Effectiveness of snail mucus gel (Achatina fulica Ferr) on mice (Mus musculus L.) burns
DOI:
10.29303/jpm.v18i3.4803Published:
2023-05-28Issue:
Vol. 18 No. 3 (2023): May 2023Keywords:
Achatinafulica, Mus musculus L., Burns HealingArticles
Downloads
How to Cite
Downloads
Metrics
Abstract
This study aimed to determine the effectiveness of snail mucus gel (Achatina fulica) on the length of time for burns healing, burns diameter, and the percentage of burns healing in mice (Mus musculus). The type of this research was experimental with a completely randomized design (CRD) method with 20 male mice divided into five treatment groups, namely NC (Negative Control; gel basis), PC (Positive Control; Bioplacenton 10%), P1 (3% gel), P2 (4% gel) and P3 (5% gel), with four replications for each treatment. The making of burns on the back of mice was done by using metal coins that were heated to become coals with an initial diameter of 20 mm, which are affixed for 1 second. The parameters observed were the length of time for burns healing, burns diameter, and the percentage of burns healing for 16 days. The data were analyzed by using Way ANOVA (Analysis Of Variance) (α = 0.05%) and continued with the LSD (Least Significant Different) test. The results showed that snail mucus gel (A. fulica) with a gel concentration of 5% was more effective in accelerating burns healing with an average healing time of 14 days, and positive controls healed within 14. In 25 days, 4% gel recovered in 14.5 days, 3% in 15 days, and negative controls recovered in 16 days. The fastest closure of burns diameter was in gel with a concentration of 5% with a mean of burns diameter of 0.33 mm and an average percentage of burn healing of 98.35%, followed by the Bioplacenton treatment group of 10%, gel with a concentration of 4%, 3%, and the longest burns closure was in the ointment base gel group.
References
Kumar, B. (2007). Ethnopharmacological Approaches to Wound Healing exploring Medicinal Plants of India, Journal of Ethnopharmacology, 114(2): 103–113
Sulisetyowati, S. D. and Oktariani, M. (2015). Comparison of the Effectiveness of Snail Mucus (Achatina Fulica) with Chitosan on Wound Healing., Journal of Health Kusuma Husada, 6(2)
Suarni, E., Rizki, P. and Badri, A. (2016). Effectiveness Test of Snail Mucus (Achatina fulica compared to Povidone Iodine 10% on Slice Healing (Vulnus scissum) in Mice (Mus musculus), Syifa Medika, 7(1): 9–15
Sumule, A., Kuncahyo, I. and Leviana, F. (2020). Optimization of Carbopol 940 and Glycerin in Snail Slime Gel Formula (Achatina fulica Ferr) as Staphylococcus aureus Antibacterial with Simplex Lattice Design Method, Indonesian Pharmaceutical Journal, 17(1): 108–117.
Widyaningsih, W., Prabowo, A. and Sumiasih. (2010). The Effect of Ethanol Extract from Snail Meat (Achantina Fulica) on Total, Hdl and LDL Cholesterol Levels of Blood Serum of Wistar Male Rats, Journal of Pharmaceutical Science and Technology, 15(1): 1-10.
Moenadjat, Y. (2009). Practical Knowledge of Clinical Burns II Edition. UI Faculty of Medicine, Jakarta.
Wijayantini, R., Cahyaningsih R. and Permatasari A.N. (2018). The effectiveness of 70% Pandan Wangi Leaf Ethanol Extract Ointment on Healing Burns in Male White Mice, Pharmaceutical Scientific Journal, 8(1): 32-42.
Rahim, F., Mimi, A. and Nurwani, P. A. (2011). Creamy Formulation of Sweet Potato (Ipomoea batatas L.) Leaf Ethanol Extract for the Treatment of Burns, Scienta, 1(1): 21–26.
Pramita, F.Y. (2013). Formulation of Antiseptic Gel Preparations of Methanol Extract of Kesum Leaves (Polygonum Minus Huds), Pharmacy Student Journal, Faculty of Medicine, UNTAN, 3(1): 1- 9.
Purnasari, P. W., Fatmawati, D., and Yusuf, I. (2012). The Effect of Snail Mucus (Achatina fulica) on the Number of Fibroblast Cells in Healing Cuts. 4(2): 195–203.
Ghofroh, A.A. (2017). Activity Test of 70% Ethanol Extract of Kitolid Leaves (Isotoma longiflora) on the Acceleration of Healing of Degree II A Burns (Combustion) in Mice (Mus musculus L.), Thesis, UIN Maulana Malik Ibrahim. Poor.
Sumoza, N.S., Efrizal and Rahayu R. (2014). Effect of Giving Gambir (Uncaria gambir R.) on Healing Burns in Male White Mice (Mus musculus L.), Journal of Biology, University of Andalas. 3(4): 1-11.
Handayani, F., Sundu, R. and Karapa, H.N. (2016). Activity Test of Areca catechu L. Ethanol Extract on Healing Burns on the Back Skin of Male White Mice (Mus musculus), Manuntung Scientific Journal. 2(2): 11-18.
Aponno, J. V, Yamlean, P. V. Y. and Supriati, H. S. (2014). Effectiveness Test of Ethanol Extract Gel Preparations of Guava Leaves (Psidium Guajava Linn) on Healing Wounds Infected with Staphylococcus Aureus Bacteria in Rabbits (Orytolagus cuniculus), Pharmacon, 3(3): 279–286.
Wahyudi and Agustina, H. (2018). Ointment of Moringa Leaf Extract (Moringa oleifera Lam.) as Topical Burn Treatment in Rabbits (Oryctolagus cuniculus). Pharmacy Journal. 1(1), 21-24.
Candra, L. M. M., Andayani, Y., & Wirasisya, D. G. (2021). Pengaruh Metode Ekstraksi Terhadap Kandungan Fenolik Total dan Flavonoid Total Pada Ekstrak Etanol Buncis (Phaseolus vulgaris L.). Jurnal Pijar Mipa, 16(3), 397-405.
Harti, A. S., Murharyati, A. and Sulisetyawati, S. D. (2015). Biopreparation of Snail Mucus (Achatina fulica) using Chitosan Membrane as Gauze Dressing for Wound Healing. XII National Seminar on Biology Education FKIP UNS 2015, 923–928.
Mardiyantoro, F., Zefry, Z. A., Astika, S. and Handaru, D. (2020). The Effect of Snail Mucus (Achatina fulica) on the Number of Fibroblasts in the Tooth Socket of Rattus Norvegicus. E-Prodenta Journal of Dentistry, 4(2): 307–313.
Ananta, G. A. P. Y. V. (2020). Potential of Banana Stem (Musa pardisiaca L.) in Healing Burns Introduction, Scientific Journal of Health Sandi Husada, 11(1): 334–340.
Dewi, S.P. (2010). Differences in Effects of Snail Mucus (Achantina fulica) and Bioplacenton Gel on Clean Wound Healing in White Mice, Thesis., Faculty of Medicine, Sebelas Maret University, Surakarta.
Nur, N.B. 2017. Macroscopic Differences in Healing Cuts Between Topical Administration of Human Cord Mesenchymal Stem Cell Extract and Bioplacenton Gel in Male White Rats (Rattus norvegicus) Sprague Dawley Strain, Kripsi, Medical Education Study Program, Faculty of Medicine, University of Lampung, Bandar Lampung.
Cho, H., Ryou, J. and Lee, J. (2008). The effects of the placental extract on fibroblast proliferation. Journal of Cosmet, 20(2): 195–202.
Padua, C.A.M.A., Schnuch, H., Lessmann, J., Geier, A., Pfahlberg, W., Uter,.(2005). Contact allergy to neomycin sulfate results of a multifactorial analysis, Journal of Pharmacoepidemol Drug Saf.,14(10):725–733.
Author Biographies
Wa Ode Harlis, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Halu Oleo
Dwi Arinto Adi, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Halu Oleo
Ni Made Maitri Saraswati, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Halu Oleo
Jamili Jamili, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Halu Oleo
Suriana Suriana, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Halu Oleo
Resman Resman, Department of Soil Science, Faculty of Agriculture, University of Halu Oleo
License
Copyright (c) 2023 Wa Ode Harlis, Dwi Arinto Adi, Ni Made Maitri Saraswati, Jamili Jamili, Suriana Suriana, Resman Resman
This work is licensed under a Creative Commons Attribution 4.0 International License.
The following terms apply to authors who publish in this journal:
1. Authors retain copyright and grant the journal first publication rights, with the work simultaneously licensed under a Creative Commons Attribution License 4.0 International License (CC-BY License) that allows others to share the work with an acknowledgment of the work's authorship and first publication in this journal.
2. Authors may enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., posting it to an institutional repository or publishing it in a book), acknowledging its initial publication in this journal.
3. Before and during the submission process, authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website), as this can lead to productive exchanges as well as earlier and greater citation of published work (See The Effect of Open Access).
