Effectiveness of Cages Inducing Nasopharyngeal Dysplasia in Wistar Rats (Rattus norvegicus)

Gede Rama Hardy Nugraha; Hamsu  Kadriyan; Anak Agung Ayu Niti  Wedayani; Eka Arie  Yuliyani

doi:10.29303/jbt.v23i1.6088

Effectiveness of Cages Inducing Nasopharyngeal Dysplasia in Wistar Rats (Rattus norvegicus)

Authors

Gede Rama Hardy Nugraha , Hamsu Kadriyan , Anak Agung Ayu Niti Wedayani , Eka Arie Yuliyani

DOI:

10.29303/jbt.v23i1.6088

Published:

2023-12-04

Issue:

Vol. 23 No. 1 (2023): Special Issue

Keywords:

Cages, wistar rats, formaldehyde, MDA, nasopharyngeal dysplasia.

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Nugraha, G. R. H., Kadriyan, H. ., Wedayani, A. A. A. N. ., & Yuliyani, E. A. . (2023). Effectiveness of Cages Inducing Nasopharyngeal Dysplasia in Wistar Rats (Rattus norvegicus). Jurnal Biologi Tropis, 23(1), 353–358. https://doi.org/10.29303/jbt.v23i1.6088

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Abstract

The cage is a place used to put experimental animals in experimental studies to study the mechanism of the disease and its response to therapy. Formaldehyde as a carcinogenic compound can increase the occurrence of chronic inflammation which has the potential to increase the growth of cancer cells in experimental animals due to the stress experienced while in the cage. Therefore, the purpose of this study was to compare the effectiveness of large and small cages induced by formaldehyde 30 ppm against nasopharyngeal dysplasia in Wistar rats. This research is an experimental study with a true experimental design with the type of post-test only control group design. This study used 12 male Wistar rats (Rattus norvegicus) which were divided into two groups, namely the group of Wistar rats which were placed in large cages measuring (30 x 24 x 11.5) cm3 of 6 individuals, and in small cages (27 .5 x 21.5 x 9) cm3 of 6 individuals induced by 30 ppm formaldehyde with a span of 6 hours per day for 16 weeks. The rats were terminated and retro-orbital blood samples were taken to measure MDA levels and nasopharyngeal tissue to assess the degree of dysplasia. The results of this study showed that there was a statistically significant difference (p <0.05) between the degrees of nasopharyngeal dysplasia (p=0.003) and MDA levels (p=0.003) in Wistar rats in small cages and large cages. In this study the effectiveness of small cages was greater than large cages in causing dysplasia in the nasopharyngeal tissue of Wistar rats.

References

CDC. (2002). Toxicological Profile for Formaldehyde. ATSDR’s Toxicological Profiles, July, 1–468. DOI: https://doi.org/10.1201/9781420061888_ch87

Dan, S., Pant, M., Kaur, T., & Pant, S. (2020). Toxic Effect of Formaldehyde: a Systematic Review. International Research Journal of Modernization in Engineering Technology and Science, 2(9), 179–189. URL: www.irjmets.com

Eng, J. W., Kokolus, K. M., Reed, C. B., Hylander, B. L., Ma, W. W., & Repasky, E. A. (2014). A Nervous Tumor Microenvironment : The Impact of Adrenergic Stress on A nervous tumor microenvironment : the impact of adrenergic stress on cancer cells , immunosuppression , and immunotherapeutic response. Cancer Immunol Immunother, 63(February 2015), 1115–1128. DOI: https://doi.org/10.1007/s00262-014-1617-9

Eraslan, E., Castelhano-Carlos, M. J., Amorim, L., Soares-Cunha, C., Rodrigues, A. J., & Sousa, N. (2023). Home-cage behavior is impacted by stress exposure in rats. Frontiers in Behavioral Neuroscience, 17(June 2012). DOI: https://doi.org/10.3389/fnbeh.2023.1195011

Garber, J. (2010). Guide for the Care and Use of Laboratory Animals available. American Physiological Society. In National Academies Press.

Ghelli, F., Bellisario, V., Squillacioti, G., Grignani, E., Garzaro, G., Buglisi, M., Bergamaschi, E., & Bono, R. (2021). Oxidative stress induction in woodworkers occupationally exposed to wood dust and formaldehyde. Journal of Occupational Medicine and Toxicology, 16(1), 1–9. DOI: https://doi.org/10.1186/s12995-021-00293-4

Ghelli, F., Cocchi, E., Buglisi, M., Squillacioti, G., Bellisario, V., Bono, R., & Santovito, A. (2021). The role of phase I, phase II, and DNA-repair gene polymorphisms in the damage induced by formaldehyde in pathologists. Scientific Reports, 11(1), 1–8. DOI: https://doi.org/10.1038/s41598-021-89833-w

Hylander, B. L., Repasky, E. A., & Sexton, S. (2022). Using Mice to Model Human Disease: Understanding the Roles of Baseline Housing-Induced and Experimentally Imposed Stresses in Animal Welfare and Experimental Reproducibility. Animals, 12(3), 1–15. DOI: https://doi.org/10.3390/ani12030371

Kang, D. S., Lee, N., Shin, D. Y., Jang, Y. J., & Lee, S. H. (2022). Network ‑ based integrated analysis for toxic effects of high ‑ concentration formaldehyde inhalation exposure through the toxicogenomic approach. Scientific Reports, 1–12. DOI: https://doi.org/10.1038/s41598-022-09673-0

Makin, D. F., Agra, E., Prasad, M., Brown, J. S., Elkabets, M., Menezes, J. F. S., Sargunaraj, F., & Kotler, B. P. (2021). Using Free-Range Laboratory Mice to Explore Foraging, Lifestyle, and Diet Issues in Cancer. Frontiers in Ecology and Evolution, 9(October), 1–12. DOI: https://doi.org/10.3389/fevo.2021.741389

Mutiarahmi, C. N., Hartady, T., & Lesmana, R. (2021). Use of Mice As Experimental Animals in Laboratories That Refer To the Principles of Animal Welfare: a Literature Review. Indonesia Medicus Veterinus, 10(1), 134–145. DOI: https://doi.org/10.19087/imv.2020.10.1.134

OECD. (2018). Series on Testing and Assessment: Guidance document on inhalation toxicity studies No.39, second ed. Paris. Env/Jm/Mono(2009)28/Rev1, 39(95), 1–106. URL: https://www.oecd.org/officialdocuments/publicdisplaydocumentpdf/?cote=env/jm/mono(2009)28/rev1&doclanguage=en

Salthammer, T. (2019). Formaldehyde sources, formaldehyde concentrations and air exchange rates in European housings. Building and Environment, 150(December 2018), 219–232. DOI: https://doi.org/10.1016/j.buildenv.2018.12.042

Sulaksana, M. A., & Kadriyan, H. (2019). Characteristics and Risk Factors of Patients With Nasopharyngeal Carcinoma in West Nusa Tenggara Hospital. International Journal of Nasopharyngeal Carcinoma (Ijnpc), 1(03), 83–85. DOI: https://doi.org/10.32734/ijnpc.v1i03.2058

Susilawati, N. K., Yasa, I. W. P. S., Suardana, W., Maliawan, S., Jawi, I. M., Romdhoni, A. C., Kadriyan, H., Putra, I. W. G. A. E., & Arijana, I. G. K. N. (2022). Purple Sweet Potato Reduces Malondialdehyde and TNF-a, Increases p53, and Protects Histopathological Appearance in Formaldehyde-induced Nasopharyngeal Carcinoma Rats. Indonesian Biomedical Journal, 14(2), 211–217. DOI: https://doi.org/10.18585/inabj.v14i2.1906

Teixeira, M. A., Chaguri, L. C. A. G., Carissimi, A. S., Souza, N. L., Mori, C. M. C., Saldiva, P. H. N., Lemos, M., Macchione, M., Guimarães, E. T., King, M., & Merusse, J. L. B. (2006). Effects of an individually ventilated cage system on the airway integrity of rats (Rattus norvegicus) in a laboratory in Brazil. Laboratory Animals, 40(4), 419–431. DOI: https://doi.org/10.1258/002367706778476398

Wedayani, A. A. A. N., Punagi, A. Q., Pieter, N. A. L., Cangara, H., Sudharmawan, A. A. K., Kadriyan, H., & Andansari, N. (2023). Edukasi Bahaya Paparan Zat Karsinogenik melalui Inhalasi terhadap Risiko terjadinya Keganasan pada Pekerja Pabrik Tembakau di Dengen Daya. Jurnal Pengabdian Magister Pendidikan IPA, 6(1), 284–287.

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Effectiveness of Cages Inducing Nasopharyngeal Dysplasia in Wistar Rats (Rattus norvegicus)

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