The Effect of Yeast, NPK and Fermentation Time in Bioethanol Fermentation from “Abacaxi” Pinapple Pericarp

Authors

Nery Sofiyanti , Dyah Iriani , Putri Intan Wahyuni , Nurul Idani , Puji Lestari

DOI:

10.29303/jbt.v22i4.4312

Published:

2022-11-16

Downloads

Abstract

Bioethanol is promising alternative fuel due to its less effect to environment. It is produced from bio-sources, such plant materials. Yeast and NPK have been reported as the ingredients in affecting bioethanol fermentation. The aim of this study was to examined the effect of yeast and NPK in bioethanol fermentation using the pericarp of “Abacaxi” pinnapple cultivar (Ananas comosus cv. “Abacaxi”). The pericarp juice of “Abacaxi” pineapple was made by blending the fresh pericarp and aquadest (1 : 1.5). The juice was then mix with dry commercial yeast (1 g, 2.5 g and 5 gr) and NPK  (1 g, 2 g, 3 g). A total of 9 treatments were used in fermentation.  Each treatment was replicated three times, brought the total sample was 27. The percentage of bioethanol for each treatment was measure using alcoholmeter for six days (24 h, 48 h, 72 h, 96 h, 120 h and 144 h). The result show that the percentage of bioethanol produced in this study varies among different treatment and fermentation time. Yeast and NPK gave significant effect in bioethanol fermentation, with the combination of 5 g yeast and 3 g NPK gave the highest percentage of bioethanol in 144 h of fermentation. Based on the result of this study, the pericarp of “Abacaxi” pineapple is potential bio-source for bioethanol fermentation.

Keywords:

abacaxi bioethanol fermentation pineapple pericarp

References

Albergarian M., & Arneborg, N. (2016). Dominance of Saccharomyces cerevisiae in alcoholic fermentation process: role of physiological fitness and microbial interactions. Applied Microbiology and Biotechnology 100(5). 2035 – 2046. DOI:10.1007/s00253-015-7255-0

Antonio, R. M. D. R., Cruz, A. A. C, Quinto Jr. A. S, Cordero, P. R., & Dimaano M. N. R. (2015). Bioethanol Fermentation from Pineapple (Ananas comosus) Peelings using Saccharomyces cerevisiae as Fermenting Yeast with Focus on Fermentation pH. International Journal of Engineering Research & Technology 4(5): 356 – 340.

Chouaibi, M., Daoued, K. B., Riguane, K., Rouissi, T., & Ferrari, G., (2020). Fermentation of bioethanol from pumpkin peel wastes: Comparison between response surface methodology (RSM) and artificial neural networks (ANN), Industrial Crops and Products 155. DOI: https://doi.org/10.1016/j.indcrop.2020.112822.

Christofi, S., Papanikolaou, S., Dimopoulou, M., Terpou, A., Cioroiu, I.B. Cotea, V. & Kallithraka, S., (2022). Effect of Yeast Assimilable Nitrogen Content on Fermentation Kinetics, Wine Chemical Composition and Sensory Character in the Fermentation of Assyrtiko Wines. Appl. Sci. 72(1405): 1-18. . https://doi.org/10.3390/ app12031405

Ciani, M., Comitinil, F., & Mannazzu, I. (2008). Fermentation. Encyclopedia of Ecology: 1548-1557. Science Direct. https://doi.org/10.1016/B978-008045405-4.00272-X

Cordenuns, Saura-Calixto, F., Diaz-Rubio, M., Zuleta, A., Tiné, M., Buckeridge, M. S., Silva, B., Carpio, C., Giuntini, E., Menezes, E., & Lajolo, F. (2010). Carbohydrate composition of ripe pineapple (cv. perola) and the glycemic response in humans. Ciênc. Tecnol. Aliment, Campinas 30(1): 282-288.

Edeh, I. (2020). Bioethanol Fermentation: An Overview. In Bioethanol Technology. 10.5772/intechopen.94895.

Fitria, N., & Lindasari, E. (2021). Optimasi Perolehan Bioetanol dari Kulit Nanas (Ananas cosmosus) dengan Penambahan Urea, Variasi Konsentrasi Inokulasi Starter dan Waktu Fermentasi. Reka lingkungan 9(1): 1 – 10.

Hastuti, E.D., Prihastanti, E., & Haryanti, S. (2015) Efektifitas Penambahan Ragi Dan Pupuk Terhadap Kadar Alkohol Bioetanol Dengan Bahan Baku Jambu Citra. Bulletin anatomi dan Fisiologi XIII (1): 92 – 99. https://doi.org/10.14710/baf.v23i1.8739

Hilma, R., Akbar, U., & Prasetya. (2017). Optimum Condition of Bioetanol Fermentation Via Acidic Hydrolysis From Pineaple (Ananas comosus Merr.) Peel Waste In Kualu Village-Kampar. Photon: Jurnal Sain Dan Kesehatan 7(02): 135-142. https://doi.org/10.37859/jp.v7i02.519.

Jalil, N., & Hossain, N. (2015). Sugar and Bioethanol Fermentation from Oil Palm Trunk (OPT). Asia Pacific Journal of Energy and Environment 2(2): 89-92. DOI:10.18034/apjee.v4i1.237

Joy, P. P., & Anjana, R. (2013). Pineapple Varieties. https://www.researchgate.net/publication/306034709_PINEAPPLE_VARIETIES.

Khalid, A. D., Chafidz, A., Lukman, M. A., & Kholil, I. (2019). Making of bioethanol banana weevil as renewable energy. IOP Conference Series: Materials Science and Engineering, Volume 722, 3rd International Conference on Engineering Technology for Sustainable Development (ICET4SD) 23–24 October 2019, Yogyakarta, IndonesiaCitation Ab D Khaliq et al 2020 IOP Conf. Ser.: Mater. Sci. Eng. 722 0 12080

Kim, H. (2015). Statistical notes for clinical researchers: post-hoc multiple Comparisons. Restor Dent Endod, 40(2):172-176. https://doi.org/10.5395/rde.2015.40.2.172

Malakar, S., Paul, S. K., & Pou, K. R. J. (2020). Biotechnological Interventions in Beverage Fermentation. Biotechnological Progess and Beverage Consumption 19: 1 – 37. https://doi.org/10.1016/B978-0-12-816678-9.00001-1

Mandari, S., Yenie, S., & Muria S. R., (2022). Pembuatan Bioetanol dari Kulit Nanas (Ananas comosus L.) Menggunakan Enzim Selulase dan Yeast Saccharomyces Cerevisiae dengan Proses Simultaneous Sacharificatian and Fermentation (SSF). https://media.neliti.com/media/publications/201549-pembuatan-bioetanol-dari-kulit-nanas-ana.pdf (August 9th, 2022)

Moshi, A. K., Hosea, K., Emrode, E., Mshandete, A., & Nges, I. (2014). Fermentation of Bioethanol from Wild Cassava Manihot glaziovii through Various Combinations of Hydrolysis and Fermentation in Stirred Tank Bioreactors. British Biotechnology Journal 5. 10.9734/BBJ/2015/13981.

Muktar, M. (2013). Evaluation of Sugar Content and Bioethanol Potentials of Some Freshwater Biomass. International Journal of Renewable and Sustainable Energy 2(6): 201 – 216.. DOI: 2. 201. 10.11648/j.ijrse.20130206.12.

Myers, N., Mittermeier, R. A., Mittermeier, C. G., Fonseca, G. A. B., & Kent, J. (2000). Biodiversity hotspots for conservation priorities. Nature 403(2): 853–858

Nahak, S, Nahak, G., Pradhan, I., & Sahu, R. (2011). Bioethanol from Marine Algae: A Solution to Global Warming Problem. Journal of Applied Environmental and Biological Sciences 1: 74-80.

Okonkwo, S. I., & Uyo, B.K. (2012). Elucidation of Sugar in Edible Fruit – Pineapple (Ananas comosus). Research Journal of Chemical Sciences 2(1): 20 – 24.

Setyawati, I., Ambarsari, L., Nur’aeni, S., Suryani, Puspita, P. J., Kurniatin, P. A., & Nurcholis, W. (2015). Bioethanol Fermentation by Using Detoxified Sugarcane Bagasse Hydrolysate and Adapted Culture of Candida tropicalis. Current Biology 2 (1): 1 – 12.

Susilowati, D., Subekti, N., & Bintari, S. H., (2018). The Potential of Microbial Symbionts Macrotermes gilvus Hagen Termite Gut as Degrading Agents of Cellulose in Bioethanol Production. Biosaintifika Journal of Biology and biology Education 10(2): 395 – 400. http://dx.doi.org/10.15294/biosaintifika.v10i2.14965

Teigen, K. H. (2010). Post Hoc Probability Judgements and Counterfactual Closeness 4(2): 147-177 | https://doi.org/10.1080/135467898394193.

Tesfaw, A., & Assefa, F. (2014). Review Article Current Trends in Bioethanol Fermentation by Saccharomyces cerevisiae: Substrate, Inhibitor Reduction, Gowth Variables, Coculture, and Immobilization. International Scholarly Research Notices 2014: 1 – 12. DOI:http://dx.doi.org/10.1155/2014/532852

Tse, T. J., Wiens, D. J., & Reaney, M. T. J. (2021). Fermentation of Bioethanol—A Review of Factors Affecting Ethanol Yield. Fermentation 7(268): 1 – 18. DOI: https://doi.org/ 10.3390/fermentation7040268

Utomo, W., & Palupi, A. E. (2013). Pengaruh Penambahan Pupuk Npk Pada Fermentasi Umbi Ganyong (Canna edulis Kerr) Untuk Menghasilkan Bioetanol Sebagai Extender Premium. JTM 2(2): 8-15.

Walker, G. M., & Stewart, G. G. (2016). Saccharomyces cerevisiae in the Fermentation of Fermented Beverages Beverage 2(30): 1 – 12. Doi: 10.3390/beverages2040030

Zabed, A. M., Faruq, G., Zahu, J. N., & Azirun, M. S. (2014). Bioethanol Fermentation from Fermentable Sugar Juice. The Scientific World Journal 2014: 1 – 11. DOI:10.1155/2014/957102

Zabochnicka-Świątek, M., & Sławik, L. (2010). Bioethanol-Fermentation and Utilization. Archivum Combustionis 30(3): 230 – 246.

Zentou, H., Abidin, Z. Z., Yunus, R., Biak, D. R. A, Issa, M. A., & Pudza, M. Y. (2021). A New Model of Alcoholic Fermentation under a Byproduct Inhibitory Effect. ACS Omega 6(6): 4137–4146. https://doi.org/10.1021/acsomega.0c04025

Author Biographies

Nery Sofiyanti, Universitas Riau

Author Origin : Indonesia

Dyah Iriani, Universitas Riau

Author Origin : Indonesia

Putri Intan Wahyuni, Universitas Riau

Author Origin : Indonesia

Nurul Idani, Universitas Riau

Author Origin : Indonesia

Puji Lestari, Universitas Riau

Author Origin : Indonesia

Downloads

Download data is not yet available.

How to Cite

Sofiyanti, N., Iriani, D. ., Wahyuni, P. I., Idani, N., & Lestari, P. (2022). The Effect of Yeast, NPK and Fermentation Time in Bioethanol Fermentation from “Abacaxi” Pinapple Pericarp. Jurnal Biologi Tropis, 22(4), 1132–1138. https://doi.org/10.29303/jbt.v22i4.4312

Most read articles by the same author(s)

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.