Acute toxicity test of anthelmintic herbal on Artemia salina larvae
DOI:
10.29303/jpm.v18i5.5094Published:
2023-09-30Downloads
Abstract
Jamu X is a traditional medicine that is believed to provide anti-inflammatory effects, contains natural ingredients such as Curcuma xanthorrhiza Robx and Curcuma aeruginaosa Robx, has long been used to treat worm infections in children, but it has not passed preclinical tests. The use of traditional medicine in Indonesia has also increased to 44.3%, 55.3% in liquid form, and the rest in powder form. Therefore, this study aimed to determine the potential acute toxicity of Jamu X to Artemia salina larvae presented as its Furthermore, to determine the damaged parts of the larval organs using the Brine Shrimp Lethality Test (BSLT) method. This experimental study used 10000, 1000, 100, 10, 1, and 0.1 ppm, and each concentration contained 10 Artemia salina larvae. Then, we obtained the upper limit of 10,000 and the lower limit of 1000. In the following test, the larvae were grouped into six groups consisting of 10 Artemia salina larvae, and each group was given Jamu "X" solution with a series of concentrations 10000, 7820, 5640, .168, 1780, and 1000 ppm. Mortality data of Artemia salina larvae were analyzed by probit analysis to determine the value of . The results of this study showed the value was 1548.81 µg/mL, and damage was found in the thorax, antennae, antennule, stigma, and abdomen. It shows that Jamu X does not have the potential for acute toxicity or is non-toxic, which is indicated by the value of > 1000 ppm.
Keywords:
Jamu, Toxicity, Artemia salinaReferences
Kusumo, A. R., Wijoyo, F. Y., Haekal, P. P., Khairunnisa, I., Suhandi, R. I., & Prastika, S. S. (2020). Jamu Tradisional Indonesia: Tingkatkan Imunitas Tubuh Secara Alami Selama Pandemi Traditional. Jurnal Layanan Masyarakat, 4(2), 465–471.
Andriati, & Wahyudi, R, M. T. (2016). Tingkat penerimaan penggunaan jamu sebagai alternatif penggunaan obat modern pada masyarakat ekonomi rendah-menengah dan atas. Jurnal Masyarakat, Kebudayaan Dan Politik, 29(3), 133–145.
Riyan, R. D., Nahdliyyati, D., Salsabillah, T. A., Pramesti, A. A. S. D., Salsabila, F., Ramdanti, F., Putri, M. A. E., Jayalalitha, D. M., Nugrahesi, R., Setiawan, R., Hidayati, D. N., Dewi, G. D. P. K., Ayudya, J. R., Syayidah, E. E., & Puspitasari, H. P. (2022). Pengetahuan Masyarakat terhadap Penggunaan Jamu untuk Meningkatkan Imunitas Penderita COVID-19 yang Pernah Menjalani Isolasi Mandiri. 9(2), 194–199.
Handajani, sih rini, & Widhiastuti, K. endah. (2019). Budaya Pemberian Jamu Cekok Terhadap Peningkatan Rerata Berat Badan Batita Di Wilayah Kota Surakarta. Jurnal Ilmiah Bidan, IV(1), 1–8.
Rahimah, S., Dzulkifli, Suwahyuni, Aksa, R., Salampe, M., & Awaluddin, A. (2021). Uji Toksisitas Subakut Ekstrak Etanol Daun Asam Jawa Dosis Efektif Sebagai Antihiperglikemia Terhadap Kadar AST, ALT, dan Kreatinin Tikus (Rattus norvegicus). Jurnal Farmasi Indonesia, 18(1), 25–31.
Sakinah, A., Muhaimin, & Yuliawati. (2022). Uji Toksisitas Akut Ekstrak Etanol Daun Sungkai (Peronema canescens Jack) Terhadap Fungsi Ginjal Mencit Putih Betina (Mus musculus Linn.). Jurnal Ilmu Farmasi Dan Farmasi Klinik (JIFFK), 19(2), 54–62.
Jannah, D. W., Maunatin, A., & Jannah, A. (2020). Identifikasi dan Uji Toksisitas terhadap Larva Udang (Artemia salina L.) Ekstrak Bekatul Menggunakan Variasi Jenis Pelarut dan Lama Ekstraksi Dewi. 8(2), 16–23.
Nofita, N., Maria Ulfa, A., & Delima, M. (2020). Uji Toksisitas Ekstrak Etanol Daun Jambu Biji Australia (Psidium Guajava L) Dengan Metode Bslt (Brine Shrimp Lethality Test). JFL: Jurnal Farmasi Lampung, 9(1), 10–17.
Zulfiah, Z., Megawati, M., Herman, H., H. Ambo Lau, S., Hasyim, M. F., Murniati, M., Roosevelt, A., Kadang, Y. K., AR, N. I., & Patandung, G. (2020). Uji Toksisitas Ekstrak Rimpang Temu Hitam (Curcuma aeruginosa Roxb.) Terhadap Larva Udang (Artemia salina Leach) dengan Metode Brine Shrimp Lethality Test (BSLT). Jurnal Farmasi Sandi Karsa, 6(1), 44–49.
Tyas, N. M., Batu, D. T. F. L., & Affandi, R. (2016). The lethal toxicity test of Cr6+ on (Oreochromis niloticus). Jurnal Ilmu Pertanian Indonesia, 21(2), 128–132.
Lazuardi, M. (2016). Bagian Umum Ilmu Farmasi Veteriner. Jakarta: Ghalia Indonesia.
Meyer, B.N., Ferrigni, N.R., Putnam, J.E., Jacobsen, L.B., Nichols, D.E., McLaughlin, J.L., 1982. Brine Shrimp: A Convenient General Bioassay For Active Plant Constituents. Planta Medica. 45: 31-34.
Naqinie, Maisy, Erida Wydiamala, and Lisda Hayatie. n.d. “Uji Aktivitas Ekstrak Etanol Daun Water Mimosa ( Neptunia oleracea L ) Sebagai Larvasida Terhadap Nyamuk Aedes aegypti.” 593–602.
Caroline, Jenny, Rachmanu Eko Handriyono, Sonia S. Ximenes, and Maritha Nilam. 2019. “Analisis Tingkat Toksisitas Limbah Pewarnaan Jeans Menggunakan Ikan Nila (Oreochromis Niloticus).” Journal of Research and Technology 5(2):99–105.
Boyd, Claude E. (2005). LC50 Calculations Help Predict Toxicity. Global Aquaculture Alliance. 84-87
Mclaughlin, J. L., Rogers, L. L., & Anderson, J. E. (1998). The use of biological assays to evaluate botanicals. Therapeutic Innovation & Regulatory Science, 32(2), 513–524.
Clarkson, C., Maharaj, V. J., Crouch, N. R., Grace, O. M., Pillay, P., Matsabisa, M. G., Bhagwandin, N., Smith, P. J., & Folb, P. I. (2004). In vitro antiplasmodial activity of medicinal plants native to or naturalised in South Africa. Journal of Ethnopharmacology, 92(2–3), 177–191.
Ndruru, E, M.Situmorang, G. T. (2014). Analisa Faktor-Faktor Yang Mempengaruhi Hasil Produksi Padi di Deli Serdang Riang Enjelita Ndruru , Marihat Situmorang , Gim Tarigan. Saintia Matematika, 2(1), 71–83.
Raineri, M. (1981). Histochemical localization of chitin in larvae of Artemia salina Leach (Phyllopoda). Bolletino Di Zoologia, 48(2), 139–141.
Rahimah, S., Maryam, F. B., & Limbong, B. A. (2019). The Toxicity Test of Ethanol Extract of Leaves Averrhoa bilimbi L. Using Brine Shrimp Lethality Test (BSLT). Journal of Pharmaceutical and Medicinal Sciences, 4(1), 10–14
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