Green Synthesis and Stability Testing of Gold Nanoparticles Using a Bioreducer from Red Binahong Leaf Extract (Anredera cordifolia (Ten.) Steenis)
DOI:
10.29303/jpm.v21i5.12087Published:
2026-10-01Downloads
Abstract
Gold nanoparticles are widely used in various fields due to their unique properties. However, the application of gold nanoparticles is greatly influenced by their stability. One factor affecting nanoparticle stability is the use of bioreducers. The synthesis of gold nanoparticles still largely relies on chemicals that can negatively impact the environment, making more environmentally friendly alternatives necessary. One method that can be used is green synthesis, which utilizes natural materials containing active compounds such as polyphenols to reduce Au³⁺ to Au⁰. This study aims to synthesize AuNPs using a green synthesis method with red binahong leaf extract (Anredera cordifolia (Ten.) Steenis) as the bioreductant and 20 ppm HAuCl₄ as the precursor, as well as to evaluate the effect of varying bioreductant volumes (0.25;0.75;1.25 and 2 mL) on the characteristics and stability of the AuNPs during 42 days of storage. Stability observations over 42 days provide information on the long-term stability of AuNPs, which has been reported only to a limited extent in previous studies. Characterization was performed using a UV-Vis spectrophotometer to determine the maximum wavelength and the resulting absorbance values. Particle size was calculated using the Brus cluster equation based on the maximum wavelength values. The results showed the formation of gold nanoparticles, as indicated by a color change in the solution to purple and the appearance of absorption peaks in the 539–576.5 nm range. An increase in bioreductant volume led to higher absorbance, indicating greater nanoparticle formation. The resulting particle size was approximately 21.57–23.39 nm. During storage, absorbance decreased, and the absorption peak shifted, indicating aggregation and changes in particle-size distribution. Among the various bioreductant volumes tested, the optimal volume for stability was 0.75 mL. Based on the research results, the volume of the bioreductant affects the characteristics and stability of the resulting nanoparticles.
Keywords:
Gold Nanoparticles Green Synthesis Red Binahong Leaf Extract Stability of Gold NanoparticlesReferences
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