Isolate and Characterization of Lactic Acid Bacteria (LAB) in Local Nira as Probiotic Starter Candidates
DOI:
10.29303/jbt.v22i4.4429Published:
2022-11-29Downloads
Abstract
Probiotics are dietary supplements that contain lactic acid bacteria (BAL), which are capable of converting sugars (including lactose). Probiotics are used in the prevention of infectious diseases. Nira is often used as an ingredient to make alcoholic beverages (tuak) and sugar, but it has not been able to increase the economic value of sap water. The purpose of this study was to isolate lactic acid bacteria in sap as a probiotic candidate. The method in this study was carried out by isolating lactic acid bacteria in sap with a dilution series of 10-0, 10-1, 10-2, 10-3, 10-4, 10-5, and 10-6, then pipetting 1 ml into a cup petri dish and incubating in an anaerobic jar equipped with Anaerocult for 2 x 24 hours. Then macroscopic and microscopic observations were made and sugar and catalase tests were carried out to determine the type of bacteria found. The results of the isolation and characterization of bacteria in the sap were found to be 4 isolates, namely isolate 1A, which is Bacillus coagulans bacteria. Isolate 1B was a Bacillus licheniformis bacteria. Isolates 2A and 2B are bacteria from the genus Lactobacillussp.
Keywords:
Isolation, characterization, lactic acid bacteria, Nira, probioticsReferences
Afrianti Rahayu, S., & Muhammad Hidayat Gumilar, M. (2017). Uji Cemaran Air Minum Masyarakat Sekitar Margahayu Raya Bandung Dengan Identifikasi Bakteri Escherichia coli. Indonesian Journal of Pharmaceutical Science and Technology, 4(2), 50
Akhdiya A. (2003). Isolasi Bakteri Penghasil Enzim Protease Alkalin Termostabil Buletin Plasma Nutfah, 9 (2): 38-44.
Asan Özüsaglam, M. (2010). Importance of Bacillus coagulans Bacterium as Probiotic in Animal Nutrition. Süleyman Demirel Üniv. Ziraat Fak. Derg. 2010, 5, 50–57.
Baharuddin, Muin M, & Bandaso H. (2007). Pemanfaatan Nira Aren (Arenga pinnata Merr) sebagai Bahan Pembuatan Gula Putih Kristal. Jurnal Perennial, 3(2): 40-43
Cowan and Stell’s. (1973). Manual for the Identification of Medical Bacteria. Cambridge University Press Englan
Djide, M.N. & E. Wahyuddin (2008). Isolasi bakteri asam laktat dari air susu ibu dan potensinya menurunkan kadar kolesterol secara in-vitro. Majalah Farmasi dan Farmakologi. 12(3):73-78.
Djide, MN, & Sartini (2008). Dasar-Dasar Mikrobiologi Teknologi Laboratorium Kesehatan. Makassar: Fakultas Farmasi Universitas Hasanuddin
FAO/WHO. (2001). Joint FAO/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria. Amerian Córdoba Park Hotel, Córdoba, Argentina.
Fitri, L. & Y. Yasmin (2011). Isolasi dan pengamatan morfologi koloni bakteri kitinolitik. Jurnal Ilmiah Pendidikan Biologi. 3(2):20-25.
Glaser, R.; & Venus, J. (2018). Co-fermentation of the main sugar types from a beechwood organosolv hydrolysate by several strains of Bacillus coagulans results in effective lactic acid production. Biotechnol. Rep. 2018, 18, e00245.
Hardiningsih, R., R.N.R. Napitupulu, & T. Yulinery (2006). Isolasi dan uji resistens beberapa isolat Lactobacillus pada pH rendah. Biodiversitas. 7(1): 15-17.
Hirayama K, & Rafter J, (1999). The role of lactic acid in prevention of colon cancer: mechanistic consideration. Ann van Leewenhoek, 76: 391-394.
Isolauri E, Rautava S, Kalliomaki M, Kirjavainen P, & Salminen S. (2002). Role of probiotics in food hypersensitivity. Curr Opin Allergy Clin Immunol 2: 263-267
Jiang, T.; Qiao, H.; Zheng, Z.; Chu, Q.; Li, X.; Yong, Q.; & Ouyang, J. (2016). Lactic acid production from pretreated hydrolysates of corn stover by a newly developed Bacillus coagulans strain. PLoS ONE 2016, 11, e0149101.
Kalliomaki M, Salminne S, Pussa T, Arvilommi H, & Isolauri E. (2003). Probiotics and prevention of atopic disease: a randomized placebo-conrolled trial. Lancet 61: 1869-1871.
Karri, S.K.; Majeed, M.; Natarajan, S.; Sivakumar, A.; Ali, F.; Pande, A.; & Majeed, S. (2016). Evaluation of anti-diarrhoeal activity of Bacillus coagulans MTCC 5856 and its effect on gastrointestinal motilıty in wistar rats. Int. J. Pharm. Biol. Sci., 7, 311–316.
Keynan, A. & N. Sandler (1983). The Bacterial Spore, vol 2. (Hurst, A. and Gould, G. W., eds). Academic Press, New York: 107 pp
Koneman, E.W. (2006). Koneman’s Color Atlas and Textbook of Diagnostic Microbiology. Lippincott Williams & Wilkins, USA.
Marteau PR, de Vrese M, Cellier CJ, & Schrezeenmeier J. (2001). Protection from gastrointestinal diseases with the use of probiotics. Am J Clin Nutr 73: 430S-436S.
Milles, D. R. (1993). Manipulation of the Microflora of the Gastrointestinal Trac Natural Ways to Prevent Colonization by Pathogen in Biotec in the Feed Industry. Proc. of Altech's Ninth Annual Symp. Altech Technic. Publ. 3031 catnip Hill Pike, Nicholasvile, Kentucky 40356
Naiola, E. (2008). Mikrobia amilolitik pada nira dan laru dari Pulau Timor, Nusa Tenggara Timur. Biodiversitas. Volume 3 (3): 165-168.
Oxoid (1982). The Oxoid mannual of Culture Media, Ingredients and Other Laboratory Services. 5th ed. Basingtoke, Hampshire.
Prado, F. C., J. L. Parada, A. Pandey, & C. R. Soccol. (2008). Trends in non-dairy probiotic beverages. Food Res. Int. 41: 111-123
Purwani, E., S.W.N. Hapsari, & R. Rauf. (2009). Respon hambatan bakteri gram positif dan negatif pada ikan nila (Oreochromis niloticus) yang diawetkan dengan ekstrak jahe (Zingiber officinale). Jurnal Kesehatan. 2(1): 61-70.
Puspita, F., Muhammad, A., & Ridho, P. (2017). Isolasi dan Karakterisasi Morfologi dan Fisiologi Bakteri Bacillus sp. Endofitik dari Tanaman Kelapa Sawit (Elaeis guineensis Jacq.). J. Agrotek. Trop, 6(2), 44–49.
Putra, Y.S. (2015). Isolasi Bakteri Asam Laktat (BAL) pada Feses Orangutan Sumatera (Pongo abelii) di Kebun Binatang Bukittinggi Sumatera Barat. Skripsi. Fakultas Kedokteran Hewan, Universitas Syiah Kuala, Banda Aceh
Putri, A. L., & Kusdiyantini, E. (2018). Isolasi dan identifikasi bakteri asam laktat dari pangan fermentasi berbasis ikan (Inasua) yang diperjualbelikan di Maluku-Indonesia. Jurnal Biologi Tropika, 1(2), 6-12.
Rahayu, K. (1990). Enzim Mikroba. Departemen Pendidikan dan Kebudayaan, PAU Pangan dan Gizi. Institut Pertanian Bogor, Bogor: 108 hal.
Reid G, Howard J, & Gan BS. (2001). Can bacterial interference prevent infection?. Trends in Microbiol 9: 424-427.
Rostinawati, T., & Lestari, H. S. (2017). Skrining Bakteri Penghasil Enzim β -Siklodekstrin Transferase (β -CGTase) dari Tanah Jatinangor Glukosil. Jurnal Farmasi, Sains, dan Kesehatan, 3(2), 10–17.
Sardiani, N., Litaay, M., Budji, R. G., Priosambodo, D., Syahribulan, & Dwyana, Z. (2015). Potensi Tunikata Rhopaleae sp. sebagai sumber inokulum bakteri endosimbion penghasil antibakteri: 1. karakteristik isolat. Jurnal Alam Dan Lingkungan, 6(11)
Veith, B., Herzberg, c., Steckel, S., Feesche, J., Maurer, K. H., Ehrenreich, P., Baumer, S., Henne, A., Liesegang, H., Merkl, R., Ehrenreich, A, & Gottschalk, G. (2004). The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential. J Mol. Microbiol. Biotechnol, 7(4):204-2l1
Wizna, H. Abbas, Y Rizal, A. Dharma, & P. Kompiang (2013). Potensi Bacillus coagulans dari Serasah Hutan sebagai Probiotik Ayam Broiler. Jurnal Peternakan Indonesia, 15 (1): 75-80.
Yuniastuti, A. (2014). Buku Monografi Probiotik (Dalam Perspektif Kesehatan). Cetakan 1, UNNES Press, Semarang. ISBN: 9786022850038, pp: 94
License
Copyright (c) 2022 Aini Aini, Jumari Ustiawaty, Edy Kurniawan, Alfin Maulana
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.
