Production of Biogas From a Mixture of Chicken Litter and Cow Manure and Its Effect on the Resulting Volume and C/N Ratio


Hendry Tira , Rudy Sutanto






Vol. 19 No. 1 (2024): January 2024


Biogas; Chicken Litter; Cow Manure; C/N Ratio



How to Cite

Tira, H., & Sutanto, R. (2024). Production of Biogas From a Mixture of Chicken Litter and Cow Manure and Its Effect on the Resulting Volume and C/N Ratio. Jurnal Pijar Mipa, 19(1), 107–112.


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The livestock sector's waste is a potential raw material for producing biogas because it is abundantly available. This experiment aimed to understand the potential of two types of livestock waste to produce biogas. The two sources of raw material were mixed with different variations. The results showed that the volume of fermentation gas produced from livestock waste varied depending on the type and composition of the raw material used. Mixing was done to determine the best variation in producing biogas from chicken litter and cow manure. The volume ratio of litter to cow manure used was (0%:100%), (25%:75%), (50%:50%), (75%:25%), (and 100%:0%), with a total volume of 2 liters. In addition, 10% of EM-4 (effective microorganism) was added to the mixture of livestock waste that had previously been given 2 liters of water. The waste mixture was then fermented for 30 days. The fermentation process was carried out under mesophilic conditions at room temperature and pressure. This study showed that the higher the concentration of chicken litter in the mixture, the higher the carbon-to-nitrogen (C/N) ratio value. On the other hand, if there is a higher composition of cow manure in the mixture, the substrate pH value will be higher. Furthermore, a 50%:50% composition of chicken litter and cow manure produced a high volume of biogas. Similarly, the biogas formation rate in this composition showed the best performance. These results prove that the balance of carbon and nitrogen composition, temperature, and substrate acidity significantly affect biogas production.


Martins, F., Felgueiras, C., Smitkova, M., & Caetano, N. (2019). Analysis of fossil fuel energy consumption and environmental impacts in European countries. Energies, 12, 1–11.

Tabatabaei, M., Aghbashlo, M., Valijanian, E., Kazemi, S. P. H., Nizami, A. S., Ghanavati, H., Sulaiman, A., Mirmohamadsadeghi, S., & Karimi, K. (2020). A comprehensive review on recent biological innovations to improve biogas production, Part 1: Upstream strategies vol 146. Elsevier Ltd.

Xu, Z. X., Song, H., Zhang, S., Tong, S. Q., He, Z. X., Wang, Q., Li, B., & Hu, X. (2019). Co-hydrothermal carbonization of digested sewage sludge and cow dung biogas residue: Investigation of the reaction characteristics. Energy, 187, 115972.

Wang, Z., Ali, S., Akbar, A., & Rasool, F. (2020). Determining the influencing factors of biogas technology adoption intention in Pakistan: The moderating role of social media. International Journal of Environmental Research and Public Health, 17(7), 2311.

Kapoor, R., Ghosh, P., Kumar, M., Sengupta, S., Gupta, A., Kumar, S. S., Vijay, V., Kumar, V., Kumar, V. V., & Pant, D. (2020). Valorization of agricultural waste for biogas based circular economy in India: A research outlook. Bioresource Technology, 304. 123036.

Rattanaphan, S., Rungrotmongkol, T., & Kongsune, P. (2020). Biogas improving by adsorption of CO2 on modified waste tea activated carbon. Renewable Energy, 145, 622–631.

Barber, W. P. F. (2016). Thermal hydrolysis for sewage treatment: A critical review. Water Research, 104. 53–71.

Vijin, P. A., Sivaram, A. R., Prabhu, N., & Sundaramahalingam, A. (2021). A study of enhancing the biogas production in anaerobic digestion. Materials Today Proceedings, 45(9). 7994–7999.

Herrero, G. N., Benedetti, M., & Bolzonella, D. (2019). Effects of Enzymes Addition on Biogas Production From Anaerobic Digestion of Agricultural Biomasses. Waste and Biomass Valorization, 10, 3711–3722.

Bhatnagar, N., Ryan, D., Murphy, R., & Enright, A. M. (2020). Trace element supplementation and enzyme addition to enhance biogas production by anaerobic digestion of chicken litter. Energies, 13(13), 3477.

Tira, H. S., Padang, Y. A., & Sukrenewita, I. K. (2021). Analysis of cross-sectional area of digester on biogas production rate. Dinamika. Teknik Mesin, 11(1), 68-73.

Tira, H. S., & Umbara, E. G. (2018). Evaluasi efektifitas effective microorganism-4 (EM-4) dalam menaikkan volume produksi biogas. Dinamika Teknik Mesin, 8(1), 40–44.

Zeng, W., Wang, D., Wu, Z., He, L., Luo, Z., & Yang, J. (2021). Recovery of nitrogen and phosphorus fertilizer from pig farm biogas slurry and incinerated chicken manure fly ash Science of the Total Environment. 782, 146856.

Parsaee, M., Kiani, D., & Karimi, K. (2019). A review of biogas production from sugarcane vinasse. Biomass and Bioenergy, 122, 117–125.

Xue, S., Wang, Y., Lyu, X., Zhao, N., Song, J., Wang, X., & Yang, G. (2020). Interactive effects of carbohydrate, lipid, protein composition and carbon/nitrogen ratio on biogas production of different food wastes. Bioresource Technology, 312, 123566.

Yan, L., Liu, C., Zhang, Y., Liu, S., & Zhang, Y. (2021). Effects of C/N ratio variation in swine biogas slurry on soil dissolved organic matter: Content and fluorescence characteristics. Ecotoxicology and Environmental Safety, 209, 111804.

Purba, R. A. P., Yuangklang, C., & Paengkoum, P. (2020). Enhanced conjugated linoleic acid and biogas production after ruminal fermentation with piper betle l. Supplementation Ciencia Rural, 50(7), 1–10.

Kamyab, B., & Zilouei, H. (2021). Investigating the efficiency of biogas production using modelling anaerobic digestion of baker's yeast wastewater on two-stage mixed-UASB reactor. Fuel, 285, 119198.

Du, J., Qian, Y., Xi, Y., & Lü, X. (2019). Hydrothermal and alkaline thermal pretreatment at mild temperature in solid state for physicochemical properties and biogas production from anaerobic digestion of rice straw. Renewable Energy, 139, 261–267

Li, K., Wang, K., Wang, J., Yuan, Q., Shi, C., Wu, J., & Zuo, J. (2020). Performance assessment and metagenomic analysis of full-scale innovative two-stage anaerobic digestion biogas plant for food wastes treatment. Journal of Cleaner Production. 264, 121646.

Carrillo-Reyes, J., Buitrón, G., Arcila, J. S., & López-Gómez, M. O. (2021). Thermophilic biogas production from microalgae-bacteria aggregates: biogas yield, community variation and energy balance. Chemosphere, 275, 129898.

Miah, M. R., Rahman, A. K. M. L., Akanda, M. R., Pulak, A. & Rouf, M. A. (2016). Production of biogas from poultry litter mixed with the co-substrate cow dung Journal of Taibah University for Science, 10(4), 497–504.

Author Biographies

Hendry Tira, university of mataram

Rudy Sutanto, university of mataram


Copyright (c) 2024 Hendry Tira, Rudy Sutanto

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