Modification of Protein-Based Edible Film Characteristics with Different Glycerol Concentrations: A Study on Thickness, Gelation, and Microstructure

Authors

Fahrullah Fahrullah , Basriani Basriani , Cis Anita , Farah Febryanti , Fitri Fitri

DOI:

10.29303/jbt.v24i4.7806

Published:

2024-11-10

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Abstract

A thin layer of material added directly to food products that comes from consumable sources is called an edible film. Proteins can be used to create edible films with barrier qualities against oxygen, moisture, and smell, making them appropriate for use as packaging materials that improve product appearance and have preservation effects. Finding out how different glycerol concentrations affect the thickness, gelation duration, and microstructure of protein-based films is the aim of this investigation. A completely randomized design with three treatments and three replications was used in this investigation. The treatments comprised distinct glycerol concentrations, designated as P1 (35%), P2 (40%), and P3 (45%). The protein-based films exhibited thickness values of 0.108–0.113 mm, gelation times of 16.00–20.67 minutes, and a uniform microstructure. At higher concentrations, such as 45%, glycerol resulted in increased porosity and aggregation within the polymer matrix, which in turn led to a reduction in the homogeneity and mechanical strength of the films.  A glycerol concentration of 35% proved to be the most effective treatment for the production of a whey-gelatin-based edible film, resulting in a smooth surface and an even distribution of glycerol within the protein matrix. This approach effectively reduced the occurrence of cracks or irregularities.

Keywords:

Edible film, gelatin, glycerol, whey.

References

Acquah, C., Zhang, Y., Dubé, M. A., & Udenigwe, C. C. (2020). Formation and characterization of protein-based films from yellow pea (Pisum sativum) protein isolate and concentrate for edible applications. Current Research in Food Science, 2: 61-69. DOI: https://doi.org/10.1016/j.crfs.2019.11.008

Ayunita, N., & Fahrullah, F. (2024). Protein-Based films with different glycerol concentrations: Thickness , gelation time and microstructure. Jurnal Biologi Tropis, 24 (2): 563–569. DOI: https://doi.org/10.29303/jbt.v24i2.6890

Azevedo, V. M., Silva, E. K., Gonçalves Pereira, C. F., da Costa, J. M. G., & Borges, S. V. (2015). Whey protein isolate biodegradable films: Influence of the citric acid and montmorillonite clay nanoparticles on the physical properties. Food Hydrocolloids, 43: 252-258. DOI: https://doi.org/10.1016/j.foodhyd.2014.05.027

Bonilla, J., & Sobral, P. J. A. (2019). Gelatin-chitosan edible film activated with Boldo extract for improving microbiological and antioxidant stability of sliced Prato cheese. International Journal of Food Science and Technology, 54 (5): 1617-1624. DOI: https://doi.org/10.1111/ijfs.14032

Capitani, M. I., Matus-Basto, A., Ruiz-Ruiz, J. C., Santiago-García, J. L., Betancur-Ancona, D. A., Nolasco, S. M., Tomás, M. C., & Segura-Campos, M. R. (2016). Characterization of Biodegradable Films Based on Salvia hispanica L. Protein and Mucilage. Food and Bioprocess Technology, 9 (8): 1276–1286. DOI: https://doi.org/10.1007/s11947-016-1717-y

Chen, H., Wang, J., Cheng, Y., Wang, C., Liu, H., Bian, H., Pan, Y., Sun, J., & Han, W. (2019). Application of protein-based films and coatings for food packaging: A review. Polymers, 11 (12): 2039. DOI: https://doi.org/10.3390/polym11122039

de Jesus, G. L., Baldasso, C., Marcílio, N. R., & Tessaro, I. C. (2020). Demineralized whey–gelatin composite films: Effects of composition on film formation, mechanical, and physical properties. Journal of Applied Polymer Science, 137 (42). DOI: https://doi.org/10.1002/app.49282

Dick, M., Henrique Pagno, C., Haas Costa, T. M., Gomaa, A., Subirade, M., De Oliveira Rios, A., & Hickmann Flôres, S. (2016). Edible films based on chia flour: Development and characterization. Journal of Applied Polymer Science, 133 (2). DOI: https://doi.org/10.1002/app.42455

Fahrullah, Ervandi, M., & Rosyidi, D. (2021). Characterization and antimicrobial activity of whey edible film composite enriched with clove essential oil. Tropical Animal Science Journal, 44 (3): 369-376. DOI: https://doi.org/10.5398/tasj.2021.44.3.369

Fahrullah, F., & Ervandi, M. (2022). Karakterisasi mikrostruktur film whey dengan penambahan konjac glucomannan. Agrointek : Jurnal Teknologi Industri Pertanian, 16 (3): 403-411. https://doi.org/10.21107/agrointek.v16i3.12303

Fahrullah, F., Kisworo, D., Bulkaini, B., Yulianto, W., Wulandani, B. R. D., Ulkiyah, K., Kartika, K., & Rahmawati, L. (2024). Optimization of the thickness , water vapour transmission rate and morphology of protein-based films incorporating glycerol and polyethylene glycol plasticizers. Jurnal Ilmu-Ilmu Peternakan 34 (1): 11-20. DOI: https://doi.org/10.21776/ub.jiip.2024

Fahrullah, F., Kisworo, D., & Noersidiq, A. (2023a). Edible film based on whey-chia seed: physical characterization with addition of different plasticizers. Jurnal Penelitian Pendidikan IPA, 9(10), 8554-8562. DOI: https://doi.org/10.29303/jppipa.v9i10.4978

Fahrullah, F., Kisworo, D., Bulkaini, B., & Haryanto, H. (2023b). The effects of plasticizer types on properties of whey-gelatin films. Jurnal Biologi Tropis 23 (3): 414-421. DOI: http://dx.doi.org/10.29303/jbt.v23i3.5283

Fahrullah, F., Lilik, R., Kartika, K., Kalisom, U., & Wahid, Y. (2023c). Effect of whey protein on thickness, water vapour transmission rate, and water content of gelatin film. Jurnal Pijar MIPA, 18 (6): 945-949. DOI: https://doi.org/10.29303/jpm.v18i6.5680

Fahrullah, F., Noersidiq, A., & Maruddin, F. (2022). Effects of glycerol plasticizer on physical characteristic of whey-konjac films enriched with clove essential oil. Journal of Food Quality and Hazards Control, 9 (4): 226–233. DOI: https://doi.org/10.18502/jfqhc.9.4.11377

Fahrullah, F., Radiati, L. E., Purwadi, & Rosyidi, D. (2020a). The physical characteristics of whey based edible film added with konjac. Current Research in Nutrition and Food Science, 8 (1): 333-339. DOI:https://doi.org/10.12944/CRNFSJ.8.1.31

Fahrullah, F., Radiati, L. E., Purwadi, P., & Rosyidi, D. (2020b). The effect of different plasticizers on the characteristics of whey composite edible film. Jurnal Ilmu dan Teknologi Hasil Ternak, 15 (1): 31-37. DOI: https://doi.org/10.21776/ub.jitek.2020.015.01.4

Farhan, A., & Hani, N. M. (2017). Characterization of edible packaging films based on semi-refined kappa-carrageenan plasticized with glycerol and sorbitol. Food Hydrocolloids, 64: 48-58. DOI: https://doi.org/10.1016/j.foodhyd.2016.10.034

Fitrah, Q., & Fahrullah, F. (2024). Analysis of thickness , WVTR , and microstructure of whey-gelatin protein based film with variation in chia seed ( Salvia hispanica L .) concentration. Jurnal Biologi Tropis, 24 (2): 639–647. DOI: https://doi.org/10.29303/jbt.v24i2.6919

Han, J. W., Ruiz-Garcia, L., Qian, J. P., & Yang, X. T. (2018). Food packaging: A comprehensive review and future trends. Comprehensive Reviews in Food Science and Food Safety, 17 (4): 860-877. DOI: https://doi.org/10.1111/1541-4337.12343

Hosseini, S. F., Rezaei, M., Zandi, M., & Farahmandghavi, F. (2015). Bio-based composite edible films containing Origanum vulgare L. essential oil. Industrial Crops and Products, 67: 403-413. DOI: https://doi.org/10.1016/j.indcrop.2015.01.062

Hosseini, S. F., Rezaei, M., Zandi, M., & Farahmandghavi, F. (2016). Development of bioactive fish gelatin/chitosan nanoparticles composite films with antimicrobial properties. Food Chemistry, 194: 1266-1274. DOI: https://doi.org/10.1016/j.foodchem.2015.09.004

López De Dicastillo, C., Rodríguez, F., Guarda, A., & Galotto, M. J. (2016). Antioxidant films based on cross-linked methyl cellulose and native Chilean berry for food packaging applications. In Carbohydrate Polymers, 136: 1052-1060. DOI: https://doi.org/10.1016/j.carbpol.2015.10.013

Lorevice, M. V., Otoni, C. G., de Moura, M. R., & Mattoso, L. H. C. (2016). Chitosan nanoparticles on the improvement of thermal, barrier, and mechanical properties of high- and low-methyl pectin films. Food Hydrocolloids, 52: 732-740. DOI: https://doi.org/10.1016/j.foodhyd.2015.08.003

Maniglia, B. C., De Paula, R. L., Domingos, J. R., & Tapia-Blácido, D. R. (2015). Turmeric dye extraction residue for use in bioactive film production: Optimization of turmeric film plasticized with glycerol. LWT, 64 (2): 1187-1195. DOI: https://doi.org/10.1016/j.lwt.2015.07.025

Maruddin, F., Ratmawati, R., Fahrullah, F., & Taufik, M. (2018). Karakterisitik edible film berbahan whey dangke dengan penambahan karagenan. Jurnal Veteriner, 19 (2): 291-297. DOI: https://doi.org/10.19087/jveteriner.2018.19.2.291

Mellinas, C., Valdés, A., Ramos, M., Burgos, N., Del Carmen Garrigós, M., & Jiménez, A. (2016). Active edible films: Current state and future trends. Journal of Applied Polymer Science, 133 (2). DOI: https://doi.org/10.1002/app.42631

Otoni, C. G., Avena-Bustillos, R. J., Azeredo, H. M. C., Lorevice, M. V., Moura, M. R., Mattoso, L. H. C., & McHugh, T. H. (2017). Recent advances on edible films based on fruits and vegetables-A Review. Comprehensive Reviews in Food Science and Food Safety, 16 (5): 1151-1169. DOI: https://doi.org/10.1111/1541-4337.12281

Pak, E. S., Ghaghelestani, S. N., & Najafi, M. A. (2020). Preparation and characterization of a new edible film based on Persian gum with glycerol plasticizer. Journal of Food Science and Technology, 57 (9): 3284-3294. https://doi.org/10.1007/s13197-020-04361-1

Pérez, L. M., Piccirilli, G. N., Delorenzi, N. J., & Verdini, R. A. (2016). Effect of different combinations of glycerol and/or trehalose on physical and structural properties of whey protein concentrate-based edible films. Food Hydrocolloids, 56: 352-359. DOI:https://doi.org/10.1016/j.foodhyd.2015.12.037

Ribeiro-Santos, R., de Melo, N. R., Andrade, M., Azevedo, G., Machado, A. V., Carvalho-Costa, D., & Sanches-Silva, A. (2018). Whey protein active films incorporated with a blend of essential oils: Characterization and effectiveness. Packaging Technology and Science, 31 (1): 27-40. DOI: https://doi.org/10.1002/pts.2352

Rusli, A., Metusalach, Salengke, & Tahir, M. M. (2017). Karakterisasi edible film karagenan dengan pemlastis gliserol. Jphpi 2017, 20 (2): 219-229.

Sabil, S., Maruddin, F., Wahyuni, T., & Taufik, M. (2021). Edible film characteristics at different casein concentrations. IOP Conference Series: Earth and Environmental Science, 788(1). https://doi.org/10.1088/1755-1315/788/1/012115

Said, N. S., & Sarbon, N. M. (2022). Physical and Mechanical Characteristics of Gelatin-Based Films as a Potential Food Packaging Material: A Review. Membranes, 12 (5): 442. DOI: https://doi.org/10.3390/membranes12050442

Sanyang, M. L., Sapuan, S. M., Jawaid, M., Ishak, M. R., & Sahari, J. (2016). Effect of plasticizer type and concentration on physical properties of biodegradable films based on sugar palm (arenga pinnata) starch for food packaging. Journal of Food Science and Technology, 53 (1): 326-336. DOI: https://doi.org/10.1007/s13197-015-2009-7

Schmid, M. (2013). Properties of cast films made from different ratios of whey protein isolate, hydrolysed whey protein Isolate and Glycerol. Materials, 6 (8): 3254-3269. DOI: https://doi.org/10.3390/ma6083254

Sitompul, A. J. W. S., & Zubaidah, E. (2017). Pengaruh jenis dan konsentrasi plasticizer terhadap sifat fisik edible film kolang kaling (Arenga pinnata). Jurnal Pangan dan Agroindustri, 5 (1): 13-25.

Soukoulis, C., Gaiani, C., & Hoffmann, L. (2018). Plant seed mucilage as emerging biopolymer in food industry applications. Current Opinion in Food Science, 22: 28-42. DOI: https://doi.org/10.1016/j.cofs.2018.01.004

Suderman, N., Isa, M. I. N., & Sarbon, N. M. (2018). The effect of plasticizers on the functional properties of biodegradable gelatin-based film: A review. Food Bioscience, 24: 111-119. DOI: https://doi.org/10.1016/j.fbio.2018.06.006

Teixeira, B., Marques, A., Pires, C., Ramos, C., Batista, I., Saraiva, J. A., & Nunes, M. L. (2014). Characterization of fish protein films incorporated with essential oils of clove, garlic and origanum: Physical, antioxidant and antibacterial properties. LWT, 59 (1): 533-539. DOI: https://doi.org/10.1016/j.lwt.2014.04.024

Zhang, X., Ma, L., Yu, Y., Zhou, H., Guo, T., Dai, H., & Zhang, Y. (2019). Physico-mechanical and antioxidant properties of gelatin film from rabbit skin incorporated with rosemary acid. Food Packaging and Shelf Life, 19: 121-130. DOI: https://doi.org/10.1016/j.fpsl.2018.12.006

Author Biographies

Fahrullah Fahrullah, Universitas Mataram

Author Origin : Indonesia

Basriani Basriani, Universitas Mataram

Author Origin : Indonesia

Cis Anita, Universitas Mataram

Author Origin : Indonesia

Farah Febryanti, Universitas Mataram

Author Origin : Indonesia

Fitri Fitri, Universitas Mataram

Author Origin : Indonesia

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How to Cite

Fahrullah, F., Basriani, B., Anita, C., Febryanti, F., & Fitri, F. (2024). Modification of Protein-Based Edible Film Characteristics with Different Glycerol Concentrations: A Study on Thickness, Gelation, and Microstructure. Jurnal Biologi Tropis, 24(4), 952–960. https://doi.org/10.29303/jbt.v24i4.7806

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