Isolation of endophytic bacteria and fungi from soursop (Annona muricata L.) and bioactivity test as antimicrobial against Eschericia coli, Staphylococcus epidermidis, and Candida albicans
DOI:
10.29303/jpm.v18i2.3834Published:
2023-03-30Issue:
Vol. 18 No. 2 (2023): March 2023Keywords:
Endophytic Microbia, Antimicrobial, A. muricata L.Articles
Downloads
How to Cite
Downloads
Metrics
Abstract
This study aimed to test the antimicrobial activity of endophytic bacteria and fungi isolates of soursop (Annona muricata L.) in inhibiting the growth of E.coli, S. epidermidis, and C. albicans. The stages of the research were the isolation of endophytic bacteria, antimicrobial assay, and characterization of potential endophytic bacteria and fungi. Endophytic microbial was isolated from leaves, bark, and root of soursop taken from Praya, Central Lombok. The agar diffusion method was used in the antimicrobial activity assay. Characterization was carried out on endophytic microbes with antimicrobial activity based on colony morphology, Gram staining, and biochemical tests. The antimicrobial activity test results showed that none of the endophytic bacteria and fungi isolates had intense activity inhibiting the test microbes' growth. Isolates with code DN3, DNX, and AKRX had medium activity against E. coli, isolates with code AKR1 had medium activity against S. epidermidis, and isolates with code DN2 AKRX had medium activity against C.albicans. The research results showed that the endophytic bacteria from soursop plants could inhibit the growth of test microbes in the weak to moderate.
References
Pradana, P. Y., Suratmo, S., & Retnowati, R. (2015). Isolasi dan karakterisasi senyawa turunan acetogenin dari daun sirsak (Annona muricata) serta uji toksisitas (Doctoral dissertation, Brawijaya University).
Sriwijayanti, M. B., & Hasan, A. E. Z. AKTIVITAS INHIBISI EKSTRAK ETIL ASETAT BAKTERI ENDOFIT DAUN SIRSAK (Annona muricata L) TERHADAP VIABILITAS KHAMIR Saccharomyces (Inhibition Activity of Ethyl Acetate Extract of Endofit Bacteria from Soursop Leaves (Annona muricata L) against Saccharomyces Yeast. JURNAL ITEKIMA, 10.
Rori, C. A., Kandou, F. E. F., & Tangapo, A. M. (2020). Isolasi dan Uji Antibakteri Dari Bakteri Endofit Tumbuhan Mangrove Avicennia marina. Jurnal Koli, 1(1), 1-7.
Dwidjoseputro, D. (1998). Dasar-dasar mikrobiologi.
Kuntari, Z., Sumpono, S., & Nurhamidah, N. (2017). Aktivitas Antioksidan Metabolit Sekunder Bakteri Endofit Akar Tanaman Moringa oleifera L (Kelor). Alotrop, 1(2).
Castillo, U., Harper, J. K., Strobel, G. A., Sears, J., Alesi, K., Ford, E., ... & Teplow, D. (2003). Kakadumycins, novel antibiotics from Streptomyces sp. NRRL 30566, an endophyte of Grevillea pteridifolia. FEMS Microbiology Letters, 224(2), 183-190.
Simarmata, R., Lekatompessy, S., & Sukiman, H. (2007). Isolasi Mikroba Endofitik dari Tanaman Obat Sambung Nyawa Gynura Procumbens) dan Analisis Potensinya sebagai Antimikroba. Berkala Penelitian Hayati, 13(1), 85-90.
Lestari, N., Rasmi, D. A. C., Zulkifli, L., & Jekti, D. S. D. Isolasi Bakteri Endofit Dari Sea Grass Yang Tumbuh Di Kawasan Pantai Pulau Lombok Dan Potensinya Sebagai Sumber Antimokroba Terhadap Bakteri Patogen. Jurnal Biologi Tropis, 16(2), 75399.
Yuniarti, F., Hidayati, W., & Shofaya, L. (2021). Screening of Antibacterial Potency and Molecular Identification of Endophytic Bacteria from Soursop Leaf (Annona muricata L.).
Otto, M. (2009). Staphylococcus epidermidis—the'accidental'pathogen. Nature reviews microbiology, 7(8), 555-567.
Madigan, M. T., Clark, D. P., Stahl, D., & Martinko, J. M. (2010). Brock biology of microorganisms 13th edition. Benjamin Cummings.
Gunawan, A., Eriawati, E., & Zuraidah, Z. (2018, April). Pengaruh pemberian ekstrak daun sirih (piper sp.) terhadap pertumbuhan jamur candida albicans. In Prosiding Seminar Nasional Biotik (Vol. 3, No. 1).
Manguntungi, B., Al Azhar, R. A. A. M., & Aprilian, K. E. P. T. (2018). Endonesia (Endophyte for Indonesia): Biofertilizer Berbasis Mikroba Endofit guna Meningkatkan Kualitas Pembibitan Budidaya Kelapa Sawit (Elaeis guineensis) di Indonesia. Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati, 44-52.
Suriawiria, U. (2005). Mikrobiologi Dasar. Jakarta: Papas Sinar Sinanti, 172.
Zinniel, D. K., Lambrecht, P., Harris, N. B., Feng, Z., Kuczmarski, D., Higley, P., ... & Vidaver, A. K. (2002). Isolation and characterization of endophytic colonizing bacteria from agronomic crops and prairie plants. Applied and environmental microbiology, 68(5), 2198-2208.
Fibonacci, A., & Hulyadi, H. (2018). Uji Aktivitas Antimikroba Daun Sirsak (Annona muricata L.) Terhadap Bacillus subtillis dan Eschericia coli. Walisongo Journal of Chemistry, 1(1), 14-17.
Silitonga, D. (2019). Uji Aktivitas Antibakteri Ekstrak Etanol dan Etil Asetat Daun Sirsak (Annona muricata L.) terhadap Propionibacterium acnes, Staphylococcus epidermidis dan Pseudomonas aeruginosa (Doctoral dissertation, Universitas Sumatera Utara).
Desriani, D., Safira, U. M., Bintang, M., Rivai, A., & Lisdiyanti, P. (2014). Isolasi dan karakterisasi bakteri endofit dari tanaman binahong dan katepeng china. Jurnal Kesehatan Andalas, 3(2).
Sepriana, C., Jekti, D. S. D., & Zulkifli, L. (2017). Bakteri Endofit Kulit Batang Tanaman Cengkeh (Syzygium aromaticum L.) Dan Kemampuannya Sebagai Antibakteri. Jurnal Penelitian Pendidikan IPA, 3(2).
Backman, P. A., & Sikora, R. A. (2008). Endophytes: an emerging tool for biological control. Biological control, 46(1), 1-3.
Ganiswarna, S. G. (1995). Farmakologi dan Terapi edisi 4.
Rahman, I. A. (2016, September). Aktivitas Antioksidan Metabolit Sekunder Produksi Bakteri Endofit dari Daun Sirsak. In SNSE III 2016.
Zulkifli, L., Jekti, D. S. D., & Bahri, S. (2018). Isolasi, Karakterisasi dan Identifikasi Bakteri Endofit Kulit Batang Srikaya (Annona squamosa) dan Potensinya Sebagai Antibakteri. Jurnal Penelitian Pendidikan IPA, 4(1).
Shore, S. J., & Sathisha, G. (2010). Screening of endophytic colonizing bacteria for cytokinin-like compounds: crude cell-free broth of endophytic colonizing bacteria is unsuitable in cucumber cotyledon bioassay. World Journal of Agricultural Sciences, 6(4), 345-352.
Author Biographies
Nurul Aisyah, Biology Education Study Program, Faculty of Teacher and Training Education, University of Mataram
Lalu Zulkifli, Biology Education Study Program, Faculty of Teacher and Training Education, University of Mataram
Dewa Ayu Citra Rasmi, Biology Education Study Program, Faculty of Teacher and Training Education, University of Mataram
License
Copyright (c) 2023 Nurul Aisyah, Lalu Zulkifli, Dewa Ayu Citra Rasmi
This work is licensed under a Creative Commons Attribution 4.0 International License.
The following terms apply to authors who publish in this journal:
1. Authors retain copyright and grant the journal first publication rights, with the work simultaneously licensed under a Creative Commons Attribution License 4.0 International License (CC-BY License) that allows others to share the work with an acknowledgment of the work's authorship and first publication in this journal.
2. Authors may enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., posting it to an institutional repository or publishing it in a book), acknowledging its initial publication in this journal.
3. Before and during the submission process, authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website), as this can lead to productive exchanges as well as earlier and greater citation of published work (See The Effect of Open Access).
