Uji Antiinflamasi Ekstrak Etanol Ashitaba (Angelica keiskei) Secara In Vitro

Mariama Fitriana; Wahida Hajrin; Windah Anugrah Subaidah; Sucilawaty Ridwan; Eskarani Tri Pratiwi

doi:10.29303/jbt.v24i2b.8105

Uji Antiinflamasi Ekstrak Etanol Ashitaba (Angelica keiskei) Secara In Vitro

Authors

Mariama Fitriana , Wahida Hajrin , Windah Anugrah Subaidah , Sucilawaty Ridwan , Eskarani Tri Pratiwi

DOI:

10.29303/jbt.v24i2b.8105

Published:

2024-12-26

Issue:

Vol. 24 No. 2b (2024): Special Issue

Keywords:

Angelica keiskei; anti-inflammatory effect; percent of inhibition; protein denaturation.

Articles

Downloads

pdf

How to Cite

Fitriana, M., Hajrin, W., Subaidah, W. A., Ridwan, S., & Pratiwi, E. T. (2024). Uji Antiinflamasi Ekstrak Etanol Ashitaba (Angelica keiskei) Secara In Vitro. Jurnal Biologi Tropis, 24(2b), 239–247. https://doi.org/10.29303/jbt.v24i2b.8105

Download Citation

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Abstract

Angelica keiskei has potential as an anti-inflammatory agent because it contains chalcone which has been proven to be able to suppress inflammation through inhibiting nitric oxide production and inhibiting the expression of the iNOS and COX-2. The anti-inflammatory potential of Angelica keiskei needs to be tested. This study aimed to determine the anti-inflammatory activity of Angelica keiskei ethanol extract using the protein denaturation inhibition method. Angelica keiskei was extracted using the sonication method with 96% ethanol. The extract was tested for anti-inflammatory activity using the protein denaturation inhibition method with diclofenac sodium as a positive control. The percent of inflammatory inhibition is used to assess sample activity. The results showed that the percent of inflammatory inhibition of Angelica keiskei ethanol extract increased as the test concentration increased. The maximum percent inhibition was obtained at a concentration of 1.5% with the percent of inflammatory inhibition value of 23.14% ± 0.05. Angelica keiskei ethanol extract has anti-inflammatory activity based on in vitro testing using the protein denaturation inhibition method.

References

Almira, D., Subaidah, W. A., Ananto, A. D., Deccati, R. F., & Muliasari, H. (2021). In vitro concentration optimization of ethanol extract from Makasar fruit seeds (Brucea javanica L. Merr) as an anti-inflammatory agent. Jurnal Pijar Mipa, 16(5), 595–599.

Bailey-shaw, Y. a, Williams, L. a D., Green, C. E., Rodney, S., & Smith, A. M. (2017). In-vitro evaluation of the anti-inflammatory potential of selected Jamaican plant extracts using the Bovine Serum albumin protein denaturation assay. International Journal of Pharmaceutical Sciences Review and Research, 47(1), 145–153.

Bhutia, S. (2020). Evaluation of in Vitro Anti-Inflammatory Activity of Citrus Macroptera Montr. Asian Journal of Pharmaceutical and Clinical Research, 13(8), 101–103. https://doi.org/10.22159/ajpcr.2020.v13i8.38063

Bouhlali, E. dine T., Sellam, K., Bammou, M., Alem, C., & Filali-Zehzouti, Y. (2016). In vitro antioxidant and anti-inflammatory properties of selected moroccan medicinal plants. Journal of Applied Pharmaceutical Science, 6(5), 156–162.

Caesar, L. K., & Cech, N. B. (2016). A review of the medicinal uses and pharmacology of ashitaba. Planta Medica, 82(14), 1236–1245.

Chandra, S., Chatterjee, P., Dey, P., & Bhattacharya, S. (2012). Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pacific Journal of Tropical Biomedicine, 2(1 SUPPL.), S178–S180.

Chang, H. R., Lee, H. J., & Ryu, J. H. (2014). Chalcones from angelica keiskei attenuate the inflammatory responses by suppressing nuclear translocation of NF-κB. Journal of Medicinal Food, 17(12), 1306–1313.

Depkes RI. (2000). Parameter Standar Umum Ekstrak Tumbuhan Obat. Direktorat Jendral Pengawasan Obat dan Makanan.

Dewi, N. P. (2020). Uji Kualitatif dan Kuantitatif Metabolit Sekunder Ekstrak Etanol Daun Awar-Awar (Ficus septica Burm.f) dengan Metode Spektrofotometer UV-VIS. Acta Holistica Pharmaciana, 2(1), 16–24.

Fasse, S., & Gottschalk, A. (2023). In Vitro Analysis of the Anti-Inflammatory Activity in Dairy Products Containing Salicornia Europaea. Chemical Engineering Transactions, 102(October), 73–78.

Febrianto, S., Hidayati, A. R., & Muliasari, H. (2023). Aktivitas Antibakteri Fraksi Etil Asetat Dari Ekstrak Metanol Herba Ashitaba (Angelica keiskei) Terhadap Bakteri Staphylococcus epidermidis. Skripsi. Universitas Mataram.

Goldstein, J. L., & Cryer, B. (2015). Gastrointestinal injury associated with NSAID use : a case study and review of risk factors and preventative strategies. Drug, Healthcare, and Patient Safety, 7, 31–41.

Gupta, A., Naraniwal, M., & Kothari, V. (2012). Modern Extraction Methods for Preparation of Bioactive Plant Extracts. 1(1), 8–26.

Hadi, I., Meilian, A. M., & Ulfah, M. (2023). Formulasi Dan Evaluasi Sediaan Salep Ekstrak Etanolik Daun Ubi Jalar Ungu (Ipomoea batatas sp.). Medimuh : Jurnal Kesehatan Muhammadiyah, 4(1), 45–52.

Hamid, A., Hanifa, N. I., & Sunarwidhi, A. L. (2023). Aktivitas Antibakteri Fraksi Ekstrak Metanol Ashitaba (Angelica keiskei) terhadap Staphylococcus aureus. Unram Medical Journal, 12(1), 1283–1290.

Huang, X., He, D., Pan, Z., Luo, G., & Deng, J. (2021). Reactive-oxygen-species- scavenging nanomaterials for resolving inflammation. Materials Today Bio, 11, 100124.

Irawati, L. (2021). Penggunaan Putih Telur Ayam Sebagai Pengganti Bovin Serum Albumin (Bsa) Pada Praktikum Penetapan Protein Metode Lowry. Prosiding 5th Seminar Nasional Penelitian & Pengabdian Kepada Masyarakat 2021, 2(2), 2021.

Katzung, B. G. (2018). Basic & Clinical Pharmacology (14th ed.). McGraw-Hill Education.

Kim, D. W., Curtis-Long, M. J., Yuk, H. J., Wang, Y., Song, Y. H., Jeong, S. H., & Park, K. H. (2014). Quantitative analysis of phenolic metabolites from different parts of Angelica keiskei by HPLC-ESI MS/MS and their xanthine oxidase inhibition. Food Chemistry, 153, 20–27.

Kulla, A. Y., & Chrisandy, G. (2018). Albumin Dari Putih Telur Ayam Dengan Proses Dialisis Dan Pengeringan. Tugas Akhir.

Leksono, W. B., Pramesti, R., Santosa, G. W., & Setyati, W. A. (2018). Jenis Pelarut Metanol Dan N-Heksana Terhadap Aktivitas Antioksidan Ekstrak Rumput Laut Gelidium sp. Dari Pantai Drini Gunungkidul – Yogyakarta. Jurnal Kelautan Tropis, 21(1), 9.

Money, S. (2017). The Risks of Chronic Corticosteroid Exposure. Journal of Pain and Palliative Care Pharmacotherapy, 31(2), 160–161.

Muliasari, H., Hanifa, N. I., Hajrin, W., Andanalusia, M., & Hidayati, A. R. (2023). Determination of Antioxidants by DPPH Scavenging Activity of Ashitaba Herb (Angelica keiskei) Methanol Extract. Jurnal Biologi Tropis, 23(4), 482– 490.

Myers, D. K., Goldberg, A. M., Poth, A., Wolf, M. F., Carraway, J., McKim, J., Coleman, K. P., Hutchinson, R., Brown, R., Krug, H. F., Bahinski, A., & Hartung, T. (2017). From in vivo to in vitro: The medical device testing paradigm shift. Altex, 34(4), 479–500.

Ozdal, T., Capanoglu, E., & Altay, F. (2013). A review on protein – phenolic interactions and associated changes. Food Research International, 51(2), 954– 970.

Pebiansyah, A., Amalia, R., Aulifa, D. L., & Levita, J. (2019). Kadar Kalkon Total Di Dalam Ekstrak Etanol Batang Ashitaba (Angelica keiskei Koidzumi). FITOFARMAKA: Jurnal Ilmiah Farmasi, 9(2), 109–115.

Pranajaya, I. P. A. (2015). Uji Aktivitas Antioksidan Menggunakan Metode Deoksiribosa dan Penetapan Kandungan Fenolik Total pada Fraksi Etil Asetat Ekstrak Etanolik Daun Jambu Mete (Anacardium occidentale L.). Skripsi. Universitas Sanata Dharma.

Ramadhani, M. A., Hati, A. K., Lukitasari, N. F., & Jusman, A. H. (2020). Skrining Fitokimia Dan Penetapan Kadar Flavonoid Total Serta Fenolik Total Ekstrak Daun Insulin (Tithonia diversifolia) Dengan Maserasi Menggunakan Pelarut Etanol 96 %. Indonesian Journal of Pharmacy and Natural Product, 3(1), 8– 18.

Sangeetha, G., & Vidhya, R. (2016). In vitro anti-inflammatory activity of different parts of Pedalium murex (L.). International Journal of Herbal Medicine, 4(3), 31–36.

Setiani, L. A., Sari, B. L., Indriani, L., & Jupersio. (2017). Penentuan Kadar Flavonoid Ekstrak Etanol 70% Kulit Bawang Merah (Allium cepa L.) dengan Metode Maserasi dan MAE (Microwave Assisted Extraction). Fitofarmaka, 7(2), 15–22.

Shukla, S. K., Sharma, A. K., Gupta, V., & Yashavarddhan, M. H. (2019). Pharmacological control of inflammation in wound healing. Journal of Tissue Viability, 28(4), 218–222.

Situmeang, B., Nuraeni, W., Malik Ibrahim, A., & Saronom Silaban, dan. (2016). Analysis of secondary metabolite compounds from leaves extract kesambi (Schleichera oleosa) and antioxidant activity test. Jurnal Pendidikan Kimia, 8(3), 164–168.

Solanum, L., Rahim, F., Elmitra, E., & Abdillah, F. M. (2022). Formulasi Sediaan Bedak Tabur Dari Ekstrak Terpurifikasi Buah Tomat. Prosiding Seminar Kesehatan Perintis E, 5(2), 26–34.

Sun, Y., Liu, Z., & Wang, J. (2011). Ultrasound-assisted extraction of five isoflavones from Iris tectorum Maxim. Separation and Purification Technology, 78(1), 49–54.

Suratmi, S., & Haryanto, S. (2021). Teknik Isolasi Produk Ekstraseluler Dan Intraseluler Dari Bakteri Vibrio harveyi. Buletin Teknik Litkayasa Akuakultur, 19(1), 61.

Wahyudi, L. I. (2023). Optimasi Formula Sediaan Gel Ekstrak Metanol Herba Ashitaba (Angelica Keiskei) Dengan Metode Simplex Lattice Design. Skripsi.

Wijaya, A., & Noviana. (2022). Penetapan Kadar Air Simplisia Daun Kemangi (Ocimum basilicum L.) Berdasarkan Perbedaan Metode Pengeringan. Jurnal Riset Kefarmasian Indonesia, 4(2), 185–195.

Wirasisya, D. G., Hajrin, W., & Muliasari, H. (2018). Aktivitas Antibakteri Ashitaba (Angelica keiskei) terhadap Streptococcus mutans. Unram Medical Journal, 7(2), 16–19.

Zhang, Y., Lee, P., Liang, S., Zhou, Z., Wu, X., Yang, F., & Liang, H. (2015). Structural Basis of Non-Steroidal Anti-Inflammatory Drug Diclofenac Binding to Human Serum Albumin. Chemical Biology and Drug Design, 86(5), 1178–1184.

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.

The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.

Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.

Uji Antiinflamasi Ekstrak Etanol Ashitaba (Angelica keiskei) Secara In Vitro

Authors

DOI:

Published:

Issue:

Keywords:

Articles

Downloads

How to Cite

Downloads

Metrics

Abstract

References

License

Most read articles by the same author(s)

Similar Articles

Menu

Manuscript Template

visitor

Keywords

Mitra Kerja sama

Open Access Journals

Contact Us

Information

Share & Follow