Analysis of Bacterial Characteristics Using the Electrical Impedance Spectroscopy Method
DOI:
10.29303/jpm.v19i5.7061Published:
2024-09-29Issue:
Vol. 19 No. 5 (2024): September 2024Keywords:
Escherichia coli; Frequency; Impedance Spectroscopy; Salmonella typhi; Staphylococcus aureusArticles
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Abstract
Microorganisms have various shapes, structures, and characteristics. This study uses the method of electrical impedance spectroscopy aimed at identifying and comparing the characteristics of Escherichia Coli, Salmonella Typhi, and Staphylococcus Aureus. Measurements from 1 Hz to 100,000 Hz show that Salmonella Typhi has the highest impedance value at low frequencies. In contrast, Escherichia Coli impedance decreases consistently, and Staphylococcus Aureus decreases sharply after 10 Hz. Significant changes are observed in the mid-frequency range of 100 Hz to 1000 Hz, with Salmonella Typhi showing the highest impedance values at 100 Hz compared to Staphylococcus Aureus and Escherichia Coli. At 100 Hz, Salmonella Typhi has the highest impedance value with a mass of 0,06 grams at approximately 39.000 Ohms, 0,08 grams at 35.000 Ohm, and 10 grams at 34.000 Ohm. This is followed by Staphylococcus Aureus, with a mass 0f 0,06 grams having an impedance value of 23.000 Ohms, 0,08 grams having a high impedance value of 31.000 Ohm, and 0,10 grams having an impedance value of 15.000 Ohm. Escherichia Coli, with a mass of 0.06 grams, has an impedance value of 9.000 Ohms, 0,08 grams with an impedance value of 5.000 Ohms, and 0,10 grams has an impedance value of 5.000 Ohms. Electrical Impedance Spectroscopy is effective for identifying and comparing Escherichia coli, Staphylococcus aureu, and Salmonella typhi as the intrinsic characteristics of bacterial cells more influence impedance than bacterial mass.
References
Kurniati, I., & Kalsum, U. (2018). Analisis Pertumbuhan Escherichia Coli Dan Salmonella Typhi Pada Media Macconcey Agar (Mca) Dengan Penambahan Ekstrak Daun Jati (Tectona Grandis Lf). Jurnal Analisis Biologi, 2(01).
Febriza, M. A., & Adrian, Q. J. (2021). Penerapan Ar Dalam Media Pembelajaran Klasifikasi Bakteri. Jurnal Bioeduin, 11(1), 10-18.
Holderman, M. V., De Queljoe, E., & Rondonuwu, S. B. (2017). Identifikasi Bakteri Pada Pegangan Eskalator Di Salah Satu Pusat Perbelanjaan Di Kota Manado. Jurnal Ilmiah Sains, 17(1), 13-18.
Cita, Y. P. (2011). Bakteri Salmonella Typhi Dan Demam Tifoid. Jurnal Kesehatan Masyarakat Andalas, 6(1), 42-46.
Levani, Y., & Prastya, A. D. (2020). Demam Tifoid: Manifestasi Klinis, Pilihan Terapi Dan Pandangan Dalam Islam. Al-Iqra Medical Journal: Jurnal Berkala Ilmiah Kedokteran, 3(1), 10-16.
Ruiz-Pérez, N. J., Sánchez-Navarrete, J., & Toscano-Garibay, J. D. (2021). Natural Products For Salmonellosis: Last Decade Research. In Salmonella Spp.-A Global Challenge. Intechopen.
Purnamasari, N., & Fuadi, N. (2022). Uji Kandungan Bakteri Total Coliform Dan Escherichia Coli Air Tanah Di Kabupaten Pangkep. Sainfis: Jurnal Sains Fisika, 2(1), 1-7.
R. Falamy, E. Warganegara, Dan E. Apriliana. (2012). “Detection Of Coliform Bacteria At Cincau Hitam Market Snacks In Traditional And Supermarkets In Bandar Lampung City,” Major. J. Lampung Univ., Vol. Issn 2337-, Hal. 1–9,.
M. Caroff Dan A. Novikov. (2020) “Lipopolysaccharides: Structure, Function And Bacterial Identification 0–9.
N. Khairunnisa, K. Lisa Yuniati, A. St Fahirah Arsal, Dan R. Faisal Syamsu. (2023). “Efektifitas Ekstrak Daun Kemangi & Ekstrak Daun Sirih Merah Sebagai Anti Mikroba Staphylococcus Aureus Penyebab Furunkle,” Fakumi Med. J. J. Mhs. Kedokt., Vol. 3, No. 2, Hal. 106–111.
Febyayuningrum, I. C., Rosyidah, R. A., & Aini, R. (2021). Kontaminasi Bakteri Alat Stetoskop Dengan Media Bap Dan Mca Di Ruang Penyadapan Darah Udd Pmi Kabupaten Sleman Diy. Jurnal Ilmu Kedokteran Dan Kesehatan Indonesia, 1(3), 106-116.
Sugianto, W. (2024). “Identifikasi Tingkat Kematangan Buah Pisang Berdasarkan Karakteristik Impedansi Listrik Dengan Metode Spektroskopi Impedansi Listrik Menggunakan Elektroda 4 Jarum,” Berk. Fis. Indones. J. Ilm. Fis. Pembelajaran Dan Apl., Vol. 15, No. 1, Hal. 24.
Zhang, F., Jin, T., Hu, Q., & He, P. (2018). Distinguishing Skin Cancer Cells And Normal Cells Using Electrical Impedance Spectroscopy. Journal Of Electroanalytical Chemistry, 823, 531-536.
Abasi, S., Aggas, J. R., Garayar-Leyva, G. G., Walther, B. K., & Guiseppi-Elie, A. (2022). Bioelectrical Impedance Spectroscopy For Monitoring Mammalian Cells And Tissues Under Different Frequency Domains: A Review. Acs Measurement Science Au, 2(6), 495-516.
Nabila, F., Widodo, C. S., & Santoso, D. R. (2023). Correlation Of Whole Blood Cell Morphology On Electrical Impedance Spectroscopic Characteristics On The Severity Level Of Ischemic Stroke Patients. International Journal Of Biological, Physical And Chemical Studies, 5(2), 40-49.
Apipah, E. R. (2014). Sintesis Dan Karakteristik Membran Nilon Yang Berasal Dari Limbah Benang. Jurnal Biofisika, 10(1).
Putri, R. R. A., Sulistya, C., & Santoso, D. R. (2017). Analisis Nilai Impedansi Listrik Pada Daging Ikan Nila Yang Disimpan Dalam Lemari Es. Indonesian Journal Of Applied Physics, 6(02), 117-124.
Maulandari, E. Z. (2018). Analisis Hasil Pengukuran Impedansi Listrik Dengan Menggunakan Metode Injeksi Arus Tipe Floating Dan Howland. Malang: Skripsi Ub.
Bobu, F. R. (2023). Analisis Karakteristik Nilai Impedansi Listrik Pada Daging Ikan Nila (Oreochhromis Nilaticus). Optika: Jurnal Pendidikan Fisika, 7(1), 80-86.
Sugianto, W. (2023). Kajian Awal Karakteristik Impedansi Listrik Larutan Nacl Pada Berbagai Konsentrasi Menggunakan Metode Spektroskopi Impedansi Listrik. Magnetic: Research Journal Of Physics And It’s Application Volume 3, No. 1 Maret 2023 E-Issn: 2775-8583.
Tirono, M., Abtokhi, A., & Samsu, F. (2018). Optimasi Medan Listrik Berpulsa Untuk Menurunkan Jumlah Bakteri Staphylococcus Aereus, Salmonella Sp., Dan Escherichia Coli Pada Susu.
Silhavy, T. J., Kahne, D., & Walker, S. (2010). The Bacterial Cell Envelope. Cold Spring Harbor Perspectives In Biology, 2(5), A000414.
Nikolic, P., & Mudgil, P. (2023). The Cell Wall, Cell Membrane And Virulence Factors Of Staphylococcus Aureus And Their Role In Antibiotic Resistance. Microorganisms, 11(2), 259.
Author Biographies
Evangelista Militchia Christy Dasmasela, Biomedical Engineering, PGRI Yogyakarta University
Wahyu Sugianto, Biomedical Engineering, PGRI Yogyakarta University
Amalia Cemara Nur’aidha, Biomedical Engineering, PGRI Yogyakarta University
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Copyright (c) 2024 Evangelista Militchia Christy Dasmasela, Wahyu Sugianto, Amalia Cemara Nur’aidha
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