Determination of flavonoid content of ethanol and ethyl acetate extract from purple passion fruit peel
DOI:
10.29303/jpm.v17i5.3463Published:
2022-09-30Downloads
Abstract
Purple passion fruit is a fruit that is widely cultivated in Indonesia for syrup and juice making. Purple passion fruit has a high nutritional value, such as antioxidants. Passion fruit contains vitamin A, vitamin C, β-carotene, flavonoid, and fiber components. However, passion fruit peel is a waste that can not be appropriately facilitated, so it becomes a waste that can pollute the environment. In addition, the passion fruit peel contains flavonoids that can be used as antidiabetic drugs. This study aimed to determine the difference in flavonoid levels in the peel extract of purple passion fruit by using ethanol and ethyl acetate solvents. The total flavonoid test using the AlCl3 method is then read on a UV-Vis spectrophotometer. The results showed total flavonoid levels of passion fruit peel extract with ethanol and ethyl acetate solvents of 15.01 mg QE/g samples and 1.27 mg QE/g extract. The highest total flavonoid levels were obtained in ethanol extract of 15.01 mg QE/g extract.
Keywords:
Purple Passion Fruit Extract, Total Flavonoids, UV-Vis SpectrophotometerReferences
Septiani, N. (2018). Uji Aktivitas Antibakteri Ekstrak Etanol dan Ekstrak Etanol dan Fraksi-Fraksi Kulit Buah Markisa Ungu (Passiflora edulis Sims) terhadap Bakteri Staphylococcus epidermidis di Laboratorium Fitokimia dan Mikrobiologi Farmasi USU. Universitas Sumatera Utara.
Kusumastuty, I. (2014). Sari Buah Markisa Ungu Mencegah Peningkatan MDA Serum Tikus dengan Diet Aterogenik. Indonesian Journal of Human Nutrition, 1(1), 50–56.
Rozali. (2017). Studi Pemanfaatan Tepung Kulit Buah Markisa (Passifloraedulis Sims) sebagai Bahan Pengawet Minyak Goreng Curah. Univ. Muhammadiyah Sumatera Utara.
Widodo, T. B. N. (2021). Aktivitas Kombinasi Ekstrak Etanol Kulit Markisa (Passiflora edulis Sims) dan Kulit Alpukat (Persea americana Mill) terhadap Kelarutan Kalsium Oksalat. Jurnal Kimia (Journal Of Chemistry), 15(2): 121–130.
Azizah, D. N., Kumolowati, E. & Faramayuda, F. (2014). Penetapan Kadar Flavonoid Metode AlCl3 pada Ekstrak Metanol Kulit Buah Kakao (Theobroma cacao L.). Kartika Jurnal Ilmiah Farmasi, 2(2):45–49.
Sapitri, R. (2020). Analisis Kadar Senyawa Flavonoid Total dan Tanin Total Ekstrak Etanol Kulit Buah Jeruk Manis (Citrus aurantium L.) Menggunakan Metode Spektrofotometri UV-VIS. Universitas Al-Ghifari
Aminah, A., Tomayahu, N. & Abidin, Z. (2017). Penetapan Kadar Flavonoid Total Ekstrak Etanol Kulit Buah Alpukat (Persea americana Mill.) dengan Metode Spektrofotometri UV-Vis. Jurnal Fitofarmaka Indonesia, 4(2): 226–230.
Sa’adah, H., Nurhasnawati, H. & Permatasari, V. (2017). Pengaruh Metode Ekstraksi Terhadap Kadar Flavonoid Ekstrak Etanol Umbi Bawang Dayak (Eleutherine palmifolia (L.) Merr) dengan Metode Spektrofotometri. Borneo Journal of Pharmascientech, 1(1): 1–9.
Sulistyo, S. (2019). Pengaruh Jenis dan Konsentrasi Pelarut terhadap Randemen Ekstrak Flavonoid Daun Sawo Duren (Crysophillum cainito L.) dengan Metode Maserasi. Universitas Muhammadiyah Surakarta.
Pujiastuti, E. & El’Zeba, D. (2021). Perbandingan Kadar Flavonoid Total Ekstrak Etanol 70 % dan 96 % Kulit Buah Naga Merah Hylocereus polyrhizus. Cendekia Journal of Pharmacy, 5(1): 28–43.
Jovanović, A. A., Đorđević, V. B., Zdunić, G. M., Pljevljakušić, D. S., Šavikin, K. P., Gođevac, D. M., Bugarski, B. M. (2017). Optimization of the Extraction Process of Polyphenols from Thymus serpyllum L. Herb Using Maceration, Heat-and Ultrasound-Assisted Techniques. Separation and Purification Technology,179: 369–380.
Chuo, S. C., Nasir, H. M., Mohd-Setapar, S. H., Mohamed, S. F. Ahmad, A., Wani, W. A. Muddassir, M. & Alarifi, A. (2020). Glimpse into the Extraction Methods of Active Compounds from Plants. Critical Reviews in Analytical Chemistry, 1–30,
Ei, D. O. W. (2004). Antioxidant Activity of a Flavonoid-Rich Extract of Hypericum perforatum L. in Vitro. Journal of Agricultural and Food Chemistry, 52: 5032–5039.
Sulaiman, M., Mohammed, S. & Manan, F. A. (2015). Analysis of Total Phenolics, Tannins and Flavonoids From Moringa oleifera Seed Extract. Journal of Chemical and Pharmaceutical Research, 7(1) :132–135.
Azizah, M. N. (2019). Penentuan Aktivitas Antioksidan dan Antidiabetes Ekstrak Daun Ndok-Ndokan (Xanthophyllum Vitellinum). Universitas Jember.
Lestari, E. D. (2021). Penentuan Kadar Flavonoid Total Fraksi N-Heksan, Etil Asetat, dan Air Dari Ekstrak Etanol Bunga Cengkeh (Syzygium aromaticum) dengan Metode Spektrofotometri UV-VIS. Universitas Jenderal Achmad Yani Yogyakarta.
Rebaya, A., Belghith, S. I., Baghdikian, B., Leddet, V. M., Mabrouki, F., Olivier, E., Cherif, J. K., Ayadi, M. T. (2015). Total Phenolic, Total Flavonoid, Tannin Content, and Antioxidant Capacity of Halimium halimifolium (Cistaceae). Journal of Applied Pharmaceutical Science, 5(1): 052–057
Do, Q. D., Angkawijaya, A. E., Tran-Nguyen, P. L. Huynh, L. H., Soetaredjo, F. E., Ismadji, S., Ju, Y. H. (2014). Effect of Extraction Solvent on Total Phenol Content, Total Flavonoid Content, and Antioxidant Activity of Limnophila Aromatica, Journal of Food and Drug Analysis, 22(3): 296–302.
Li, W., Gao, Y., Zhao, J. & Qi, W. (2007). Phenolic, Flavonoid, and Lutein Ester Content and Antioxidant Activity of 11 Cultivars of Chinese Marigold. Journal of Agricultural and Food Chemistry, 55(21): 8478–8484.
Candra, L. M. M., Andayani, Y., & Wirasisya, D. G. (2021). Pengaruh Metode Ekstraksi Terhadap Kandungan Fenolik Total dan Flavonoid Total Pada Ekstrak Etanol Buncis (Phaseolus vulgaris L.). Jurnal Pijar Mipa, 16(3), 397-405.
Calame, T. E. L., Kusmiyati, K., & Merta, I. W. (2021). The Effect of Young Papaya Seed Extract on The Motility and Abnormality of Male Mice Spermatozoes (Mus musculus). Jurnal Pijar Mipa, 16(4), 555-561.
Roanisca, O., Rani, R., & Mahardika, R. G. (2021). Phytochemical Screening and Antibacterial Potency of Jeruk Kunci Fruit Waste (Citrus x microcarpa Bunge) Extract Against Propionibacterium acnes. Jurnal Pijar Mipa, 16(3), 387-392.
Fitriansyah, S. N., Aulifa, D. L., Febriani, Y. & Sapitri, E. (2018). Correlation of Total Phenolic, Flavonoid and Carotenoid Content of Phyllanthus emblica Extract From Bandung With DPPH Scavenging Activities. Pharmacognosy Journal, 10(3): 447–452.
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