Comparison of Anti-Bacterial Activity of Methanol Extract and Non-Polar Fraction of Macroalgae Sargassum sp.

Adi Tri Wibowo; Sri Widyastuti; Eka Sunarwidhi Prasedya; Neneng Rachmalia Izzatul Mukhlishah; Kukuh Waseso Jati Pangestu; Anggit Listyacahyani Sunarwidhi

doi:10.29303/jbt.v25i4.10522

Comparison of Anti-Bacterial Activity of Methanol Extract and Non-Polar Fraction of Macroalgae Sargassum sp.

Authors

Adi Tri Wibowo , Sri Widyastuti , Eka Sunarwidhi Prasedya , Neneng Rachmalia Izzatul Mukhlishah , Kukuh Waseso Jati Pangestu , Anggit Listyacahyani Sunarwidhi

DOI:

10.29303/jbt.v25i4.10522

Published:

2025-10-31

Issue:

Vol. 25 No. 4 (2025): Oktober-Desember

Keywords:

Anti-bacterial, FTIR, Non-polar fraction, Sargassum sp.

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Wibowo, A. T., Widyastuti, S., Prasedya, E. S., Mukhlishah, N. R. I., Pangestu, K. W. J., & Sunarwidhi, A. L. (2025). Comparison of Anti-Bacterial Activity of Methanol Extract and Non-Polar Fraction of Macroalgae Sargassum sp. Jurnal Biologi Tropis, 25(4), 6067–6075. https://doi.org/10.29303/jbt.v25i4.10522

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Abstract

Sargassum sp. is an abundant source of bioactive non-polar compounds, however their anti-bacterial potential remains understudied. This study aimed to compare the anti-bacterial activity between the methanol extract and non-polar fraction of Sargassum sp. Extraction was performed using sonication with methanol, followed by partition with petroleum ether and fractionation on silica gel G60 using n-hexane:ethyl acetate (9:1). FTIR analysis characterized the functional groups, while anti-bacterial evaluation was performed using the disc diffusion method against Staphylococcus aureus. Data were analyzed statistically using one-way ANOVA. FTIR spectrum of the methanol extract showed absorption bands corresponding to non-polar compounds. In the non-polar fraction, the bands were more dominant, suggesting a higher content of non-polar compounds. Anti-bacterial evaluation of Sargassum sp., methanol extract and non-polar fraction exhibited inhibitory effects against S. aureus. The non-polar fraction produced a larger inhibition zone compared to the methanol extract, indicating stronger anti-bacterial activity and significant difference between the two samples (p<0.05). The results of this study showed that the non-polar fraction of Sargassum sp. methanol extract has higher anti-bacterial activity compared to the extract. Further studies are recommended to elucidate the individual bioactive compounds and identify their anti-bacterial mechanisms.

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Comparison of Anti-Bacterial Activity of Methanol Extract and Non-Polar Fraction of Macroalgae Sargassum sp.

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