Antibacterial Activity Test of the Combination of Methanol Extract of Arumanis Mango Leaves (Mangifera indica L) and Taro Leaves (Colocasia esculanta L) on Escherichia coli Bacteria Causes of Diarrhea Diseases

Authors

Siska Kumalasari , Laeli Fitriyati , Titi Pudji Rahayu , Naelaz Zukhruf Wakhidatul Kiromah

DOI:

10.29303/jpm.v19i1.5850

Published:

2024-01-23

Issue:

Vol. 19 No. 1 (2024): January 2024

Keywords:

Antibacterial; Arumanis Mango Leaves; Escherichia coli; Taro Leaves

Articles

Downloads

How to Cite

Kumalasari, S., Fitriyati, L., Rahayu, T. P., & Kiromah, N. Z. W. (2024). Antibacterial Activity Test of the Combination of Methanol Extract of Arumanis Mango Leaves (Mangifera indica L) and Taro Leaves (Colocasia esculanta L) on Escherichia coli Bacteria Causes of Diarrhea Diseases. Jurnal Pijar Mipa, 19(1), 131–135. https://doi.org/10.29303/jpm.v19i1.5850

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Abstract

Arumanis mango leaves (Mangifera indica L) and taro leaves (Colocasia esculanta L) are plants that have the potential to be antibacterial and can be used as an alternative to treat infectious diseases caused by bacteria. Arumanis mango leaves contain secondary metabolite compounds, including flavonoids, alkaloids, steroids, polyphenols, tannins, and saponins, and taro leaves contain secondary metabolite compounds in the form of saponins, tannins, flavonoids, formic acid, glucosides, citric acid, and several minerals. Secondary metabolite compounds that have potential as antibacterials are flavonoids and tannins. This study aims to determine the antibacterial activity of a combination of methanol extracts of arumanis mango leaves (Mangifera indica L) and taro leaves (Colocasia esculanta L) against Escherichia coli bacteria. The research was carried out starting from the plant determination and preparation of simplisia, extraction, phytochemical screening of extracts and KLT tests, and antibacterial testing using the disc paper method. The concentration series of extract combinations used are 6.25%, 12.5%, and 25%. The antibacterial test results of the combination of arumanis mango leaf extract (Mangifera indica L) and taro leaf (Colocasia esculanta L), which showed the highest inhibition zone diameter was at a concentration of 6.25% (1:1), a concentration of 12.5% (1:2). At a concentration of 25% (2:1). From the results of this study, a single extract of arumanis mango leaves and taro leaves with a concentration of 6.25%; 12.5%; 25% and a combination of extracts in a ratio of 1:1. 1:2, 2:1, which has potential antibacterial activity against Escherichia coli bacteria, so that further research can be carried out by utilizing a combination of arumanis mango leaf extracts and taro leaves and as a source of information and insight into the combination of arumanis mango leaves and taro leaves that have antibacterial properties.

References

Riskesdas. (2018). Laporan Provinsi Jawa Tengah Riskesdas 2018. Jawa Tengah: Kementerian Kesehatan RI.

Dinas Kesehatan, P. J. (2019). Profil Kesehatan Provinsi Jateng Tahun 2019. Jawa Tengah: Dinas Kesehatan Priovinsi Jawa Tengah

Elliott, T., Worthington, T., Osman, H., & Gill, M. (2013). Mikrobiologi Kedokteran dan Infeksi Edisi 4. Jakarta: Penerbit Buku Kedokteran EGC.

Rini, C. S., & Rohmah, J. (2020). Buku Ajar Mata Kuliah Bakteriologi Dasar. Umsida Press, 1-108.

Mata, U. M. S. S. T., & Keperawatan II, K. I. D. (2020). Penggolongan Obat, Farmakodinamika Dan Farmakokinetik, Indikasi Dan Kontraindikasi Serta Efek Samping Obat.

Surjowardojo, P., Susilawati, TE, & Sirait, GR (2016). Efek Penghambatan Rebusan Kulit Apel (Malus sylvestrs Mill.) terhadap Pertumbuhan Staphylococcus aureus dan Pseudomonas sp. Penyebab Mastitis pada Sapi Perah. TERNAK TROPIS Jurnal Produksi Ternak Tropis , 16 (2), 40-48.

Ningsih, D. R., Zusfahair, M. D., & Mantari, D. (2017). Ekstrak Daun Mangga (Mangifera indica L.) Sebagai Antijamur Terhadap Jamur Candida albicans dan Identifikasi Golongan Senyawanya. Jurnal Kimia Riset, 2(1), 61-68.

Pranata, C., Tarihoran, S. N., & Darmirani, Y. (2021). Uji Aktivitas Antibakteri Ekstrak Daun Talas (Colococasia esculenta L.) Terhadap Bakteri Escherichia coli. JURNAL FARMASIMED (JFM), 4(1), 19-24.

Rahman, I. W., Fadlilah, R. N., Kristiana, H. N., & Dirga, A. (2022). Potensi Ekstrak Daun Jambu Biji (Psidium guajava) dalam Menghambat Pertumbuhan Serratia marcescens. Jurnal Ilmu Alam dan Lingkungan, 13(1).

Sari, S. M. (2017, December). Efektivitas Antibakterial Fraksi Aktiv Daun Mangga (Mangifera Indica L) Terhadap Escherichia coli ATCC 25922 DAN Salmonella typhi. In Proceeding Seminar Nasional Keperawatan (Vol. 3, No. 1, pp. 73-85).

Pulungan, A. S. S., & Brata, W. W. W. (2017). Aktivitas Antibakteri Ekstrak Etanol Daun Talas Terhadap Bakteri Patogen. Jurnal Penelitian Saintika, 17(2), 76-79.

Ningsih, D. R., Purwati, P., Zusfahair, Z., & Nurdin, A. (2019). Hand Sanitizer Ekstrak Metanol Daun Mangga Arumanis (Mangifera indica L.). ALCHEMY Jurnal Penelitian Kimia, 15(1), 10-23.

Imtihani, H. N., Wahyuono, R. A., & Permatasari, S. N. (2020). Biopolimer Kitosan Dan Penggunaannya Dalam Formulasi Obat. Penerbit Graniti.

Julianto, T. S. (2019). Fitokimia Tinjauan Metabolit Sekunder dan Skrining Fitokimia. vol. 53, no. 9, Yogyakarta: Universitas Islam Indonesia.

Torar, G. M. (2017). Uji Aktivitas Antibakteri Ekstrak Etanol Biji Pepaya (Carica papaya L.) Terhadap Bakteri Pseudomonas aeruginosa dan Staphylococcus aureus. Pharmacon, 6(2).

Hanifa, H. N., Kurniasih, N., Rosahdi, T. D., & Rohmatulloh, Y. (2022, March). Uji Antibakteri Ekstrak Etanol Daun Mangga Arumanis (Mangifera indica L.) Terhadap Esherichia coli. In Gunung Djati Conference Series (Vol. 7, pp. 70-76).

Winastri, N. L. A. P., Muliasari, H., & Hidayati, E. (2020). Aktivitas Antibakteri Air Perasan Dan Rebusan Daun Calincing (Oxalis corniculata L.) Terhadap Streptococcus mutans. Berita Biologi, 9(2), 223-230.

Rahmadiah. (2019). Penetapan Beberapa Parameter Spesifik dan Non Spesifik Ekstrak Etanol Daun Asam Jawa (Tamarindus indica L.). Universitas Indonesia, 10-15

Eleazu, C. O. (2016). Characterization of The Natural Products in Cocoyam (Colocasia esculenta) Using GC–MS. Pharmaceutical biology, 54(12), 2880-2885.

Pancu, D. F., Scurtu, A., Macasoi, I. G., Marti, D., Mioc, M., Soica, C., ... & Dehelean, C. (2021). Antibiotics: Conventional Therapy and Natural Compounds with Antibacterial Activity—A Pharmaco-Toxicological Screening. Antibiotics, 10(4), 401.

Hintz, T., Matthews, K. K., & Di, R. (2015). The Use of Plant Antimicrobial Compounds for Food Preservation. BioMed research international, 2015.

Mulangsri, D. A. K., & Zulfa, E. (2020). Uji Aktivitas Antibakteri Ekstrak Terpurifikasi Daun Mangga Arumanis (Mangifera indica l.) dan Identifikasi Flavonoid dengan KLT. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy)(e-Journal), 6(1), 55-62.

Nugraha, A. C., Prasetya, A. T., & Mursiti, S. (2017). Isolasi, Identifikasi, Uji Aktivitas Senyawa Flavonoid Sebagai Antibakteri Dari Daun Mangga. Indonesian Journal of Chemical Science, 6(2), 91-96.

Emelda, E. (2021). Farmakognosi: Untuk Mahasiswa Kompetensi Keahlian Farmasi.

Sholihah, R. H., Fitriyati, L., & Kiromah, N. Z. W. (2023, January). Kombinasi Ekstrak Etanol Daun Jambu Mete (Anacardium occidentale. L) Dan Daun Mangga Arumanis (Mangifera indica. L. var. arumanis) Sebagai Antibakteri Staphylococcus aureus. In Prosiding University Research Colloquium (pp. 1435-1448).

Novita, W. (2016). Uji Aktivitas Antibakteri Fraksi Daun Sirih (Piper betle L) Terhadap Pertumbuhan Bakteri Streptococcus Mutans Secara In Vitro. JAMBI MEDICAL JOURNAL" Jurnal Kedokteran dan Kesehatan", 4(2).

Author Biographies

Siska Kumalasari, Undergraduate Program, Faculty of Health Sciences, University of Muhammadiyah Gombong

Laeli Fitriyati, Universitas Muhammadiyah Gombong

Titi Pudji Rahayu, Undergraduate Program, Faculty of Health Sciences, University of Muhammadiyah Gombong

Naelaz Zukhruf Wakhidatul Kiromah, Undergraduate Program, Faculty of Health Sciences, University of Muhammadiyah Gombong

License

Copyright (c) 2024 Siska Kumalasari, Laeli Fitriyati, Titi Pudji Rahayu, Naelaz Zukhruf Wakhidatul Kiromah

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

The following terms apply to authors who publish in this journal:
1. Authors retain copyright and grant the journal first publication rights, with the work simultaneously licensed under a Creative Commons Attribution License 4.0 International License (CC-BY License) that allows others to share the work with an acknowledgment of the work's authorship and first publication in this journal.

2. Authors may enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., posting it to an institutional repository or publishing it in a book), acknowledging its initial publication in this journal.
3. Before and during the submission process, authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website), as this can lead to productive exchanges as well as earlier and greater citation of published work (See The Effect of Open Access).