Liver Histology of Male Rat (Rattus norvegicus) Feed L-Carnitine Supplement
DOI:
10.29303/jpm.v19i4.6900Published:
2024-07-29Issue:
Vol. 19 No. 4 (2024): July 2024Keywords:
L-carnitine; Liver; ObesityArticles
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Abstract
Obesity is a condition where the body is overweight due to fat accumulation, each exceeding 20-25% of body weight for men and women. L-carnitine is a nutrient that plays a role in transporting fat to the mitochondria to be burned and converted into energy for the body. Sufficient L-carnitine allows the body to burn fat at optimal levels. This research aims to determine the side effects of fed L-carnitine supplements against liver histology. This study used a completely randomized design (CRD) with four groups comprising six albino rats. The treatments were fed orally by distilled water 1 ml/day (Control), P1 fed by L-carnitine supplement dose 100 mg/kg body weight orally 1ml/day, P2 fed by L-carnitine supplement dose 150 mg/kg body weight orally 1ml/day, P3 fed by L-carnitine supplement dose 200 mg/kg body weight orally 1ml/day. Treatments were given to albino rats every day for 90 days. Variables observed measured were lipid degeneration, central vein dilation, and pyknotic nuclei. Data were analyzed with the statistical program (SPSS 22.0 for Windows) with One Way ANOVA. The results showed that feeding L-carnitine supplements in high doses for long periods can damage the liver cells of male rats. The microscopic liver damage statistical analysis showed significant differences (P<0.05) between the control and treatment groups.
References
ERCHO, N. C. (2014). Hubungan obesitas dengan kadar LDL dan HDL pada mahasiswa preklinik Fakultas Kedokteran Universitas Lampung Tahun 2013.
Pradana, A., Seno, K., & Puruhita, N. (2014). Hubungan antara indeks massa tubuh (IMT) dengan nilai lemak viseral (Studi kasus pada mahasiswa kedokteran Undip) (Doctoral dissertation, Faculty of Medicine Diponegoro University).
Hardani, E., Lestariana, W., & Susetyowati, S. (2014). Efek pemberian ekstrak teh hijau (Camellia sinesis (L) O. Kuntze) var. Assamica terhadap total lemak tubuh dan profil lipid wanita dewasa overweight dan obesitas. Jurnal gizi klinik indonesia, 10..
Caterson, I. D. (2009). Medical management of obesity and its complications. Ann Acad Med Singapore, 38(1), 22-7.
Theresa, A., Sukohar, A., & Wardhana, M. F. (2023). Nutrasetikal dalam Pengelolaan Obesitas. Medical Profession Journal of Lampung, 13(4), 472-478.
Kalpana, A., & Aruna, D. (2012). Effects of l-carnitine [Neutraceutical] in weight management among overweight and obese adults of age between 20–45 yrs—A comparative study in Chennai and Tirupathi. Int J Sci Res Publ, 2, 1-5.
Pooyandjoo, M., Nouhi, M., Shab‐Bidar, S., Djafarian, K., & Olyaeemanesh, A. (2016). The effect of (L‐) carnitine on weight loss in adults: a systematic review and meta‐analysis of randomized controlled trials. Obesity reviews, 17(10), 970-976.
Wall, B. T., Stephens, F. B., Constantin‐Teodosiu, D., Marimuthu, K., Macdonald, I. A., & Greenhaff, P. L. (2011). Chronic oral ingestion of L‐carnitine and carbohydrate increases muscle carnitine content and alters muscle fuel metabolism during exercise in humans. The Journal of physiology, 589(4), 963-973.
Stephens, F. B., Constantin‐Teodosiu, D., Laithwaite, D., Simpson, E. J., & Greenhaff, P. L. (2006). Insulin stimulates L‐carnitine accumulation in human skeletal muscle. The FASEB journal, 20(2), 377-379.
Sherwood, L. (2020). Fisiologi manusia dari sel ke sistem.
Utomo, Y., Hidayat, A., Dafip, M., & Sasi, F. A. (2012). Studi histopatologi hati mencit (Mus musculus L.) yang diinduksi pemanis buatan. Indonesian Journal of Mathematics and Natural Sciences, 35(2).
Setiani, N. N. G., Loho, L., & Lintong, P. (2016). Gambaran histopatologik hati tikus wistar (Rattus norveginus) yang diinduksi monosodium glutamate (msg) dan diberikan sari air bawang daun (Allium fistulosum L.). eBiomedik, 4(2).
Al-Daraji, H. J., & Tahir, A. O. (2014). Effects of dietary L-carnitine supplementation on testicular histology of Iraqi drakes.
Rahmi, H. (2009). Studi hematologis dan histopatologis organ pada tikus yang diinduksi kuinin sebagai uji potensi metabolik angkak. Skripsi. Institut Pertanian Bogor: Fakultas Matematika dan Ilmu Pengetahuan Alam. Departemen Biokimia.
Muthiadin, C., Zulkarnain, Z., & Hidayat, A. S. (2020). Pengaruh pemberian tuak terhadap gambaran histopatologi hati mencit (Mus musculus) ICR jantan. Jurnal Pendidikan Matematika dan IPA, 11(2), 193-205.
Pramesti, N. K. T., Wiratmini, N. I., & Astiti, N. P. A. (2017). Struktur Histologi Hati Mencit (Mus musculus L.) Setelah Pemberian Ekstrak Daun Ekor Naga (Rhapidhophora pinnata Schott). Jurnal Simbiosis, 2, 43-46.
Setiani, N. N. G., Loho, L., & Lintong, P. (2016). Gambaran histopatologik hati tikus wistar (Rattus norveginus) yang diinduksi monosodium glutamate (msg) dan diberikan sari air bawang daun (Allium fistulosum L.). eBiomedik, 4(2).
Taylor, S. A., & Miloh, T. (2018). Adolescent Alcoholic Liver. Alcoholic Liver Disease, An Issue of Clinics in Liver Disease, 23(1), 51-54.
Budi Pramono, Y. (2022). Buku Monograf: Mengapa Belum Mendapat Keturunan? Pola Makan dan Pola Hidup Sehat untuk Mendapatkan Keturunan.
Madihah, R. N., Malini, D. M., Faiza, A. H., & Iskandar, J. (2017). Uji toksisitas akut ekstrak etanol kulit buah jengkol (Archidendron pauciflorum) terhadap tikus Wistar betina. Prom Sem Nas Masy Biodiv Indon, 3, 33-8.
Hoehme, S., Brulport, M., Bauer, A., Bedawy, E., Schormann, W., Hermes, M., ... & Drasdo, D. (2010). Prediction and validation of cell alignment along microvessels as order principle to restore tissue architecture in liver regeneration. Proceedings of the National Academy of Sciences, 107(23), 10371-10376.
Singh, K. A. R. A. M., & Verma, B. H. A. V. N. A. (2012). Ayurvedic perspectives towards Cerebral palsy. Journal of Research and Education in Indian Medicine, 18(3-4), 163-74.
Simanjuntak, S. J. O., Berata, I. K., Winaya, I. B. O., Setiasih, N. L. E., Sudimartini, L. M., & Susari, N. N. W. (2024). The Effect Of Mimosine From Simplicia of The Lamtoro Leaf on the Histopathological Of White Rats’testis. Buletin Veteriner Udayana, 851-860.
Amalina, N. (2009). Uji toksisitas akut ekstrak Valerian (Valeriana officinalis) terhadap hepar Mencit Balb/C (Doctoral dissertation, Medical faculty).
Flanagan, J. L., Simmons, P. A., Vehige, J., Willcox, M. D., & Garrett, Q. (2010). Role of carnitine in disease. Nutrition & metabolism, 7, 1-14.
Author Biographies
Anak Agung Istri Mas Padmiswari, Bachelor of Applied Acupuncture and Herbal Medicine Study Program, Faculty of Health, Institute of Technology and Health Bali
Putu Indrayoni, Bachelor of Clinic and Community Pharmacy Program, Faculty of Health, Institute of Technology and Health Bali
Nadya Treesna Wulansari, Bachelor of Food Technology Program, Faculty of Technology, Institute of Technology and Health Bali
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Copyright (c) 2024 Anak Agung Istri Mas Padmiswari, Putu Indrayoni, Nadya Treesna Wulansari
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