Growth and Development of Mouse Secondary Spermatocytes (Mouse muscle) After Giving Extra Kepel Fruit (Stelechocarpus burahol)
DOI:
10.29303/jpm.v19i6.3415Published:
2024-11-30Issue:
Vol. 19 No. 6 (2024): November 2024Keywords:
Kepel Fruit Extract (Stelechocarpus burahol); Mice (Mouse muscle); Secondary spermatocytes.Articles
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Abstract
Indonesia has a tropical climate is rich in flora diversity. Many medicinal plants, including the Kepel (Stelechocarpus burahol), are herbal medicines. Kepel Fruit (Stelechocarpus burahol) contains secondary metabolites of alkaloids, flavonoids, polyphenols, saponins, triterpenoids and quinones. Some of these compounds have anti-fertility properties. Antifertility compounds are compounds that can prevent fertility by interfering with several normal reproductive mechanisms, both in men and women. This study aims to analyze the effectiveness of Kepel fruit extract on the growth and development of secondary spermatocytes in mice (Mouse muscle). This research uses true experiments and Posttest-Only Control with a quantitative approach. The samples used for this research design, both experimental and controlled, were taken randomly. Testing the significance of the treatment effect uses parametric statistics, namely the One-Way ANOVA test at a significance level of 5% (α = 0.05). Then, if there are differences, continue with the LSD further test (Least Significant Difference) at a significance level of 5% (α = 0.05). This research design used a Randomized Block Design (RAK) with a total sample of 32 mice (Mouse muscle) with 8 repetitions and 3 treatment groups. The growth and development of secondary spermatocytes in mice are determined by counting the number of secondary spermatocytes in the seminiferous tubules of mouse testicular incisions. The results of the one-way Anova test show that the calculated F is 6.41, and the value is sig. (P.Value) of 0.002, these results suggest P.Value (0.002) < the α value (0.05), which means that the number of secondary spermatocytes is significantly different in each group so that this study can conclude that the administration of Kepel fruit extract (Stelechocarpus burahol) can significantly reduce the number of growth and development of mouse secondary spermatocytes (Mouse muscle ).
References
BPS, 2024. Berdasarkan laporan Hasil Proyeksi Penduduk Indonesia 2020-2050 Hasil Sensus Penduduk 2020, 2024,
KB (Keluarga Berencana), 2024.
Departemen Kesehatan Republik Indonesia. Farmakope herbal indonesia edisi I. 2008. Hal: 109-114.
Sunardi, C., Sumiwi, S., A., & Hertati, A. (2010). Penelitian antiimplantasi ekstrak etanol daging buah burahol (Stelechocarpus burahol Hook F. & Thomson) pada tikus putih. Jurnal Majalah Ilmu Kefarmasian. 7(1): 1-8.
Dabhadkar, D., K., Thakare, V., G., Zade, V., S., et al. (2015). Review on some ethnomedicinal plants having antifertility activity in female albino rats. Int. Res. J. of Science & Engineering. 3(2): 41-6.
Batubara, M. S., Sabri, E., & Tanjung, M. (2020). Pengaruh pemberian ekstrak daun andaliman (Zanthoxylum acanthopodium Dc.) terhadap histologis ovarium mencit (Mus musculus L.). BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan). 6(2): 196-209.
Nugroho, R., A. (2018). Mengenal mencit sebagai hewan laboratorium. Samarinda: Mulawarman University Press.
Suparmi, S., Isradji, I., Yusuf, I., Fatmawati, D., dkk. (2015). Antiimplantation activity of kepel (Stelechocarpus burahol) pulp ethanol extract in female mice. The Journal of Pure and Applied Chemistry Research. 4(3): 94-99.
Komala, S., N., Febrianti, P., & Ratini. (2022). Biology notes: fisiologi hewan. Banyumas: Zahira Media Publisher.
Hafez, B., and Hafez, E., S., E. (2000). Reproduction in farm animals 7th ed. Philadelphia: Lea & Febiger.
Nurcholidah, S., Purwantara, B., Supriatna, I., & Winarto, A. (2013). Perkembangan sel-sel spermatogenik dan kualitas sperma pascapemberian ekstrak pegagan (Centella asiatica). JITV. 18 (3):192-201.
Campbell, N., A., Reece, J., B., & Mitchell, L., G. (2004). Biologi, edisi kelima, jilid 3; penerjemah Wasmen Mana. Jakarta: Erlangga.
Arief, Y., S. (2011). Stres dapat mengganggu proses spermatogenesis pada mencit. Jurnal Ners. 6 (2): 169–174.
Anggraito, Y., U., Susanti, R., Iswari, R., S., Yuniastuti, A., (2018). Metabolit sekunder dari tanaman: aplikasi dan produksi. Semarang: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Semarang.
Kusumawati, S., D., Queljoe, E., D., & Fatimawali. (2017). Pengaruh pemberian ekstrak daun lola kahori (Erythrina variegata L) terhadap spermatogenesis tikus putih jantan galur wistar (Rattus norvegicus L). Jurnal Ilmiah Farmasi. 6 (3): 115-119.
Marfu’ah, N., Kasa, I., W., dan, Yowani, S., C. (2014). Pengaruh steroid anabolik methandienone terhadap kuantitas spermatozoa tikus putih (Rattus norvegicus). Jurnal Biologi. 18 (1): 24-27.
Celino FT., Yamaguchi, S., Miura., et al. (2011). Tolerance of spermatogonia to oxidative stress is due to high levels of Zn and Cu/Zn superoxide dismutase. Plos one Journal, Vol 6 (2): e16938.
Trocchia, S., Abdel-Gawad F., K., & Ciarcia G. (2014). Roles of reactive oxygen species in the spermatogenesis regulation. Journal of Frontiers in endocrinology, Vol 5 (56).
Guerriero, G., Trocchia, S., Abdel-Gawad F., K., & Ciarcia G. (2014). Roles of reactive oxygen species in the spermatogenesis regulation. Journal of Frontiers in endocrinology, Vol 5 (56).
Nurkarimah, D., A., Hestianah, E., P., Wahjuni, R., S., dkk. (2017). Effect of Propolis on Spermatogenic Cells Number and Diameter of Seminiferous Tubules in Male Mice (Mus musculus). Journal of KnE Life Sciences, Vol 3 (6): 677-683.
Octavyani, G., K., Kuswanti, N., & Khaleyla, F. (2022). Pengaruh ekstrak daun sawo manila (Manilkara zapota L.) terhadap jumlah sel leydig dan spermatogenik mencit diabetes. Jurnal LenteraBio. 11 (1): 113-121.
Hatmi, R., U., Widyayanti, S., & Sudarmaji. (2015). Potensi kepel (Stelechocarpus burahol [blume] hook. f & th.) sebagai sumber pangan fungsional. Dalam Prosiding Seminar Nasional Sumber Daya Genetik Pertanian.
Nurlely, Aslama, I., A., Cahaya, N., dan Srikartika, V., M. (2022). Efektivitas Ekstrak Etanol Kulit Batang Pakan Banyu (Croton argyratus Blume) terhadap Kualitas dan Kuantitas Spermatozoa sebagai Antifertilitas. Jurnal Pharmascience. 9 (1): 29-39.
Hernawati. (2006). Potensi Buah Pare (Momordica charantia L.) sebagai Herbal Antifertilitas. Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam Universitas Pendidikan Indonesia. Bandung.
Fatmawati, D., Isradji I., Yusuf I., & Suparmi. (2016). Kualitas spermatozoa mencit balb/c jantan setelah pemberian ekstrak buah kepel (Stelechocarpus burahol). Jurnal MKB. 48 (3): 155-159.
Pranadya, N., M., E., Setyawati, I., & Yulihastuti, D., A. (2019). Jumlah sel-sel spermatogenik dan histologis testis mencit (Mus musculus L.) pasca pemberian ekstrak daun kaliandra merah (Calliandra calothyrsus Meissn.) dengan dosis dan interval waktu yang berbeda. Jurnal Biologi Udayana. 23(1): 34-41.
Kaspul. 2007. Kadar testosteron tikus putih Rattus novergicus L setelah mengkonsumsi buah terong tukak (Solanum torvum Sw). Jurnal Bioscientiae. 4(1): 1-8.
Author Biographies
I Wayan Merta, Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Mataram, Indonesia
Kusmiyati Kusmiyati, Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Mataram, Indonesia
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