Effect of Light Intensity on Zebra Fish (Danio rerio) Cell Regeneration

Authors

Adisty Virakawugi Darniwa , Tri Cahyanto , Savira Ngesti Rahayu

DOI:

10.29303/jpm.v19i2.4364

Published:

2024-03-20

Issue:

Vol. 19 No. 2 (2024): March 2024

Keywords:

Caudal Fin; Cell Regeneration; Danio rerio; Light Intensity

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Darniwa, A. V., Cahyanto, T., & Rahayu, S. N. (2024). Effect of Light Intensity on Zebra Fish (Danio rerio) Cell Regeneration. Jurnal Pijar Mipa, 19(2), 260–264. https://doi.org/10.29303/jpm.v19i2.4364

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Abstract

Zebrafish (Danio rerio) is one of the model animals in cell regeneration studies because it has a high ability to regenerate tissue structures, one of which is its caudal fin. This study was conducted to determine the different light interventions on the regeneration of the caudal fin of zebrafish. The research method used is an experimental study with the observed parameters, including specific growth, absolute weight gain, length increase, and survival. A total of 28 male wild-type zebrafish were used in this study at the age of 3 months. The amputated zebrafish on its caudal fin was placed at different light intensities (control, 0 lux, 60 lux, and 90 lux). Parameter measurements were carried out every two days. ANOVA analyzed the data obtained with a significance level of 95%. The results showed that at a light intensity of 90 lux, the zebrafish tail fin regeneration was the most significant based on an increase in total length of 0.10 mm. Using a light intensity of 90 lux is the best condition in the regeneration process of the zebrafish caudal fin. The research findings could be further applied in the healing process of external wounds.

References

Olsen, A. S., Sarras, M. P., & Intine, R. V. (2010). Limb regeneration is impaired in an adult zebrafish model of diabetes mellitus. Wound Repair and Regeneration. 18(85), 532–542.

Mahardhika, K. N., Rejeki, S., & Elfitasari, T. (2017). Performa Pertumbuhan Dan Kelulushidupan Benih Ikan Patin (Pangasius hypophthalamus) Dengan Intensitas Cahaya yang Berbeda. Journal of Aquaculture Management and Technology. 6(4), 130–138.

Ye, Q., Chen, D., Xi, Y., Li, J., Shao, J., & Li, L. (2012). Effects of Light Illumination and The Expression Of Wee1 on Tissue Regeneration in Adult Zebrafish. Biochemical and Biophysical Research Communications, 428(1), 132–136.

Paredes, L. C., Camara, N. O. S., & Braga, T. T. (2019). Understanding The Metabolic Profile of Macrophages During The Regenerative Process in Zebrafish. Frontiers in Physiology. 10(1), 1–19.

Darniwa, A., Cahyanto, T., Eka Putri, Z., Kusumorini, A., Ulfa, R., Adawiyah, A., & Hizqiyah, I. (2021). Uji Perilaku dan Preferensi Area pada Ikan Zebrafish (Danio rerio) yang Diinduksi Stres. Biosfer: Jurnal Biologi Dan Pendidikan Biologi. 5(2), 32-38.

Lisdalia, S. (2013). Pengaruh Intensitas Cahaya Terhadap Kecepatan Regenerasi non-alami Ikan Planaria. Skripsi. Universitas Negeri Semarang.

Uemoto, T., Abe, G., & Tamura, K. (2020). Regrowth Of Zebrafish Caudal Fin Regeneration Is Determined by The Amputated Length. Scientific Reports, 10(1), 1–11.

Reed, B., & Jennings, M. (2011). Guidance On The Housing and Care Of Zebrafish. In Research Animal Department, Science Group, RSPCA. Retrieved from papers2://publication/uuid/4F68076E-5B1F-4412-9366-E2017C6ADCD0

Rahmawati, R., Cindelaras, S., & Kusrini, E. (2016). Keragaman Pertumbuhan dan Warna Ikan WILD BETTA (Betta sp.) Dengan Rekayasa Intensitas Cahaya dan Warna Latar. Jurnal Riset Akuakultur, 11(2), 155–162.

Boykin, J. C. (2016). The Behavioral, Biochemical and Genetic Effects of Sleep Deprivation in Zebrafish (Danio Rerio). Georgia Southern University.

Aras, A. K., Nirmala, K., Soelistyowati, D. T., & Sudarto. (2016). Manipulasi Spektrum Cahaya Terhadap Pertumbuhan dan Kualitas Warna Yuwana Ikan Botia Chromobotia macracanthus. Jurnal Iktiologi Indonesia. 16(1), 45–55.

Schebesta, M., Lien, C. L., Engel, F. B., & Keating, M. T. (2006). Transcriptional profiling of caudal fin regeneration in zebrafish. The Scientific World Journal, 6(S1), 38–54.

Delabbio, J. (2015). Advantages of LED Lighting Systems in Larval Fish Culture. World Aquaculture. 1(1), 25–29.

Sari, Kartika, N., Listyorini, Dwi, Gofur, & Abdul. (2016). Proses Regenerasi Sirip Ekor Pada Ikan Zebra. Edubiotik, 1(1), 25–29.

Putri Anica Rahmawati, A., Hudaidah, S., & Wijayanti, H. (2016). Pengaruh Intensitas Cahaya Selama Pemeliharaan Benih Ikan Kerapu Macan (Epinephelus fuscoguttatus). E-Jurnal Rekayasa Dan Teknologi Budidaya Perairan, 5(1), 547–558.

Sehring, I. M., & Weidinger, G. (2019). Recent Advancements In Understanding Fin Regeneration In Zebrafish. Wiley Interdisciplinary Reviews: Developmental Biology, 9(1), 1–16.

Poss, K. D., Keating, M. T., & Nechiporuk, A. (2003). Tales Of Regeneration In Zebrafish. Developmental Dynamics, 226(2), 202–210.

Matthews, M., Trevarrow, B., & Matthews, J. (2002). A Virtual Tour Of The Guide For Zebrafish Users. Lab Animal. 31(3), 34–40.

Cavalcante, A. K., Rogero, S. O., & Rogero, J. R. (2017). Evaluation Of Resveratrol Toxicity In The Embryolarval Stage Of Danio rerio fish. Ecotoxicol. 12(1), 133–139.

Carrillo, A., & McHenry, M. J. (2016). Zebrafish learn To Forage In The Dark. Journal of Experimental Biology. 219(4), 582–589.

Novianti, T. (2018). Ekspresi Gen Sitoglobin Pada Fase Wound Healing Proses Regenerasi Jaringan. Skripsi. Universitas Essa Unggul.

Fernando, R. (2019). Pengaruh Penambahan Tepung Wortel (Daucus Carota) Pada Pakan Buatan Terhadap Peningkatan Kecerahan Warna Ikan Cupang (Betta splendens Regan). Skripsi. Universitas Muhammadiyah Pontianak.

Adamskaya, N., Dungel, P., Mittermayr, R., Hartinger, J., Feichtinger, G., Wassermann, K., Van Griensven, M. 2011. Light Therapy by Blue LED Improves Wound Healing in an Excision Model in Rats. Injury. 42(9), 917–921.

Gemberling, M., Bailey, T. J., Hyde, D. R., & Poss, K. D. (2013). The Zebrafish As A Model For Complex Tissue Regeneration. Trends in Genetics. 1(1), 1–10.

Bianingrum. (2015). Perbedaan Intensitas Cahaya Terhadap Performa Pertumbuhan dan Sintasan Benih Ikan Sepat Siam Trichopodus pectoralis. Skripsi. Institut Pertanian Bogor.

Fleisch, V. C., & Neuhauss, S. C. (2006). Visual behavior in zebrafish. Zebrafish, 3(2), 191-201.

Author Biographies

Adisty Virakawugi Darniwa, Biology Department, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung

Tri Cahyanto, Biology Department, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung

Savira Ngesti Rahayu, Biology Department, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung

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Copyright (c) 2024 Tri Cahyanto, Adisty Virakawugi, Savira Ngesti Rahayu

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