Optimizing The Effects of Auxin and Cytokinin Combinations on Shoot and Root Induction of Dendrobium mussauense
DOI:
10.29303/jbt.v24i2.6637Published:
2024-04-30Downloads
Abstract
Dendrobium mussaense, an orchid species of high ornamental value, was subjected to experimental treatments involving the addition of combinations of auxin and cytokinin, namely 2,4-D (2,4-dichlorophenoxyacetic acid) with BAP (6-benzylaminopurine) and 2,4-D with TDZ (thidiazuron). The research aim was to evaluate their effects on the emergence time of shoots and roots. The method used an experiment design with seven treatments. Among the treatments, the most effective was 2,4-D at a concentration of 1 mg/L combined with 0.75 mg/L of BAP. This treatment resulted in the shortest time for both shoot and root emergence. These findings underscore the importance of optimizing hormone combinations for efficient shoot and root induction in D. mussaense, providing valuable insights for orchid propagation and cultivation practices.
Keywords:
Dendrobium mussauense, propagation, root induction, shoot induction.References
Admojo, L., & Indrianto, A. (2016). Pencegahan Browning Fase Inisasi Kalus Pada Kultur Midrib Daun Klon Karet (Hevea brasiliensis Muell Arg). Jurnal Penelitian Karet.34(1),25-34. https://doi.org/10.22302/ppk.jpk.v34i1.220
Apriliyani, R., & Wahidah, B. F. (2021). Perbanyakan anggrek Dendrobium sp. secara in vitro: Faktor-faktor keberhasilannya. Filogeni: Jurnal Mahasiswa Biologi, 1(2), 33–46. https://doi.org/10.24252/filogeni.v1i2.21992
Arli, N. M., & Noli, Z. A. (2023). Induksi Akar Anggrek Dendrobium lasianthera dengan Pemberian Beberapa Konsentrasi Naphthalene Acetic Acid (NAA) Secara In Vitro. Bioscientist: Jurnal Ilmiah Biologi, 11(2),1369–1375. http://dx.doi.org/10.26418/protobiont.v6i3.22494
Ayu, I. A. P. D. I., Fitriani, Y., & Wijana, G. (2022). Inhibition of Dendrobium bicaudatum Reinw. ex Lindl growth using Paclobutrazol for in vitro conservation. Jurnal Hortikultura Indonesia, 13(1), 29–34. https://doi.org/10.29244/jhi.13.1.29-34
Faridah, E., Indrioko, S., & Herawan, T. (2017). Induksi tunas, multiplikasi dan perakaran Gyrinops versteegii (Gilg.) Domke secara in vitro. Jurnal Pemuliaan Tanaman Hutan,11(1),1–13. https://doi.org/10.20886/jpth.2017.11.1.1-13
Febryanti, N. L. P. K., Defiani, M. R., & Astarini, I. A. (2017). Induksi Pertumbuhan Tunas Dari Eksplan Anggrek Dendrobium Heterocarpum Lindl. Dengan Pemberian Hormon Zeatin Dan Naa. Metamorfosa: Journal of Biological Sciences, 4(1). https://doi.org/10.24843/metamorfosa.2017.v04.i01.p07
Fitria, A., Noli, Z. A., & Tjong, D. H. (2023). Regeneration of Cattleya Amazing Thailand in ½ MS Medium with The Addition of Several Concentration of PGR and Coconut Water. Jurnal Biologi Tropis, 23(2), 111–117. DOI: 10.29303/jbt.v23i2.6067
Hajong, S., Kumaria, S., & Tandon, P. (2013). Effect of plant growth regulators on regeneration potential of axenic nodal segments of Dendrobium chrysanthum Journal of Agricultural Science and Technology,15.http://jast.modares.ac.ir/article-23-383 en.html
Hany, I. P., Noli, Z. A., & Idris, M. (2023). Callus Induction of Dendrobium discolor Through The Thin Cell Layer (TCL) Technique Added with 2, 4-Dichlorophenoxyaceticacid. Jurnal Biologi Tropis, 23: 75–80. DOI: 10.29303/jbt.v23i4b.5808
IUCN. (2018). IUCN 2018. The IUCN Red List of Threatened Species. Version 2018-2. https://www.iucnredlist.or. Accested on September 14, 2023.
Kartiman, R., Sukma, D., Aisyah, S. I., & Purwito, A. (2018). Multiplikasi In Vitro Anggrek Hitam (Coelogyne pandurata Lindl.) Pada Perlakuan Kombinasi Naa Dan Bap. Jurnal Bioteknologi & Biosains Indonesia (JBBI), 5(1). https://doi.org/10.29122/jbbi.v5i1.2908
Karyanti, K. (2017). Pengaruh Beberapa Jenis Sitokinin Pada Multiplikasi Tunas Anggrek Vanda douglas Secara In Vitro. Jurnal Bioteknologi & Biosains Indonesia (JBBI), 4(1). https://doi.org/10.29122/jbbi.v4i1.2200
Markal, A., Isda, M. N., & Fatonah, S. (2015). Perbanyakan anggrek Grammatophyllum scriptum (Lindl.) BL. melalui induksi tunas secara in vitro dengan penambahan BAP dan NAA. Riau University. https://jom.unri.ac.id/index.php/JOMFMIPA/article/view/4479
Manuhara Y.S.W. (2014). Kapita Selekta Kultur Jaringan Tumbuhan. Surabaya: Airlangga University Press. https://repository.unair.ac.id/45367/
Media. (2023). Pengaruh Beberapa Knsentrasi Zat Pengatur Tumbuh Dalam Mikropropagasi Anggrek Dendrobium mussauense Secara In Vitro. Thesis Universitas Andalas, Padang.
Mustika, N. D. (2021). Optimasi Kondisi Kultur In Vitro Tanaman Anggrek Dendrobium lineale Rolfe untuk Produksi Bibit Unggul dan Upaya Konservasi. Skripsi , Universitas Gadjah Mada. Yogyakarta.
Nurana, A. R., Wijana, G., & Dwiyani, R. (2017). Pengaruh 2-iP dan NAA terhadap Pertumbuhan Plantlet Anggrek Dendrobium Hibrida pada Tahap Subkultur. Agrotrop, 7(2). https://jurnal.harianregional.com/index.php/agrotrop/article/view/41173.
Nurkapita, N., Linda, R., & Zakiah, Z. (2021). Multiplikasi Eksplan Tunas Anggrek Hitam (Coelogyne pandurata Lindl.) dengan Penambahan NAA (Naphthalene Acetic Acid) dan Ekstrak Biji Jagung (Zea mays) Secara In Vitro. Jurnal Bios Logos, 11(2). https://doi.org/10.35799/jbl.11.2.2021.32674
Puccio, P. (2022). Dendrobium mussauense description and classification https://www.monaconatureencyclopedia.com/dendrobium-mussauense-2/?lang. Diakses Pada 14 September 2023.
Rosmaina, R., & Aryani, D. (2015). Optimasi Naa Dan Bap Terhadap Pertumbuhan Dan Perkembangan Tunas Mikro Tanaman Kantong Semar (Nepenthes Mirabilis) Secara In Vitro. Jurnal Agroteknologi, 5(2). https://doi.org/10.24014/Ja.V5i2.1352
Sakina, S., Anwar, S., & Kusmiyati, F. (2019). Pertumbuhan Planlet Anggrek Dendrobium (Dendrobium sp.) secara In Vitro pada Konsentrasi BAP dan NAA Berbeda. Jurnal Pertanian Tropik, 6(3). https://doi.org/10.32734/jpt.v6i3.3192
Sari, D. I., Suwirmen, & Nasir, N. (2015). Pengaruh Konsentrasi Thidiazuron (TDZ) dan Arang Aktif pada Sub Kultur Tunas Pisang Kepok Hijau (Musa paradisiaca L.). Jurnal Online Ilmu Pengetahuan Alam, 4(3). https://doi.org/10.15294/biosaintifika.v6i1.3785
Viola, Y. R. N., Roviq, M., & Wardiyati, T. (2017). Pengaruh Konsentrasi Ba Terhadap Pembentukan Embrio Somatik Pada Tanaman Kentang (Solanum Tuberosum L.) Secara In Vitro. Journal of Agricultural Science, 2(1). https://jpt.ub.ac.id/index.php/jpt/article/view/123
Yuniati, F., Haryanti, S., & Prihastanti, E. (2018). Pengaruh Hormon dan Ukuran Eksplan terhadap Pertumbuhan Mata Tunas Tanaman Pisang (Musa paradisiaca var. Raja Bulu) Secara In Vitro. Buletin Anatomi Dan Fisiologi, 3(1). https://doi.org/10.14710/baf.3.1.2018.20-28
Yuswanti, H., Dharma, I. P., Utami, U., & Wiraatmaja, I. W. (2015). Mikropropagasi Anggrek Phalaenopsis dengan Menggunakan Eksplan Tangkai Bunga. Agrotrop: Journal on Agriculture Science, 5(2),163–168. https://ojs.unud.ac.id/index.php/agrotrop/article/view/22370
Zulaikha, S., Sarianti, J., Amaria Wulandari, M., Silva, S., Nuron Rizky, Z., Nurokhman, A., & Yachya, A. (2022). Pengaruh 2,4-Dichlorophenoxyacetic Acid (2,4-D) Dan Benzyl Amino Purine (Bap) Terhadap Induksi Tunas Dari Eksplan Folium Dan Petiolus Communis Tanaman Duku (Lansium Domesticum Corr.). STIGMA: Jurnal Matematika Dan Ilmu Pengetahuan Alam Unipa, 15(02). https://doi.org/10.36456/stigma.15.02.6270.52-59
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