Microstructure Characterization of Whey Films Using Different Concentrations of Chia Seeds and 35% Sorbitol as Plasticizer


Fahrullah Fahrullah , Djoko Kisworo , Azhary Noersidiq , Bulkaini Bulkaini , Wahid Yulianto , Baiq Rani Dewi Wulandani , Vebera Maslami






Vol. 19 No. 2 (2024): March 2024


Chia Seed; Film; Sorbitol; Microstructure; Whey



How to Cite

Fahrullah, F., Kisworo, D., Noersidiq, A., Bulkaini, B., Yulianto, W., Wulandani, B. R. D., & Maslami, V. (2024). Microstructure Characterization of Whey Films Using Different Concentrations of Chia Seeds and 35% Sorbitol as Plasticizer . Jurnal Pijar Mipa, 19(2), 348–352. https://doi.org/10.29303/jpm.v19i2.6589


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SEM testing was used to evaluate the homogeneity of the film, including pores, cracks, and surface structure, as well as to determine the cross-section and surface structure and the homogeneity of the film solution mixture. The study aims to determine the microstructure characteristics of whey film by adding different concentrations of chia seed and 35% sorbitol as a plasticizer. This study employed three treatments: P1 with 0.5% chia seed, P2 with 0.75% chia seed, and P3 with 1% chia seed. The research method used was descriptive, providing a detailed description of the process. The results indicate that adding more polymer increases the viscosity of the film suspension during the gelatinization process. The film's microstructure was determined by the heating and drying process, as well as the contribution of chia seed gel to the whey film structure. All treatments achieved a Homogeneous film microstructure, but the 1% concentration treatment resulted in a swollen surface structure. The chia seed was evenly mixed into the whey protein matrix, resulting in a uniform structure.


Aleksanyan, K V. (2023). Polysaccharides for biodegradable packaging materials: Past, present, and future (Brief Review). Polymers, 15(2), 451.

Muñoz-Tebar N, Molina A, Carmona M, Berruga M. I. (2021). Use of chia by-products obtained from the extraction of seeds oil for the development of new biodegradable films for the agri-food industry. Foods, 10(3), 620

Nisar, T., Wang, Z. C., Yang, X., Tian, Y., Iqbal, M., & Guo, Y. (2018). Characterization of citrus pectin films integrated with clove bud essential oil: Physical, thermal, barrier, antioxidant and antibacterial properties. International Journal of Biological Macromolecules, 106, 670-680.

Galus, S., & Lenart, A. (2019). Optical, mechanical, and moisture sorption properties of whey protein edible films. Journal of Food Process Engineering, 42(6).

Moghadam, M., Salami, M., Mohammadian, M., Khodadadi, M., & Emam-Djomeh, Z. (2020). Development of antioxidant edible films based on mung bean protein enriched with pomegranate peel. Food Hydrocolloids, 104.

Mouzakitis, C. K., Sereti, V., Matsakidou, A., Kotsiou, K., Biliaderis, C. G., & Lazaridou, A. (2022). Physicochemical properties of zein-based edible films and coatings for extending wheat bread shelf life. Food Hydrocolloids, 132.

Shivangi, S., Dorairaj, D., Negi, P. S., & Shetty, N. P. (2021). Development and characterization of a pectin-based edible film that contains mulberry leaf extract and its bio-active components. Food Hydrocolloids, 121.

Yousuf, B., Sun, Y., & Wu, S. (2022). Lipid and Lipid-containing Composite Edible Coatings and Films. Food Reviews International, 38(1), 574-597.

Herrera‐vázquez, S. E., Dublán‐garcía, O., Arizmendi‐cotero, D., Gómez‐oliván, L. M., Islas‐flores, H., Hernández‐navarro, M. D., & Ramírez‐durán, N. (2022). Optimization of the physical, optical and mechanical properties of composite edible films of gelatin, whey protein and chitosan. Molecules, 27(3), 869.

Mohammadi, M., Mirabzadeh, S., Shahvalizadeh, R., & Hamishehkar, H. (2020). Development of novel active packaging films based on whey protein isolate incorporated with chitosan nanofiber and nano-formulated cinnamon oil. International Journal of Biological Macromolecules, 149, 11-20.

Fahrullah, F., Lilik, R., Kartika, K., Kalisom, U., & Wahid, Y. (2023). Effect of whey protein on thickness, water vapour transmission rate, and water content of gelatin film. Jurnal Pijar MIPA, 18(6), 945–949.

Fahrullah, Ervandi, M., & Rosyidi, D. (2021). Characterization and antimicrobial activity of whey edible film composite enriched with clove essential oil. Tropical Animal Science Journal, 44(3), 369-376.

Fahrullah, F., Ervandi, M., Indrianti, M. A., Suparwata, D. O., Yasin, I. A., Gobel, Y. A., Mokoolang, S., & Mokoginta, M. M. (2021). Mechanical properties of whey composite edible film with the addition of clove essential oil and different types of plasticizer. IOP Conference Series: Earth and Environmental Science, 755(1).

Mishra, D., Khare, P., Singh, D. K., Luqman, S., Ajaya Kumar, P. V., Yadav, A., Das, T., & Saikia, B. K. (2018). Retention of antibacterial and antioxidant properties of lemongrass oil loaded on cellulose nanofibre-poly ethylene glycol composite. Industrial Crops and Products, 114, 68-80.

Otoni, C. G., Avena-Bustillos, R. J., Azeredo, H. M. C., Lorevice, M. V., Moura, M. R., Mattoso, L. H. C., & McHugh, T. H. (2017). Recent advances on edible films based on fruits and vegetables-A Review. Comprehensive Reviews in Food Science and Food Safety, 16(5).

Ribeiro, A. M., Estevinho, B. N., & Rocha, F. (2021). Preparation and incorporation of functional ingredients in edible films and coatings. Food and Bioprocess Technology, 14(2), 209-231.

Janjarasskul, T., & Suppakul, P. (2018). Active and intelligent packaging: The indication of quality and safety. Critical Reviews in Food Science and Nutrition, 58(5), 808-831.

Cinelli, P., Schmid, M., Bugnicourt, E., Wildner, J., Bazzichi, A., Anguillesi, I., & Lazzeri, A. (2014). Whey protein layer applied on biodegradable packaging film to improve barrier properties while maintaining biodegradability. Polymer Degradation and Stability, 108, 151-157.

Schmid, M. (2013). Properties of cast films made from different ratios of whey protein isolate, hydrolyzed whey protein isolate and glycerol. Materials, 6(8), 3254-3269.

Fahrullah, F., Noersidiq, A., & Maruddin, F. (2022). Effects of glycerol plasticizer on physical characteristic of whey-konjac films enriched with clove essential oil. Journal of Food Quality and Hazards Control, 9(4), 226–233.

Fahrullah, F., Bulkaini, B., Kisworo, D., Yulianto, W., Wulandani, B. R. D., & Haryanto, H. (2023). The water content , solubility , and optical properties of whey-gelatin multilayer films enriched with green tea powder. Jurnal Biologi Tropis, 23(4), 491–499.

Fahrullah, F., Radiati, L. E., Purwadi, P., & Rosyidi, D. (2020). The effect of different plasticizers on the characteristics of whey composite edible film. Jurnal Ilmu Dan Teknologi Hasil Ternak, 15(1), 31-37.

Fahrullah, F., Eka Radiati, L., Purwadi, & Rosyidi, D. (2020). The physical characteristics of whey based edible film added with konjac. Current Research in Nutrition and Food Science, 8(1), 333-339.

Azevedo, V. M., Silva, E. K., Gonçalves Pereira, C. F., da Costa, J. M. G., & Borges, S. V. (2015). Whey protein isolate biodegradable films: Influence of the citric acid and montmorillonite clay nanoparticles on the physical properties. Food Hydrocolloids, 43, 252-258.

Teixeira, B., Marques, A., Pires, C., Ramos, C., Batista, I., Saraiva, J. A., & Nunes, M. L. (2014). Characterization of fish protein films incorporated with essential oils of clove, garlic and origanum: Physical, antioxidant and antibacterial properties. LWT, 59(1), 533-539.

Soukoulis, C., Gaiani, C., & Hoffmann, L. (2018). Plant seed mucilage as emerging biopolymer in food industry applications. Current Opinion in Food Science, 22, 28-42.

Campos, B. E., Dias Ruivo, T., da Silva Scapim, M. R., Madrona, G. S., & de C. Bergamasco, R. (2016). Optimization of the mucilage extraction process from chia seeds and application in ice cream as a stabilizer and emulsifier. LWT, 65, 874-883.

Chaves, M. A., Piati, J., Malacarne, L. T., Gall, R. E., Colla, E., Bittencourt, P. R. S., de Souza, A. H. P., Gomes, S. T. M., & Matsushita, M. (2018). Extraction and application of chia mucilage (Salvia hispanica L.) and locust bean gum (Ceratonia siliqua L.) in goat milk frozen dessert. Journal of Food Science and Technology, 55(10), 4148-4158.

Zettel, V., & Hitzmann, B. (2018). Applications of chia (Salvia hispanica L.) in food products. Trends in Food Science and Technology, 80, 43-50.

Chollakup, R., Pongburoos, S., Boonsong, W., Khanoonkon, N., Kongsin, K., Sothornvit, R., Sukyai, P., Sukatta, U., & Harnkarnsujarit, N. (2020). Antioxidant and antibacterial activities of cassava starch and whey protein blend films containing rambutan peel extract and cinnamon oil for active packaging. LWT, 130.

Yousuf, B., Qadri, O. S., & Srivastava, A. K. (2018). Recent developments in shelf-life extension of fresh-cut fruits and vegetables by application of different edible coatings: A review. LWT, 89.

Khazaei, N., Esmaiili, M., Djomeh, Z. E., Ghasemlou, M., & Jouki, M. (2014). Characterization of new biodegradable edible film made from basil seed (Ocimum basilicum L.) gum. Carbohydrate Polymers, 102(1), 199-206.

Maruddin, F., Ratmawati, R., Fahrullah, F., & Taufik, M. (2018). Karakterisitik edible film berbahan whey dangke dengan penambahan karagenan. Jurnal Veteriner, 19(2), 291-297.

Fahrullah, F., & Ervandi, M. (2021). Mikrostruktur edible film whey dangke dengan penambahan karagenan dan plasticizer sorbitol 35%. Jurnal Ilmu Dan Teknologi Peternakan Tropis, 6(2), 26–31.

Fahrullah, F., & Ervandi, M. (2022). Karakterisasi mikrostruktur film whey dengan penambahan konjac glucomannan. Agrointek : Jurnal Teknologi Industri Pertanian, 16(3), 403–411.

Cofelice, M., Cuomo, F., & Chiralt, A. (2019). Alginate films encapsulating lemongrass essential oil as affected by spray calcium application. Colloids and Interfaces, 3(3).

Butt, M. S., Akhtar, M., Maan, A. A., & Asghar, M. (2023). Fabrication and characterization of carnauba wax-based films incorporated with sodium alginate/whey protein. Journal of Food Measurement and Characterization, 17(1), 694-705.

Wu, C., Li, Y., Du, Y., Wang, L., Tong, C., Hu, Y., Pang, J., & Yan, Z. (2019). Preparation and characterization of konjac glucomannan-based bionanocomposite film for active food packaging. Food Hydrocolloids, 89, 682-690.

Fahrullah, F., Kisworo, D., & Noersidiq, A. (2023). Edible film based on whey-chia seed: physical characterization with addition of different plasticizers. Jurnal Penelitian Pendidikan IPA, 9(10), 8554–8562.

Saberi, B., Chockchaisawasdee, S., Golding, J. B., Scarlett, C. J., & Stathopoulos, C. E. (2017). Physical and mechanical properties of a new edible film made of pea starch and guar gum as affected by glycols, sugars and polyols. International Journal of Biological Macromolecules, 104, 345-359.

Telussa, I., Tanasale, M. F. J. D. P., Ridho, A., Madja, A., & Telussa, I. (2023). Synthesis and characterization of biodegradable plastic from tropical marine microalgae Navicula sp . TAD01. Jurnal Penelitian Pendidikan IPA, 9(9), 6877–6884.

Author Biographies

Fahrullah Fahrullah, Universitas Mataram

Djoko Kisworo, Universitas Mataram

Azhary Noersidiq, Universitas Mataram

Bulkaini Bulkaini, Universitas Mataram

Wahid Yulianto, Universitas Mataram

Baiq Rani Dewi Wulandani, Universitas Mataram

Vebera Maslami, Universitas Mataram


Copyright (c) 2024 Fahrullah Fahrullah, Djoko Kisworo, Azhary Noersidiq, Bulkaini Bulkaini, Wahid Yulianto, Baiq Rani Dewi Wulandani, Vebera Maslami

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