Analysis of Thickness, WVTR, and Microstructure of Whey-Gelatin Protein Based Film with Variation in Chia Seed (Salvia hispanica L.) Concentration
DOI:
10.29303/jbt.v24i2.6919Published:
2024-06-12Downloads
Abstract
Edible film is a thin film that can be eaten or consumed directly and serves as a barrier to water vapour, light, gas, fat, and other solutes. Edible film has biodegradable properties that reduce environmental damage. The purpose of this study was to determine the effect of chia seed addition with different concentrations on the thickness, water vapour transmission rate (WVTR), and microstructure of protein-whey gelatin-based edible film. The study employed a completely randomized design comprising three treatments and three replicates. The treatments comprised the addition of chia seeds at different concentrations, namely C1 (0.05 ml), C2 (0.10 ml) and C3 (0.15 ml). The collected data were analysed using Analysis of Variance (ANOVA), with significant differences being followed by Duncan Multiple Range Test (DMRT). In addition, microstructure measurements were analysed descriptively. The addition of different concentrations of chia seeds resulted in a significant difference (P < 0.01) in the thickness value of whey-gelatin protein-based edible films, but no difference (P > 0.05) in the WVTR of whey-gelatin protein-based edible films. The study produced edible films with thickness values of 0.294–0.304 mm, WVTR of 4.57–5.41 g/mm²/day. The use of chia seed at a concentration of 0.10 ml produces a film with good thickness and WVTR values in comparison to other treatments. The resulting thickness value is 0.302 mm, while the WVTR is 4.57-5.41 g/mm²/day. Furthermore, the film microstructure is denser and more uniform.
Keywords:
Chia Seed; Gelatin; Protein; Whey.References
Acquah, C., Zhang, Y., Dubé, M. A., & Udenigwe, C. C. (2020). Formation and Characterization of Protein-Based Films From Yellow Pea (Pisum sativum) Protein Isolate and Concentrate for Edible Applications. Current Research in Food Science, 14(2): 61-69. DOI: https://doi.org/10.1016/j.crfs.2019.11.008
ASTM E 96 (1995). (1995). Standard Test Methods for Water Vapor Transmission of Materials, E 96/E 96M - 05. ASTM International.
Azevedo, V. M., Silva, E. K., Gonçalves Pereira, C. F., da Costa, J. M. G., & Borges, S. V. (2015). Whey Protein Isolate Biodegradable Films: Influence of The Citric Acid and Montmorillonite Clay Nanoparticles on The Physical Properties. Food Hydrocolloids, 43: 252-258. DOI: https://doi.org/10.1016/j.foodhyd.2014.05.027
Capitani, M. I., Matus-Basto, A., Ruiz-Ruiz, J. C., Santiago-García, J. L., Betancur-Ancona, D. A., Nolasco, S. M., Tomás, M. C., & Segura-Campos, M. R. (2016). Characterization of Biodegradable Films Based on Salvia hispanica L. Protein and Mucilage. Food and Bioprocess Technology, 9(8): 1276–1286. DOI: https://doi.org/10.1007/s11947-016-1717-y
Chen, H., Wang, J., Cheng, Y., Wang, C., Liu, H., Bian, H., Pan, Y., Sun, J., & Han, W. (2019). Application of Protein-Based Films and Coatings For Food Packaging: A Review. Polymers, 11(12): 2039. DOI: https://doi.org/10.3390/polym11122039
Cinelli, P., Schmid, M., Bugnicourt, E., Wildner, J., Bazzichi, A., Anguillesi, I., & Lazzeri, A. (2014). Whey Protein Layer Applied on Biodegradable Packaging Film to Improve Barrier Properties While Maintaining Biodegradability. Polymer Degradation and Stability, 108: 151-157. DOI: https://doi.org/10.1016/j.polymdegradstab.2014.07.007
Cuomo, F., Iacovino, S., Messia, M. C., Sacco, P., & Lopez, F. (2020). Protective Action Of Lemongrass Essential Oil on Mucilage From Chia (Salvia hispanica) Seeds. Food Hydrocolloids, 105. DOI: https://doi.org/10.1016/j.foodhyd.2020.105860
Dick, M., Costa, T. M. H., Gomaa, A., Subirade, M., Rios, A. D. O., & Flôres, S. H. (2015). Edible Film Production from Chia Seed Mucilage: Effect of Glycerol Concentration on Its Physicochemical and Mechanical Properties. Carbohydrate Polymers, 130: 198-205. DOI: https://doi.org/10.1016/j.carbpol.2015.05.040
Dick, M., Pagno, C. H, Costa, T. M. H., Gomaa, A., Subirade, M., Rios, A. D. O., & Flôres, S. H. (2016). Edible Films Based on Chia Flour: Development and Characterization. Journal of Applied Polymer Science, 133(2). DOI: https://doi.org/10.1002/app.42455
Fahrullah, Ervandi, M., Indrianti, M. A., Suparwata, D. O., Yasin, I. A., Gobel, Y. A., Mokoolang, S., & Mokoginta, M. M. (2021). Mechanical Properties of Whey Composite Edible Film wth The Addition of Clove Essential Oil and Different Types of Plasticizer. IOP Conference Series: Earth and Environmental Science, 755(1). DOI: https://doi.org/10.1088/1755-1315/755/1/012004
Fahrullah, Ervandi, M., & Rosyidi, D. (2021). Characterization and Antimicrobial Activity of Whey Edible Film Composite Enriched with Clove Essential Oil. Tropical Animal Science Journal, 44(3): 369-376. DOI: https://doi.org/10.5398/tasj.2021.44.3.369
Fahrullah, F. (2021). Penggunaan Minyak Cengkeh dalam Aplikasi Edible Film Whey Terhadap Karakteristik Kimiawi dan Mikrobiologis Keju Gouda. Agrointek : Jurnal Teknologi Industri Pertanian, 15(2): 592-600. DOI: https://doi.org/10.21107/agrointek.v15i2.10060
Fahrullah, F., Bulkaini, B., Kisworo, D., Yulianto, W., Wulandani, B. R. D., & Haryanto, H. (2023). The Water Content , Solubility , and Optical Properties of Whey-Gelatin Multilayer Films Enriched with Green Tea Powder. Jurnal Biologi Tropis, 23(4): 491–499. DOI: https://doi.org/10.29303/jbt.v23i4.5664
Fahrullah, F., Radiati, L. E., Purwadi, & Rosyidi, D. (2020). The Physical Characteristics of Whey Based Edible Film Added with Konjac. Current Research in Nutrition and Food Science, 8(1): 333-339. DOI: https://doi.org/10.12944/CRNFSJ.8.1.31
Fahrullah, F., & Ervandi, M. (2021). Mikrostruktur Edible Film Whey Dangke dengan Penambahan Karagenan dan Plasticizer Sorbitol 35%. Jurnal Ilmu Dan Teknologi Peternakan Tropis, 6(2): 26–31. DOI: https://doi.org/10.33772/jitro.v8i1.14785
Fahrullah, F., & Ervandi, M. (2022). Karakterisasi mikrostruktur film whey dengan penambahan konjac glucomannan. Agrointek : Jurnal Teknologi Industri Pertanian, 16(3): 403–411. DOI: https://doi.org/10.21107/agrointek.v16i3.12303
Fahrullah, F., Kisworo, D., Bulkaini, B., Yulianto, W., Wulandani, B. R. D., Ulkiyah, K., Kartika, K., & Rahmawati, L. (2024). Optimization of the Thickness , Water Vapour Transmission Rate and Morphology of Protein-Based Films Incorporating Glycerol and Polyethylene Glycol Plasticizers. Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) 34(1): 11–20. DOI: https://doi.org/10.21776/ub.jiip.2024
Fahrullah, F., Kisworo, D., & Noersidiq, A. (2023). Edible Film Based on Whey-Chia Seed: Physical Characterization with Addition of Different Plasticizers. Jurnal Penelitian Pendidikan IPA, 9(10): 8554–8562. DOI: https://doi.org/10.29303/jppipa.v9i10.4978
Fahrullah, F., Kisworo, D., Noersidiq, A., Bulkaini, B., Yulianto, W., Wulandani, B. R. D., & Maslami, V. (2024). Microstructure Characterization of Whey Films Using Different Concentrations of Chia Seeds and 35% Sorbitol as Plasticizer. Jurnal Pijar MIPA, 19(2): 348–352. DOI: https://doi.org/10.29303/jpm.v19i2.6589
Fahrullah, F., Lilik, R., Kartika, K., Kalisom, U., & Wahid, Y. (2023). Effect of whey protein on thickness, water vapour transmission rate, and water content of gelatin film. Jurnal Pijar MIPA, 18(6), 945–949. https://doi.org/10.29303/jpm.v18i6.5680
Fahrullah, F., Noersidiq, A., & Maruddin, F. (2022). Effects of Glycerol Plasticizer on Physical Characteristic of Whey-Konjac Films Enriched with Clove Essential Oil. Journal of Food Quality and Hazards Control, 9(4): 226–233. https://doi.org/10.18502/jfqhc.9.4.11377
Fahrullah, F., Radiati, L. E., Purwadi, P., & Rosyidi, D. (2020). The Effect of Different Plasticizers on the Characteristics of Whey Composite Edible Film. Jurnal Ilmu Dan Teknologi Hasil Ternak, 15(1): 31-37. DOI: https://doi.org/10.21776/ub.jitek.2020.015.01.4
Galus, S., & Kadzińska, J. (2016). Whey Protein Edible Films Modified with Almond And Walnut Oils. Food Hydrocolloids, 52: 78-86. DOI: https://doi.org/10.1016/j.foodhyd.2015.06.013
Hashemi, S. M. B., & Mousavi Khaneghah, A. (2017). Characterization of Novel Basil-Seed Gum Active Edible Films and Coatings Containing Oregano Essential Oil. Progress in Organic Coatings, 110; 35-41. DOI: https://doi.org/10.1016/j.porgcoat.2017.04.041
Hasheminya, S. M., Mokarram, R. R., Ghanbarzadeh, B., Hamishekar, H., Kafil, H. S., & Dehghannya, J. (2019). Development And Characterization Of Biocomposite Films Made From Kefiran, Carboxymethyl Cellulose and Satureja Khuzestanica Essential Oil. Food Chemistry, 289: 443-452. DOI: https://doi.org/10.1016/j.foodchem.2019.03.076
Hasnelly, Nurminabari, I. S., & Nasution, M. E. U. (2015). Pemanfaatan Whey Susu Menjadi Edible Film Sebagai Kemasan Dengan Penambahan CMC, Gelatin dan Plasticizer. Pasundan Food Technology Journal, 2(1): 62-69.
Hosseini, S. F., Rezaei, M., Zandi, M., & Farahmandghavi, F. (2015). Bio-based composite Edible Films Containing Origanum vulgare L. Essential Oil. Industrial Crops and Products, 67: 403-413. DOI: https://doi.org/10.1016/j.indcrop.2015.01.062
Janjarasskul, T., & Suppakul, P. (2018). Active and Intelligent Packaging: The Indication of Quality and Safety. Critical Reviews in Food Science and Nutrition, 58(5): 808-831. DOI: https://doi.org/10.1080/10408398.2016.1225278
Janjarasskul, T., & Tananuwong, K. (2019). Role of Whey Proteins in Food Packaging. Reference Module in Food Science. DOI: https://doi.org/10.1016/b978-0-08-100596-5.22399-8
Jouki, M., Mortazavi, S. A., Yazdi, F. T., & Koocheki, A. (2014). Characterization of Antioxidant-Antibacterial Quince Seed Mucilage Films Containing Thyme Essential Oil. Carbohydrate Polymers, 99: 537-546. DOI: https://doi.org/10.1016/j.carbpol.2013.08.077
Kaewprachu, P., Osako, K., Benjakul, S., Tongdeesoontorn, W., & Rawdkuen, S. (2016). Biodegradable Protein-based Films and Their Properties: A Comparative Study. Packaging Technology and Science, 29(2): 77-90. DOI: https://doi.org/10.1002/pts.2183
Karakoyun, O., & Keskin, S. O. (2023). Effects of Gelatin Concentration ad Co-Plasticizer Type On Physical, Mechanical And Microstructural Properties of Corn Starch-Gelatin Composite Edible Films. Annals of the University Dunarea de Jos of Galati, Fascicle VI: Food Technology, 47(1): 140-153. DOI: https://doi.org/10.35219/foodtechnology.2023.1.09
Khazaei, N., Esmaiili, M., Djomeh, Z. E., Ghasemlou, M., & Jouki, M. (2014). Characterization of New Biodegradable Edible Film Made from Basil Seed (Ocimum basilicum L.) Gum. Carbohydrate Polymers, 102(1): 199-206. DOI: https://doi.org/10.1016/j.carbpol.2013.10.062
Mardiyah, U., Aisyah, J. S. N., & Sandra, L. (2022). Karakterisasi Mikrostruktur Dan Komposisi Unsur Gelatin Ikan Kurisi (Nemipterus bathybius) menggunakan Scanning Electron Microscopy-Energy Dispersive X-Ray (SEM-EDX). JFMR-Journal of Fisheries and Marine Research, 6(2): 19–25. DOI: https://doi.org/10.21776/ub.jfmr.2022.006.02.3
Maruddin, F., Malaka, R., Fahrullah, F., & Taufik, M. (2018). Karakterisitik Edible Film Berbahan Whey Dangke dengan Penambahan Karagenan. Jurnal Veteriner, 19(2): 291-297. DOI: https://doi.org/10.19087/jveteriner.2018.19.2.291
Mihalca, V., Kerezsi, A. D., Weber, A., Gruber‐traub, C., Schmucker, J., Vodnar, D. C., Dulf, F. V., Socaci, S. A., Fărcaș, A., Mureșan, C. I., Suharoschi, R., & Pop, O. L. (2021). Protein‐based Films and Coatings for Food Industry Applications. Polymers, 13(5): 769. DOI: https://doi.org/10.3390/polym13050769
Mishra, D., Khare, P., Singh, D. K., Luqman, S., Ajaya Kumar, P. V., Yadav, A., Das, T., & Saikia, B. K. (2018). Retention of Antibacterial and Antioxidant Properties of Lemongrass Oil Loaded on Cellulose Nanofibre-poly Ethylene Glycol Composite. Industrial Crops and Products, 114: 68-80. DOI: https://doi.org/10.1016/j.indcrop.2018.01.077
Murrieta-Martínez, C. L., Soto-Valdez, H., Pacheco-Aguilar, R., Torres-Arreola, W., Rodríguez-Felix, F., & Márquez Ríos, E. (2018). Edible Protein Films: Sources and Behavior. Packaging Technology and Science, 13(3): 113-122. DOI: https://doi.org/10.1002/pts.2360
Otoni, C. G., Avena-Bustillos, R. J., Azeredo, H. M. C., Lorevice, M. V., Moura, M. R., Mattoso, L. H. C., & McHugh, T. H. (2017). Recent Advances on Edible Films Based on Fruits and Vegetables—A Review. Comprehensive Reviews in Food Science and Food Safety, 16(5): 1151-1169. DOI: https://doi.org/10.1111/1541-4337.12281
Reichert, C. L., Bugnicourt, E., Coltelli, M. B., Cinelli, P., Lazzeri, A., Canesi, I., Braca, F., Martínez, B. M., Alonso, R., Agostinis, L., Verstichel, S., Six, L., De Mets, S., Gómez, E. C., Ißbrücker, C., Geerinck, R., Nettleton, D. F., Campos, I., Sauter, E., … Schmid, M. (2020). Bio-based Packaging: Materials, Modifications, Industrial Applications and Sustainability. Polymers, 12 (7): 1558. DOI: https://doi.org/10.3390/polym12071558
Ribeiro, A. M., Estevinho, B. N., & Rocha, F. (2021). Preparation and Incorporation of Functional Ingredients in Edible Films and Coatings. Food and Bioprocess Technology, 14(2): 209–231. DOI: https://doi.org/10.1007/s11947-020-02528-4
Sabil, S., Maruddin, F., Wahyuni, T., & Taufik, M. (2021). Edible Film Characteristics at Different Casein Concentrations. IOP Conference Series: Earth and Environmental Science, 788(1). DOI: https://doi.org/10.1088/1755-1315/788/1/012115
Safari, A., Kusnandar, F., & Syamsir, E. (2016). Biji Chia : Karakteristik Gum dan Potensi Kesehatannya. Pangan, 25(2): 137-146. DOI: https://doi.org/10.33964/jp.v25i2.329
Sandoval, D. C. G., Sosa, B. L., Martínez-Ávila, G. C. G., Fuentes, H. R., Abarca, V. H. A., & Rojas, R. (2019). Formulation and Characterization of Edible Films Based on Organic Mucilage from Mexican Opuntia ficus-indica. Coatings, 9(8): 506. DOI: https://doi.org/10.3390/coatings9080506
Sapper, M., Wilcaso, P., Santamarina, M. P., Roselló, J., & Chiralt, A. (2018). Antifungal and Functional Properties of Starch-Gellan Films Containing Thyme (Thymus zygis) Essential Oil. Food Control, 92: 505-515. DOI: https://doi.org/10.1016/j.foodcont.2018.05.004
Schmid, M. (2013). Properties of Cast Films Made from Different Ratios of Whey Protein Isolate, Hydrolysed Whey Protein Isolate and Glycerol. Materials, 6(8): 3254-3269. DOI: https://doi.org/10.3390/ma6083254
Schmid, M., & Müller, K. (2018). Whey Protein-based Packaging Films and Coatings. Whey Proteins: From Milk to Medicine. 407-437. DOI: https://doi.org/10.1016/B978-0-12-812124-5.00012-6
Shendurse, A. (2018). Milk Protein Based Edible Films and Coatings–Preparation, Properties and Food Applications. Journal of Nutritional Health & Food Engineering, 8(2): 219-226. DOI: https://doi.org/10.15406/jnhfe.2018.08.00273
Soukoulis, C., Gaiani, C., & Hoffmann, L. (2018). Plant Seed Mucilage as Emerging Biopolymer in Food Industry Applications. Current Opinion in Food Science, 22: 28-42. DOI: https://doi.org/10.1016/j.cofs.2018.01.004
Teixeira, B., Marques, A., Pires, C., Ramos, C., Batista, I., Saraiva, J. A., & Nunes, M. L. (2014). Characterization of Fish Protein Films Incorporated with Essential Oils of Clove, Garlic and Origanum: Physical, Antioxidant and Antibacterial Properties. LWT, 59(1): 533-539. DOI: https://doi.org/10.1016/j.lwt.2014.04.024
Telussa, I., Tanasale, M. F. J. D. P., Ridho, A., Madja, A., & Telussa, I. (2023). Synthesis and Characterization of Biodegradable Plastic from Tropical Marine Microalgae Navicula sp . TAD01. Jurnal Penelitian Pendidikan IPA, 9(9): 6877–6884. DOI: https://doi.org/10.29303/jppipa.v9i9.2107
Timilsena, Y. P., Adhikari, R., Kasapis, S., & Adhikari, B. (2015). Rheological and Microstructural Properties of The Chia Seed Polysaccharide. International Journal of Biological Macromolecules, 81: 991-999. DOI: https://doi.org/10.1016/j.ijbiomac.2015.09.040
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