymethyl Cellulose/Humic Acid. Acta Chimica Asiana, 2(2), 95-98.

Author Biographies

Dhony Hermanto, Program Studi Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mataram

Rochmad Kris Sanjaya, Program Studi Farmasi, Fakultas Ilmu Kesehatan Universitas Kadiri, Kediri-Jawa Timur

Nurul Ismillayli, Program Studi Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mataram


The following terms apply to authors who publish in this journal:
1. Authors retain copyright and grant the journal first publication rights, with the work simultaneously licensed under a Creative Commons Attribution License 4.0 International License (CC-BY License) that allows others to share the work with an acknowledgment of the work's authorship and first publication in this journal.

2. Authors may enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., posting it to an institutional repository or publishing it in a book), acknowledging its initial publication in this journal.
3. Before and during the submission process, authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website), as this can lead to productive exchanges as well as earlier and greater citation of published work (See The Effect of Open Access).

Similar Articles

1 2 3 4 5 6 7 8 9 > >> 

You may also start an advanced similarity search for this article.