The Potential of Burkholderia sp. from Zea mays Roots as Plant Growth Promoting Rhizobacteria
DOI:
10.29303/jpm.v19i3.6554Published:
2024-05-17Issue:
Vol. 19 No. 3 (2024): May 2024Keywords:
Auxin; Burkholderia sp.; Gibberellins; PGPR; Siderophores; Zea mays.Articles
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Abstract
Plant Growth-Promoting Rhizobacteria (PGPR) plays a crucial role in enhancing plant health, while Burkholderia sp. has been identified and has potential as a promising PGPR for maize plants. Recognizing the essential role of PGPR in plant health, this study explores how L-TRP addition might improve PGPR performance for better plant growth. Employing a completely randomized design, the study assesses the effects of Burkholderia sp. as PGPR on maize growth across three treatment groups: PGPR with L-TRP, PGPR without L-TRP, and a control group. The evaluation focuses on plant growth metrics, plant hormone production (auxin and gibberellin), and siderophore activity to gauge plant iron availability. Statistical analysis highlights that L-TRP supplementation notably increases auxin production in the PGPR + L-TRP group, surpassing the PGPR without L-TRP and control groups. Although both PGPR treatments elevate gibberellin levels compared to the control, auxin increase is the most significant outcome, indicating no substantial difference in gibberellin levels between the two PGPR groups. Enhanced siderophore production suggests improved iron assimilation for plants. The findings demonstrate that L-TRP supplementation with PGPR, particularly Burkholderia sp., effectively boosts maize growth, primarily through increased auxin and siderophore production. This combination presents a promising strategy for augmenting agricultural yields, especially for maize productivity, by leveraging the synergistic effects of soil microbes and nutrient supplementation.
References
Sood, G., Kaushal, R., & Gupta, S. (2020). Role of PGPR in Sustainable Agriculture under Changing Scenario of Climate Change. In: Agricultural Impacts of Climate Change. p. 109–18.
Reddy, P.P. (2014). Potential Role of PGPR in Agriculture. In: Plant Growth Promoting Rhizobacteria for Horticultural Crop Protection. p. 17–34.
Sharma K., Sharma, S., & Prasad, S. R. (2019). PGPR: Renewable Tool for Sustainable Agriculture. Int J Curr Microbiol Appl Sci., 8(01), 525–30.
Sivaji, M., Vinoth, R., Tamilkumar, P., Kumar, S., Chandrasekar, A., & Syamala, M. (2016). Isolation and characterization of plant growth promoting Burkholderia spp. Int J Plant Prot., 9(1), 223–9.
Kang, S. M, Khan, A. L, Hussain, J., Ali, L., Kamran M., & Waqas, M., (2012). Rhizonin A from Burkholderia sp. KCTC11096 and its growth promoting role in lettuce seed germination. Molecules, 17(7), 7980–8.
Gao, M., Zhou, J. J., Wang, E. T., Chen, Q., Xu, J., & Sun, J.G. (2015). Multiphasic characterization of a plant growth promoting bacterial strain, Burkholderia sp. 7016 and its effect on tomato growth in the field. J Integr Agric., 14(9), 1855–63.
Nassar, A. H, El-Tarabily, K. A, & Sivasithamparam, K. (2015). Promotion of plant growth by an auxin-producing isolate of the yeast Williopsis saturnus endophytic in maize (Zea mays L.) roots. Biol Fertil Soils., 42(2), 97–108.
Hassen, A. I., Khambani. L.S., Swanevelder, Z. H., Mtsweni, N. P., Bopape. F. L., & van Vuuren, A., (2012). Elucidating key plant growth–promoting (PGPR) traits in Burkholderia sp. Nafp2/4-1b (=SARCC-3049) using gnotobiotic assays and whole-genome-sequence analysis. Lett Appl Microbiol., 73(5), 658–71.
Harahap, R. T., Simarmata, T., Herdiyantoro, D., & Azizah, I. N. R. (2022). Potential use of PGPR based biofertilizer for improving the nutrient availability in soil and agronomic efficiency of upland rice. Kultivasi. 21(3).
Maheshwari, D. K., Dheeman, S., & Agarwal, M. (2015). Phytohormone-Producing PGPR for Sustainable Agriculture. p. 159–82.
Hafsan, Agustina, L., Natsir, A., & Ahmad, A. (2020). The stability of phytase activity from burkholderia sp. Strain HF.7. EurAsian J Biosci., 14(1), 973–6.
Smith, A. (2023). The role of L-tryptophan supplementation on auxin production by Pseudomonas fluorescens. J Plant Growth Regul., 45(2), 210–25.
Shahzad, H., Idrees, A., Hussain, I., Khan, M. A., Sadia, B., & Ullah, S., (2022). Phytohormone biosynthetic potential of different Rhizobium species. Pakistan J Med Heal Sci., 16(1).
Tsukanova, K. A., Сhеbоtаr, V., Meyer, J. J. M., & Bibikova, T. N. (2017). Effect of plant growth-promoting Rhizobacteria on plant hormone homeostasis, South African Journal of Botany, 113(1).
Feldman, L. J. (1980) Auxin biosynthesis and metabolism in isolated roots of maize. Physiol Plant, 49(2), 145–50.
Mokracka, J., Kaznowski, A., Szarata, M., & Kaczmarek, E. (2003). Siderophore-mediated strategies of iron acquisition by extraintestinal isolates of Enterobacter spp. Acta Microbiol Pol., 52(1), 81–6.
Kloepper, J. W., Leong, J., Teintze, M., Schroth, M. N. Enhanced plant growth by siderophores produced by plant growth-promoting rhizobacteria. Nature. 1980;286(5776):885–6.
Murugappan, R., Karthikeyan, M., Aravinth, A., & Alamelu, M. (2012). Siderophore-mediated iron uptake promotes yeast-bacterial symbiosis. Appl Biochem Biotechnol., 168(8), 2170–83.
Sriyosachati, S., & Cox, C. D. (1986). Siderophore-mediated iron acquisition from transferrin by Pseudomonas aeruginosa. Infect Immun., 52(3), 885–91.
Hotta, K., Kim, C. Y., Fox, D. T., & Koppisch, A. T. (2010). Siderophore-mediated iron acquisition in Bacillus anthracis and related strains. Microbiology, 156(1), p. 1918–25.
Ji, C., Juárez-Hernández, R. E., & Miller, M. J. (2012). Exploiting bacterial iron acquisition: Siderophore conjugates.Future Medicinal Chemistry. 4(1), 297– 313.
Venturi, V., Weisbeek, P., & Koster, M. (1995). Gene regulation of siderophore‐mediated iron acquisition in Pseudomonas : not only the For repressor. Mol Microbiol., 17(4), 603–10.
Khalid, S., Asghar, H. N., Akhtar, M. J., Aslam, A, & Zahir, Z. A. (2015). Biofortification of iron in chickpea by plant growth promoting rhizobacteria. Pakistan J Bot. 47(3), 1191–4.
Cohen, A. C., Bottini, R., & Piccoli, P. (2015). Role of Abscisic Acid Producing PGPR in Sustainable Agriculture. p. 259–82.
Karnwal, A. (2019). Production of indole acetic acid by kocuria rosea VB1 and arthrobacter luteolus VB2 under the influence of L-tryptophan and maize root exudates. Biotechnologia.100(1), 29–35.
Chandran, H., Meena, M., & Swapnil, P. (2021). Plant growth-promoting rhizobacteria as a green alternative for sustainable agriculture. Sustainability (Switzerland), 13(1).
Pac ocirc me, A. N., Nad egrave ge A., Farid, B. M., Adolphe, A., & Lamine, B. M. (2016). Plant growth promoting rhizobacteria: Beneficial effects for healthy and sustainable agriculture. African J Biotechnol.,15(27).
Kalitkiewicz, A., & Kȩpczyńsk, E. (2008). The use of rhizobacteria in plant growth promoting process. Biotechnologia, 18(2), 211-219.
Author Biographies
Muh. Khalifah Mustami, Alauddin State Islamic University
Hafsan Hafsan, 1UUniversitas Islam Negeri Alauddin
Asri Asri, Alauddin State Islamic University
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