Cadmium (Cd) Resistance of Isolate Bacteria from Poboya Gold Mining in Palu, Central Sulawesi

Fahruddin Fahruddin, Syahruddin Kasim, Eka Ummi Rahayu

Abstract

The mining industry in Indonesia is growing rapidly, giving an environmental impact from the waste produced because it contains dangerous heavy metals, one of which is cadmium metal (Cd). The purpose to know the ability of cadmium resistance in bacterial isolates. Bacterial isolates were obtained from soil samples from the Poboya Gold Mine field, Palu. Bacterial isolation and selection were carried out by the plate count method, resistance testing using nutrient broth media containing heavy metals Cd, analysis of Cd concentrations using atomic absorption spectrophotometers. From the results of the study, four types of Cd resistant isolates were obtained based on macroscopic and microscopic characterization results, namely isolates EK1, EK2, EK3 and EK4. The two best isolates of Cd resistance, EK2 and EK4 isolates were tested on media containing 20 ppm Cd, showing that both of these isolates were able to significantly reduce Cd concentrations in the supernatant and pellets.

Keywords

resistance; bacterial colonies; cadmium; resistant bacteria

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References

Ahmad, R.Z. (2018). Mikoremediasi Menghilangkan Polusi Logam Beratpada Lahan Bekas Tambang untuk Lahan Peternakan. Wartazoa, 28 (1):41-50. doi:http://dx.doi.org/10.14334/wartazoa.v28i1.1785

Astiti, G. S. & Sugianti, T. (2014). Dampak Penambangan Emas Tradisional Pada Lingkungan dan Pakan Ternak di Pulau Lombok. Sains Peternakan, 12(2): 101-106. https://jurnal.uns.ac.id/Sains Peternakan/article/view/4786/4128

Barokah, G.R., Dwiyitno & Indrianto, N. (2019). Kontaminasi Logam Berat (HG, PB, dan CD) dan Batas Aman Konsumsi Kerang Hijau (Perna virdis) dari Perairan Teluk Jakarta di Musim Penghujan. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan, 14 (12): 95 95-104. doi: 10.15578/jpbkp.v14i2.611

Cyio, M.B., Uswah, H., Isrun, N. & Yuka, S. (2012). Gold Mining Activities and Its Impacts on Land Degradation in Central Sulawesi Indonesia. Journal of Ecotechnology Research, 16 (4):79- 83.https://www.researchgate.net/publication/323881065_Gold_Mining_Activities_and_Its_Impacts_on_Land_Degradation_in_Central_Sulawesi_Indonesia.

Cyio, M.B., Mahfudz1., Takanobu, I., Alam, A., Tomori, K., Nurdin, R., Golar, Muhammad, R., Muhammad, N. A., Muhammad, R. R. & Bohari. (2017). Impact of the Traditional Gold Mine Management on Public Health and Agricultural Land: A Study of Traditional Gold Mining in Poboya, Sausu and Dongi-Dongi Village, Central Sulawesi, Indonesia. Journal of Food, Agriculture & Environment, 15 (4):115-122. https://www.researchgate.net/publication/321314364_Impact_of_the_traditional_gold_mine_management_on_public_health_and_agricultural_land_A_Study_of_traditional_gold_mining_in_Poboya_Sausu_and_Dongi-Dongi_Village_Central_Sulawesi_Indonesia.

Dwiyana, S. & Fahruddin. (2012). Uji Resistensi Antibiotik pada Bakteri Resisten Merkuri (Hg) Yang di Isolasi dari Kawasan Pantai Losari Makassar, Sainsmat, 1 (2): 199-204. https://ojs.unm.ac.id/sainsmat/article/view/744.

Elliott, P., Ragusa, S. & Catcheside, D. (1998). Growth of Sulfate-Reducing Bacteria Under Acidic Conditions In an Upflow Anaerobic Bioreactor As a Treatment System for Acid Mine Drainage. Water Research, 32(12):3724-3370. https://www.sciencedirect.com/science/article/abs/pii/S0043135498001444

Fahruddin & Asadi, A. (2018). Analisis Populasi Bakteri pada Air Asam Tambang Dengan Perlakuan Sedimen Mangrove. Jurnal Ilmu Alam dan Lingkungan, 9 (1): 70-77. http://journal.unhas.ac.id/index.php/jai2/article/view/4875.

Fahruddin, F. Nursiah L. & Asadi, A. (2015). Use of Organic Materials Wetland to Improving the Capacity Sulfate Reduction Bacteria (SRB) of Reduce Sulfate In Acid Mine Water (AMW). Asian Journal of Microbiology Biotechnology & Environmental Sciences, 17 (2):321-324. http://www.envirobiotechjournals.com/article_abstract.php?aid=5967&iid=191&jid=1

Fahruddin, F., As’adi, A. & Nursiah, L. (2018). Treatment of Acid Mine Drainage Waste Using Sediment As Source of Sulfate-Reducing Bacteria to Reduce Sulfates. Pollution Research, 37 (4): 903-907. http://www.envirobiotechjournals.com/article_abstract.php?aid=9085&iid=263&jid=4

Fahruddin, F., Eva, J. & Zaraswati, D. (2019). Antifouling Potential of Thalassia Hemprichii Extract Against Growth of Biofilm-Forming Bacteria. ScienceAsia, (45): 21–27. http://www.scienceasia.org/2019.45.n1/scias45_21.pdf

Fahruddin (2010). Bioteknologi Lingkungan. Penerbit Alfabeta, Bandung. ISBN: 978-602-8800-14-3

Fahruddin, F., Nur, H., As’adi, A., Abdul, W. & Rifaat. (2010). Deteksi Unsur Logam Dengan XRF dan Analisis Mikroba pada Limbah Air Asam Tambang dari Pertambangan di Lamuru - Kabupaten Bone. Jurnal Geocelebes, 4 (1):7-13. http://journal.unhas.ac.id/index.php/geocelebes/article/view/7831

Gaikwad, R. W., Sapkal, V. S. & Sapkal, R. S. (2011). Acid Mine Drainage: A Water Pollution Issue In Mining Industry. International Journal of Advanced Engineering Technology, 2 (4):257-262.

Golar, Muhammad, B., Muhammad, R., Rahmat, B., Bohari, Muhammad, F. P., Muhammad A. & Laihi, A. (2019). Gold Mining and its Impact on Agricultural Land, Public Health, Violation of the Law: A Study on Poboya Traditional Mining, Palu, Indonesia. Indian Journal of Public Health Research and Development, 10 (10):924. https://www.researchgate.net/publication/338137138_Gold_Mining_and_its_Impact_on_Agricultural_Land_Public_Health_Violation_of_the_Law_A_Study_on_Poboya_Traditional_Mining_Palu_Indonesia.

Hasyimuddin, H., Fatmawati, N. & Indriani, I. (2018). Isolasi Bakteri Pengakumulasi Logam Berat Timbal (Pb) Pada Saluran Pembuangan Limbah Industri Kabupaten Gowa. Biotropic: The Journal of Tropical Biology, 2 (2):126-132. DOI: 10.29080/biotropic.2018.2.2.126-132

Ikerismawati, S. (2019). Bioremediasi Pb Oleh Bakteri Indigen Limbah Cair Agar. Jurnal Biosilampari :Jurnal Biologi, (1) 2: 51-58. DOI: https://doi.org/10.31540/biosilampari.v1i2.288

Jamilah & Amri. (2019) Analisis Bakteri Pengakumulasi Logam Berat Timbal (Pb) di Tanah Pembuangan Limbah Industri Non-Pangan. Celebes Biodiversitas, 2 (2):7-13. http://ojs.stkippi.ac.id/index.php/CB/article/view/172/147

Jin, Y., Yaning, L., Yangcui, N. & Lingyan, W. (2018). Review Effects and Mechanisms of Microbial Remediation of Heavy Metals in Soil: A Critical Review. Applied Sciences, 8 (1336): 1- 17. doi: 10.3390/app8081336

Kapahi, M. & Sarita, S. (2019). Bioremediation Options for Heavy Metal Pollution. Journal Health Pollution. 9 (24): 191203. doi: 10.5696/2156-9614-9.24.191203

Mu'minah, Baharuddin, B., Subair, H. & Fahruddin. (2015). Isolation and Screening Bacterial Exopolysaccharide (EPS) From Potato Rhizosphere In Highland And The Potential As A Producer Indole Acetic Acid (IAA). Procedia Food Science (3): 74-81. https://www.researchgate.net/publication/282832227_Isolation_and_Screening_Bacterial_Exopolysaccharide_EPS_from_Potato_Rhizosphere_in_Highland_and_the_Potential_as_a_Producer_Indole_Acetic_Acid_IAA

Nur, F. (2013). Fitoremediasi Logam Berat Kadmium (Cd). Biogenesis, 1 (1):74-83. http://journal.uin-alauddin.ac.id/index.php/biogenesis/article/viewFile/450/427

Nurhayati, D. & Didha, A. P. (2019). Bioakumulasi Logam Berat Pada Kerang Hijau (Perna viridis) di Perairan Cirebon Beradasarkan Musim yang Berbeda. Jurnal Akuatika Indonesia, 4 (1): 6-10. DOI:10.24198/jaki.v4i1.23484.

Palar, H. (2004). Pencemaran dan Toksikologi Logam Berat. Penerbit Rineka Cipta, Jakarta. ISBN: 9795185950.

Rinasari E, O., Maulana, Y. & Taufik, A. (2019). Kajian Pengelolaan Limbah B3 Hasil dari Kegiatan Pertambangan Batubara. Jurnal Pertambangan, 3 (4):52-58. https://ejournal.unsri.ac.id/index.php/mining/article/view/10013

Wawo, R.H.A., Sri, W., Nurliah, J. & Firman, N. Y. (2017). Analisis Pengaruh Penambangan Emas Terhadap Kondisi Tanah pada Pertambangan Rakyat Poboya Palu, Provinsi Sulawesi Tengah. Jurnal Geomine, 5 (3): 116-119. https://jurnal.teknologiindustriumi.ac.id/index.php/JG/article/view/141

Widyati E. (2007). The Use of Sulphate-Reducing Bacteria In Bioremediation of Ex-Coal Mining Soil. Biodiversitas, 8 (4):283-286. https://smujo.id/biodiv/article/view/427

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