Regeneration of Cattleya Amazing Thailand in ½ MS Medium with The Addition of Several Concentration of PGR and Coconut Water
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Asnul Fitria , Zozy Aneloi Noli , Djong Hon TjongDOI:
10.29303/jbt.v23i2.6067Published:
2023-12-02Issue:
Vol. 23 No. 2 (2023): Special IssueKeywords:
2,4-D, BAP, Cattleya Amazing Thailand, Coconut water, in vitro.Articles
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Abstract
One of the Cattleya species with aesthetic and commercial value is Cattleya Amazing Thailand. An efficient protocol with a high rate of plantlet regeneration is through in vitro culture. Plantlet regeneration is affected by the addition of a Plant Growth Regulator (PGR). Auxin and cytokinin (2,4-D and BAP) combined with PGR from organic material such as coconut water can increase the growth of leave and roots in plantlet regeneration. The research aims to determine the best PGRs combination in regenerating the Cattleya Amazing Thailand orchid in vitro. The research used the Completely Randomized Design (CRD) experimental method for five treatments. The parameter are survival rate, number of leave, and number of roots. Survival rate was analyzed descriptively and both of number of leave and roots were analyzed statistically. All treatments can form 100% plantlets on survival rate parameter. The treatment of 0.1 mg/L 2,4-D + 1 mg/L BAP gave significantly different results in the number of leaf (9.0) and was not significantly different in the number of root. 0.1 mg/L 2,4-D + 1 mg/L BAP is the best combination for in vitro regeneration of the Cattleya Amazing Thailand orchid.
References
Andaryani, S., Samanhudi, S., & Yunus, A. (2022). Effect of BAP and 2,4-D on callus induction of Jatropha curcas in vitro. Cell Biology and Development, 3(2). https://doi.org/10.13057/cellbioldev/v030202
Apriliyani, R., & Wahidah, B. F. (2021). Perbanyakan anggrek Dendrobium sp. secara in vitro: Faktor-faktor keberhasilannya. Filogeni: Jurnal Mahasiswa Biologi, 1(2). https://doi.org/10.24252/filogeni.v1i2.21992
br Sembiring, S. L. N., Siregar, L. A. M., & Kardhinata, E. H. (2018). Keberhasilan Terbentuknya Tunas Mikro Anggrek (Cattleya Trianae Lindl & Rchb.Fil.) Dalam Beberapa Komposisi Medium. Jurnal Agroekoteknologi, 6(1, Januari).
Colombo, R. C., Hoshino, R. T., Ferrari, E. A. P., Alves, G. A. C., & de Faria, R. T. (2017). Cattleya forbesii x cattleya bowringiana: A new hybrid of cattleya orchid. Crop Breeding and Applied Biotechnology, 17(2). https://doi.org/10.1590/1984-70332017v17n2c28
Devi, H. S., Devi, S. I., & Singh, T. D. (2013). High frequency plant regeneration system of Aerides odorata lour. Through foliar and shoot tip culture. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41(1). https://doi.org/10.15835/nbha4119007
Endang Nurcahyani, Yuliana Permata Sari, Elsi Diana, Sumardi, Hardoko Insan Qudus, & Sri Wahyuningsih. (2022). Analysis of reducing sugar levels of Cattleya sp. orchid plantlet after induction fusaric acid in vitro. World Journal of Advanced Research and Reviews, 14(2). https://doi.org/10.30574/wjarr.2022.14.2.0369
Fatur, H., Rohman, F., Firgiyanto, R., & Selfiana, A. (2023). Pertumbuhan Tanaman Anggrek Cattleya ( Cattleya eximia ) secara In- Vitro pada Media MS dengan Subtitusi NAA dan BAP In-Vitro Growth of Cattleya Orchid ( Cattleya eximia ) On MS Media With NAA And BAP Substitution. 5–7. https://proceedings.polije.ac.id/index.php/agropross/article/view/517/491
Harahap, F., Hariyadi, I., Silitonga, M., Suryani, C., Edi, S., & Ningsih, A. P. (2023). In vitro Growth of Cattleya sp Orchid from Leaf Explants with Growth Regulators. Jurnal Pembelajaran Dan Biologi Nukleus, 9(1). https://doi.org/10.36987/jpbn.v9i1.3945
Hariyadi, I., Harahap, F., & Silitonga, M. (2023). Response Formation of Cattleya Orchid Leaf Callus (Cattleya Sp.) with the Addition of 2.4 Dichlorophenoxy Acetic Acid and 6-Benzyl Amino Purine In Vitro. Science and Technology Applications, 126. https://doi.org/10.4028/p-6rj5x0
Hossen, M., Saha, S., Khatun, F., & Yasmin, S. (2021). Effects of Plant Growth Regulators on In vitro Growth and Development of Orchid Dendrobium sp. from Protocorm Like Bodies (PLBs). Journal of Bangladesh Agricultural University, 19(3). https://doi.org/10.5455/jbau.72286
Isda, M. N., & Fatonah, S. (2014). Induksi Akar Pada Eksplan Tunas Anggrek Grammatophylum scriptum var . citrinum Secara In Vitro Pada Media Ms Dengan Penambahan NAA dan BAP. 7, 53–57. https://doi.org/10.15408/kauniyah.v7i2.2715
Juras, M. C. R., Jorge, J., Pescador, R., De Melo Ferreira, W., Tamaki, V., & Suzuki, R. M. (2019). In vitro culture and acclimatization of Cattleya xanthina (Orchidaceae), an endangered orchid of the Brazilian Atlantic Rainforest. Rodriguesia, 70. https://doi.org/10.1590/2175-7860201970014
Kolachevskaya, O. O., Myakushina, Y. A., Getman, I. A., Lomin, S. N., Deyneko, I. V., Deigraf, S. V., & Romanov, G. A. (2021). Hormonal regulation and crosstalk of auxin/cytokinin signaling pathways in potatoes in vitro and in relation to vegetation or tuberization stages. International Journal of Molecular Sciences, 22(15). https://doi.org/10.3390/ijms22158207
Lawalata, I. J. (2011). Pemberian Beberapa Kombinasi ZPT Terhadap Regenerasi Tanaman Gloxinia (Siningia speciosa) dari Eksplan Batang dan Daun Secara In Vitro. The Journal of Experimental Life Sciences, 1(2), 83–87. https://doi.org/10.21776/ub.jels.2011.001.02.04
Ma, N. L., Khoo, S. C., Lee, J. X., Soon, C. F., & AB Shukor, N. A. (2020). Efficient micropropagation of Dendrobium aurantiacum from shoot explant. Plant Science Today, 7(3), 476–482. https://doi.org/10.14719/PST.2020.7.3.724
Maulia, E., & Basyah, B. (2021). Growth of Patchouli Shoots (Pogostemon Cablin Benth) with Several Concentrations of Growth Regulator Substances in Vitro. Issue 1 Ser. I, 14. http://www.iosrjournals.org/iosr-javs/papers/Vol14-issue1/Series-1/F1401013846.pdf
Mehbub, H., Akter, A., Akter, M. A., Mandal, M. S. H., Hoque, M. A., Tuleja, M., & Mehraj, H. (2022). Tissue Culture in Ornamentals: Cultivation Factors, Propagation Techniques, and Its Application. In Plants (Vol. 11, Issue 23). https://doi.org/10.3390/plants11233208
Mirah, T., Undang, U., Sunarya, Y., & Ermayanti, T. M. (2021). Pengaruh Konsentrasi Sitokinin Dan Jenis Media Terhadap Pertumbuhan Eksplan Buku Stevia (Stevia rebaudiana Bert.) Tetraploid. Media Pertanian, 6(1). https://doi.org/10.37058/mp.v6i1.2893
Murashige, T., & Skoog, F. (1962). A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15(3), 473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Nabila, C. T., Rahmawati, M., & Kesumawati, E. (2022). Pengaruh Konsentrasi 2,4-Dichlorophenoxyacetic Acid dan Benzyl Amino Purin terhadap Induksi Tunas Tanaman Nilam (Pogostemon cablin Benth.) Varietas Tapak Tuan secara In Vitro. Jurnal Ilmiah Mahasiswa Pertanian, 7(4). https://doi.org/10.17969/jimfp.v7i4.22394
Nisa, N. A., Rahayu, T., & Jayanti, G. E. (2021). Peranan BAP dan Air Kelapa pada Medium VW terhadap Organogenesis Dendrobium sp. Metamorfosa: Journal of Biological Sciences, 8(2). https://doi.org/10.24843/metamorfosa.2021.v08.i02.p14
Sulasiah, A., Tumilisar, C., & Lestaria, T. (2015). Pengaruh Pemberian Jenis Dan Konsentrasi Auksin Terhadap Induksi Perakaran Pada Tunas Dendrobium sp Secara In Vitro. BIOMA, 11(2). https://doi.org/10.21009/bioma11(2).5
Tuncer, B. (2021). Development of an efficient in vitro callus proliferation protocol for edible wild rhubarb (Rheum ribes L.). Acta Scientiarum Polonorum, Hortorum Cultus, 20(5). https://doi.org/10.24326/asphc.2021.5.11
Waryastuti, D. E., Setyobudi, L., & Wardiyati, T. (2017). Pengaruh tingkat konsentrasi 2 , 4-D dan Bap pada media MS terhadap induksi kalus embriogenik temulawak (Curcuma Xanthorrhiza Roxb .). Jurnal Produksi Tanaman, 5(1). https://dx.doi.org/10.21176/protan.v5i1.362
Yanti, D., & Isda, M. N. (2021). Induksi tunas dari eksplan nodus jeruk kasturi (citrus microcarpa bunge.) dengan penambahan 6- benzyl amino purine (BAP) secara in vitro. Biospecies, 14(1). https://doi.org/10.22437/biospecies.v14i1.11192
Zhao, Y. (2008). The role of local biosynthesis of auxin and cytokinin in plant development. In Current Opinion in Plant Biology (Vol. 11, Issue 1). https://doi.org/10.1016/j.pbi.2007.10.008
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