Chemical characteristics during the fermentation process of siam kintamani orange peel (Citrus nobilis) probiotic drink
DOI:
10.29303/jpm.v18i5.5613Published:
2023-09-30Issue:
Vol. 18 No. 5 (2023): September 2023Keywords:
Fermentation, Orange Peel, Probiotic Drink, Beneficial FoodArticles
Downloads
How to Cite
Downloads
Metrics
Abstract
Functional foods containing bacteria that may improve digestive function are called probiotic beverages. The fruit is rich in vitamins, minerals, antioxidants, and bioactive substances that benefit the body's health. Therefore, it can be used to make non-dairy probiotic drinks. Kintamani is a province in Bali that produces fruit, namely Siam oranges. In 2021, citrus fruit production in Kintamani, Bangli, is the second highest, at 104,528 per ton. However, there are still damaged fruits due to poor post-harvest processing and management. Orange peel is one of the citrus wastes that is not utilized, but this waste can still be processed into food with good nutritional value. The skin of Siam oranges contains secondary metabolites composed mainly of flavonoids, phenols, steroids, and triterpenoids. Furthermore, the Kintamani orange peel has better antioxidant potential than its seeds. Metabolites derived from Siam Kintamani orange peel have the potential to be used as probiotic beverages. A SCOBY (Symbiotic Culture of Bacteria and Yeasts) starter produces Siam orange peel probiotic drink. SCOBY is a gel layer formed from a mixture of bacteria and yeast. The desired outcome of this study was to assess the total acid, degree of acidity, pH, vitamin C, and total reducing sugar of Siam Kintamani orange peel as a result of fermented probiotic drinks. This is an experimental study with an entirely randomized approach. The treatment in this study was a variation in fermentation length, including several days (0, 3, 6, 9, 12, 15, and 18 days), with 4 repetitions. ANOVA was used to analyze the data, followed by a multiple-range test based on Duncan's equation, the fermentation time had a substantial impact on the total acid, pH, vitamin C, and total flavonoids of the Siam Kintamani orange peel probiotic drink (Citrus nobilis) (p<0.05). The time of the fermentation process influences the entire amount of acid, pH, vitamin C levels, and quantity of flavonoids in the Siam Kintamani orange peel probiotic drink. The longer the fermentation process, the more the total acid value increases while the pH decreases. The highest content of vitamin C and total flavonoids was produced on the ninth day of fermentation, at 70455.33 mg/100 ml and 25.46 mg QE/100 g, respectively.
References
Dewi, A. S., Atifah, Y., Farma, S. A., Yuniarti, E., & Fadhilla, R. (2021). Pentingnya Konsumsi Probiotik untuk Saluran Pencernaan dan Kaitannya dengan Sistem Kekebalan Tubuh Manusia. In Prosiding Seminar Nasional Biologi. 1 (1), 149-156.
Nagpal, R., Kumar, A., & Kumar, M. (2012). Fortification and fermentation of fruit juices with probiotic lactobacilli. Annals of microbiology, 62, 1573-1578.
Valero-Cases, E., Cerdá-Bernard, D., Pastor, J.J. and Frutos, M.J. (2020) Non-Dairy Fermented Beverages as Potential Carriers to Ensure Probiotics, Prebiotics, and Bioactive Compounds Arrival to the Gut and Their Health Benefits. Nutrients, 12, 666. https://doi.org/10.3390/nu12061666
BPS. 2021. Produksi Buah Jeruk Provinsi Bali Menurut Kabupaten/Kota (Ton) 2019-2021 [Internet]. [cited 30 March 2023]. Available from : https://bali.bps.go.id/indicator/55/200/1/produksi-buah-jeruk-provinsi-bali-menurut-kabupaten-kota.html
Kristiandi, K. and Sambas, P. N. (2020). Pemanfaatan kulit jeruk siam sebagai pestisida alami utilization of siam orange skin as a natural pesticide, 6(2), pp. 46–52.
Teknologi Pertanian Universitas udayana. (2022) [Internet]. [cited 30 March 2023]. Available from : https://www.unud.ac.id/in/berita-fakultas894-Tangani-Over-Produksi-Saat-Panen-Raya-Prodi-Teknologi-Pangan-FTP-Unud-Kembangkan-Inovasi-Pengolahan-Buah-Jeruk-Siam-Kintamani-Dengan-Model-Zero-Waste-Proses.html
Roos, J. D., & Vuyst, L. D. (2018). Acetic acid bacteria in fermented foods and beverages. Current Opinion in Biotechnology. 49, 115–119. https://doi.org/10.1016/j.copbio.2017.08.007
Marsh, A. J., O'Sullivan, O., Hill, C., Ross, R. P., & Cotter, P. D. (2014). Sequence-based analysis of the bacterial and fungal compositions of multiple Kombucha (tea fungus) samples. Food Microbiology, 38, 171–178. https://doi.org/10.1016/j.fm.2013.09.003
Coton, M., Pawtowski, A., Taminiau, B., Burgaud, G., Deniel, F., Coulloumme-Labarthe, L., & Coton, E. (2017). Unraveling microbial ecology of industrial-scale Kombucha fermentations by metabarcoding and culture-based methods. Fems Microbiology Ecology, 93(5), 1–16. https://doi.org/10.1093/femsec/fix048
Kusuma, G. P. A. W., Nocianitri, K. A., & Pratiwi, I. D. P. K. (2020). Pengaruh lama fermentasi terhadap karakteristik fermented rice drink sebagai minuman probiotik dengan isolat Lactobacillus sp. F213. Jurnal Itepa, 9(2), 181-192.
Hidaya, I. & Wikandari, P. R. (2020). Pengembangan Gelato Sinbiotik Berbahan Dasar Soygurt dan Umbi Gembili (Dioscorea esculenta L.). UNESA Journal of Chemistry, 9(1), pp. 17-22.
Vuong, Q. V., S. Hirun, T.L.K. Chuen, C.D. Goldsmith, M.C. Bowyer, A.C. Chalmers, P.A. Phillips dan C.J. Scarlett. (2014). Physicochemical composition, antioxidant and antiproliferative capacity of a lily pilly (Syzygium paniculatum) extract. Journal of Herbal Medicine. 10: 10- 16.
Singh, R., P.K. Verma, dan G. Singh. (2012). Total Phenolic, Flavonoids and Tannin Contents in Different Extracts of Artemisia Absinthium. J. Intercult. Ethnopharmacol. 1(2):101-104.
Pallant, Jullie. (2010). SPSS Survival Manual 4 th Edition. New York : Mc Graw Hill
Pratiwi, A., & Aryawati, R. (2012). Pengaruh waktu fermentasi terhadap sifat fisik dan kimia pada pembuatan minuman kombucha dari rumput laut Sargasssum sp. Maspari Journal: Marine Science Research, 4(1), 131-136.
Yunus, Y., & Zubaidah, E. (2015). Pengaruh Konsentrasi Sukrosa Dan Lama Fermentasi Terhadap Viabilitas L. Casei Selama Penyimpanan Beku Velva Pisang Ambon [In Press April 2015]. Jurnal Pangan dan Agroindustri, 3(2), 303-312.
Khotimah, K., & Kusnadi, J. (2014). Aktivitas Antibakteri Minuman Probiotik Sari Kurma (Phoenix dactilyfera L.) Menggunakan Lactobacillus plantarum dan Lactobacillus casei [IN PRESS JULI 2014]. Jurnal Pangan dan Agroindustri, 2(3), 110-120.
Kinteki, G. A., Rizqiati, H., & Hintono, A. (2019). Pengaruh lama fermentasi kefir susu kambing terhadap mutu hedonik, total bakteri asam laktat (BAL), total khamir dan pH. Jurnal Teknologi Pangan, 3(1), 42-50.
Greenwalt, C.J., Ledford, R.A., & Steinkraus, K.H. (1998). Determination and Characterization of The Antimicrobial Activity of The Fermented Tea Kombucha. Lebensm-Wiss. Technol. 31:291-296.
Pranayanti, I. A. P. dan A. Sutrisno. (2015). Pembuatan Minuman Probiotik Air Kelapa Muda (Cocos Nucifera L.) dengan Starter Lactobacillus casei strain Shirota. Jurnal Pangan dan Agroindustri, 3(2): 763-772.
Jayabalan, R., Malbaša, R. V., Lončar, E. S., Vitas, J. S., & Sathishkumar, M. (2014). A review on kombucha tea—microbiology, composition, fermentation, beneficial effects, toxicity, and tea fungus. Comprehensive reviews in food science and food safety, 13(4), 538-550.
Chakravorty, S., Bhattacharya, S., Chatzinotas, A., Chakraborty, W., Bhattacharya, D., & Gachhui, R. (2016). Kombucha tea fermentation: Microbial and biochemical dynamics. International journal of food microbiology, 220, 63-72.
Coelho, R. M. D., Almeida, A., do Amaral, R. Q. G., da Mota, R. N., & de Sousa, P. H. M. (2020). Kombucha: review. International Journal of Gastronomy and Food Science, 100272, 1-12. https://doi.org/10.1016/j.ijgfs.2020.100272.
Apriani, I. (2017). Pengaruh Proses Fermentasi Kombucha Daun Sirsak (Annona Muricata L.) Terhadap Kadar Vitamin C. Jurnal Biota, 3(2), 90-95.
Purnawati. (2011). Pengaruh Konsentrasi Ekstrak Buah Mengkudu pada Kombucha terhadap Kadar Vitamin C. Jurnal Muhamadiyah Surakarta.
Puspitasari, Y., Palupi, R., & Nurikasari, M. (2017). Analisis kandungan vitamin C teh kombucha berdasarkan lama fermentasi sebagai alternatif minuman untuk antioksidan. Global Health Science, 2(3), 245-253.
Author Biographies
Nadya Treesna Wulansari, Institut Teknologi dan Kesehatan Bali
A.A Istri Mas Padmiswari, Institut Teknologi dan Kesehatan Bali
Putu Rima Sintyadewi, Institut Teknologi dan Kesehatan Bali
License
Copyright (c) 2023 Nadya Treesna Wulansari, A.A Istri Mas Padmiswari, Putu Rima Sintyadewi
This work is licensed under a Creative Commons Attribution 4.0 International License.
The following terms apply to authors who publish in this journal:
1. Authors retain copyright and grant the journal first publication rights, with the work simultaneously licensed under a Creative Commons Attribution License 4.0 International License (CC-BY License) that allows others to share the work with an acknowledgment of the work's authorship and first publication in this journal.
2. Authors may enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., posting it to an institutional repository or publishing it in a book), acknowledging its initial publication in this journal.
3. Before and during the submission process, authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website), as this can lead to productive exchanges as well as earlier and greater citation of published work (See The Effect of Open Access).