Relationship between Proximate Characteristics and pH Value with Total Lactic Acid Bacteria in Etawa Goat Milk
DOI:
10.29303/jbt.v26i2.12044Published:
2026-05-28Downloads
Abstract
Etawa goat milk is a highly nutritious food source with strong potential to be developed as a functional food. Its nutritional components—such as protein, fat, and water content—along with the presence of lactic acid bacteria (LAB), contribute to its health benefits. This study aimed to analyze the proximate characteristics and pH value of Etawa goat milk and to evaluate their relationship with the total LAB count. Milk samples were collected from three locations in Padang City: Gunung Pangilun, Korong Gadang, and Padayo. Proximate analysis was conducted using standard methods from the Indonesian National Standardization Agency, while total LAB was determined using the Total Plate Count method on MRS Agar media. The results showed that moisture content ranged from 86.11–88.93%, protein content from 3.64–4.17%, fat content from 4.00–5.00%, and pH values from 6.70–7.00. The total LAB ranged from 30.66×10⁸ to 48.33×10⁸ CFU/mL, exceeding the minimum threshold required for probiotic sources. Samples with higher protein and fat content tended to have higher LAB counts. In addition, high moisture content and near-neutral pH supported microbial growth. Therefore, the proximate characteristics and pH value of Etawa goat milk are associated with LAB counts, indicating its potential as a natural probiotic source. The findings of this study provide scientific evidence for the utilization of Etawa goat milk as a natural probiotic product and may contribute to the development of dairy-based functional foods and community nutrition programs.
Keywords:
Etawa goat milk Lactic acid bacteria pH Probiotic ProximateReferences
Antarini, A. A. N. (2011). Sinbiotik antara prebiotik dan probiotik. Jurnal Ilmu Gizi, 2(2), 148–155.
Anumudu, C. K., Miri, T., & Onyeaka, H. (2024). Multifunctional applications of lactic acid bacteria: Enhancing safety, quality, and nutritional value in foods and fermented beverages. Foods, 13(23), 3714. https://doi.org/10.3390/foods13233714
Arora, R., Bhojak, N., & Joshi, R. (2013). Comparative aspects of goat and cow milk. International Journal of Engineering Science Invention, 2(1), 7–10. https://www.ijesi.org/papers/Vol(2)1/B210710.pdf
Arum, H. P., & Purwidiani, N. (2014). Pengaruh jumlah ekstrak jahe dan susu skim terhadap sifat organoleptik yoghurt susu kambing Etawa. E-Journal Boga, 3(3), 116–124. https://ejournal.unesa.ac.id/index.php/jurnal-tata-boga/article/view/9041/9020
Badan Standardisasi Nasional. (2006). SNI 01-234.2.2006 Mutu dan Cara Uji Kadar Protein pada Produk Perikanan. BSN.
Badan Standardisasi Nasional. (2011). SNI 01-3141-2011 Susu Segar. BSN.
Badan Standardisasi Nasional. (2015). SNI 2354.2:2015 Mutu dan Cara Uji Kadar Air pada Produk Perikanan. BSN.
Badan Standardisasi Nasional. (2017). SNI 2354.3:2017 Mutu dan Cara Uji Kadar Lemak pada Produk Perikanan. BSN.
Biadała, A., Szablewski, T., Cegielska-Radziejewska, R., Lasik-Kurdyś, M., & Adzahan, N. M. (2023). The evaluation of activity of selected lactic acid bacteria for bioconversion of milk and whey from goat milk to release biomolecules with antibacterial activity. Molecules, 28(9), 3696. https://doi.org/10.3390/molecules28093696
Buckle, K. A., Edwards, R. A., Fleet, G. H., & Wootton, M. (1987). Ilmu Pangan (H. Purnomo & Adiono, Trans.). UI Press.
Canon, F., Maillard, M.-B., Henry, G., Thierry, A., & Gagnaire, V. (2021). Positive interactions between lactic acid bacteria promoted by nitrogen-based nutritional dependencies. Applied and Environmental Microbiology, 87(21), e01055-21. https://doi.org/10.1128/AEM.01055-21
Departemen Kesehatan Republik Indonesia. (2005). Undang-Undang Republik Indonesia Nomor 23 Tahun 2005 tentang Kesehatan. Depkes RI.
Detha, A., Datta, F., Beribe, E., Foeh, N., & Ndaong, N. (2019). Efektivitas bakteri asam laktat yang diisolasi dari susu kuda Sumba terhadap kualitas silase jerami padi. Jurnal Kajian Veteriner, 6(1), 31–37. https://doi.org/10.35508/jkv.v6i1.1053
Fardiaz, S. (1992). Mikrobiologi Pangan I. Gramedia Pustaka Utama.
Food and Agriculture Organization of the United Nations & World Health Organization. (2002). Guidelines for the Evaluation of Probiotics in Food: Report of a Joint FAO/WHO Working Group. FAO/WHO.
García-Cano, I., Rocha-Mendoza, D., Ortega-Anaya, J., Wang, K., Kosmerl, E., & Jiménez-Flores, R. (2019). Lactic acid bacteria isolated from dairy products as potential producers of lipolytic, proteolytic and antibacterial proteins. Applied Microbiology and Biotechnology, 103(13), 5243–5257. https://doi.org/10.1007/s00253-019-09844-6
Hatti-Kaul, R., Chen, L., Dishisha, T., & El Enshasy, H. (2018). Lactic acid bacteria: From starter cultures to producers of chemicals. Journal of Industrial Microbiology & Biotechnology, 45(7), 1–20. https://doi.org/10.1093/femsle/fny213
Hu, M., Wang, D., Tang, X., Zhang, Q., Zhao, J., Mao, B., Zhang, H., & Cui, S. (2024). Improving the utilization efficiency of nitrogen source through co-culture of Lactobacillus strains with different nitrogen source metabolisms. Food Research International, 175, 113745. https://doi.org/10.1016/j.foodres.2023.113745
Jaman, M. F. V., Suada, I. K., & Sampurna, I. P. (2013). Kualitas susu kambing peranakan Etawa selama penyimpanan suhu ruang ditinjau dari rasa, pH dan uji alkohol. Indonesia Medicus Veterinus, 2(5), 469–478. https://ojs.unud.ac.id/index.php/imv/article/view/7845
Kieliszek, M., Pobiega, K., Piwowarek, K., & Kot, A. M. (2021). Characteristics of the proteolytic enzymes produced by lactic acid bacteria. Molecules, 26(7), 1858. https://doi.org/10.3390/molecules26071858
Loforte, Y., Zanzan, M., Cadavez, V., & Gonzales-Barron, U. (2025). Dynamic modelling of Listeria monocytogenes growth in a milk model medium as affected by pH and selected lactic acid bacteria strains. Foods, 14(23), 3999. https://doi.org/10.3390/foods14233999
Martín-Miguélez, J. M., Robledo, J., Martín, I., Castaño, C., Delgado, J., & Córdoba, J. J. (2024). Biocontrol of L. monocytogenes with selected autochthonous lactic acid bacteria in raw milk soft-ripened cheese under different water activity conditions. Foods, 13(1), 172. https://doi.org/10.3390/foods13010172
Melia, S., Yuherman, Ferawati, Jaswandi, Purwanto, H., & Purwati, E. (2018). Kualitas nutrisi dan kandungan mikrobiologi pada susu kerbau, sapi dan kambing dari Sumatera Barat. Jurnal Ilmu Ternak dan Veteriner, 23(3), 150–157. https://doi.org/10.14334/jitv.v23i3.1594
Mirdalisa, C. A., Zakaria, Y., & Nurliana, N. (2016). Efek suhu dan masa simpan terhadap aktivitas antimikroba susu fermentasi dengan Lactobacillus casei. Jurnal Agripet, 16(1), 49–55. https://doi.org/10.17969/agripet.v16i1.3639
Navyanti, F., & Adriyani, R. (2015). Higiene sanitasi, kualitas fisik dan bakteriologi susu sapi segar perusahaan susu X di Surabaya. Jurnal Kesehatan Lingkungan, 8(1), 36–47. https://e-journal.unair.ac.id/JKL/article/view/3098/2252
Ogrodowczyk, A. M., Kowalska, K., & Kulasik, D. (2026). Milk lipids as bioactive modulators of the bacterial proteome: Mechanisms linking dairy management to microbial performance. Animals, 16(3), 477. https://doi.org/10.3390/ani16030477
Paz, N. F., De Oliveira, E. G., De Kairuz, M. S. N., & Ramón, A. N. (2014). Characterization of goat milk and potentially symbiotic non-fat yogurt. Food Science and Technology, 34(3), 629–635. https://doi.org/10.1590/1678-457x.6409
Purnomo, B. (2004). Materi Kuliah Mikrobiologi. Universitas Bengkulu.
Richana, N. (2011). Bioetanol: Bahan Baku, Teknologi, Produksi dan Pengendalian Mutu. Penerbit Nuansa.
Ruvalcaba-Gómez, J. M., Rios-Corripio, M. A., Delgado-Macuil, R. J., Rojas-López, M., & Hernández-Nava, R. G. (2025). Isolation and preliminary screening of lactic acid bacteria for antimicrobial potential from raw milk. Frontiers in Microbiology, 16, 1565016. https://doi.org/10.3389/fmicb.2025.1565016
Saputra, D., & Nurhayati, T. (2013). Produksi dan aplikasi pepton ikan selar untuk media pertumbuhan bakteri. Jurnal Pengolahan Hasil Perikanan Indonesia, 16(3), 215–223. https://doi.org/10.17844/jphpi.v16i3.8059
Silva, B. N., Fernandes, N., Carvalho, L., Faria, A. S., Teixeira, J. A., Rodrigues, C., Gonzales-Barron, U., & Cadavez, V. (2023). Lactic acid bacteria from artisanal raw goat milk cheeses: Technological properties and antimicrobial potential. Italian Journal of Food Safety, 12(4), 11559. https://doi.org/10.4081/ijfs.2023.11559
Sionek, B., Szydłowska, A., Trząskowska, M., & Kołożyn-Krajewska, D. (2024). The impact of physicochemical conditions on lactic acid bacteria survival in food products. Fermentation, 10(6), 298. https://doi.org/10.3390/fermentation10060298
Subagiyo, Margino, S., Hadisaputro, T., & Setyati, W. (2015). Effects of pH, temperature and salinity in growth and organic acid production of lactic acid bacteria isolated from penaeid shrimp intestine. Ilmu Kelautan: Indonesian Journal of Marine Sciences, 20(4), 187–194. https://doi.org/10.14710/ik.ijms.20.4.187-194
Treichel, H., de Oliveira, D., Mazutti, M. A., Di Luccio, M., & Oliveira, J. V. (2010). A review on microbial lipases production. Food and Bioprocess Technology, 3(2). https://doi.org/10.1007/s11947-009-0202-2
Urnemi, Syukur, S., Purwati, E., Ibrahim, S., & Jamsari. (2012). Potensi bakteri asam laktat sebagai kandidat probiotik antimikroba patogen asal fermentasi kakao varietas Criollo. Jurnal Riset Industri, 6(12), 67–76. https://doi.org/10.26578/jrti.v6i12.1519
Wang, J., Wu, T., Lv, R., Shao, W., Hungwe, M., Wang, J., Bai, X., Xie, J., Wang, Y., & Geng, W. (2021). Metabolism characteristics of lactic acid bacteria and the expanding applications in food industry. Frontiers in Bioengineering and Biotechnology, 9, 612285. https://doi.org/10.3389/fbioe.2021.612285
Zakaria, Y., Helmy, M. Y., & Safara, Y. (2011). Analisa kualitas susu kambing peranakan Etawah yang disterilkan pada suhu dan waktu yang berbeda. Agripet, 11(1), 29–34. https://doi.org/10.17969/agripet.v11i1.651
Zapaśnik, A., Sokołowska, B., & Bryła, M. (2022). Role of lactic acid bacteria in food preservation and safety. Foods, 11(9), 1283. https://doi.org/10.3390/foods11091283
Ziarno, M., Zaręba, D., Kowalska, E., & Florowski, T. (2025). A study into the effects of chosen lactic acid bacteria cultures on the quality characteristics of fermented dairy, dairy-oat, and oat beverages. Applied Sciences, 15(7), 3714. https://doi.org/10.3390/app15073714
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